In vitro starch binding experiments: Study of the proteins related to grain hardness of wheat

Two friabilin components, puroindoline a and GSP-1 were expressed in Escherichia coli. Starch binding properties of the recombinant polypeptides and of friabilin extracted from wheat flour were compared in vitro. The produced proteins as well as native wheat friabilin bound to starch granules prepared from different (soft, hard and durum) wheat cultivars. Starch granules also bound specifically several wheat endosperm proteins other than friabilin

Normalizers of Primitive Permutation Groups

Let $G$ be a transitive normal subgroup of a permutation group $A$ of finite degree $n$. The factor group $A/G$ can be considered as a certain Galois group and one would like to bound its size. One of the results of the paper is that $|A/G| < n$ if $G$ is primitive unless $n = 3^{4}$, $5^4$, $3^8$, $5^8$, or $3^{16}$. This bound is sharp when $n$ is prime. In fact, when $G$ is primitive, $|\mathrm{Out}(G)| < n$ unless $G$ is a member of a given infinite sequence of primitive groups and $n$ is different from the previously listed integers. Many other results of this flavor are established not only for permutation groups but also for linear groups and Galois groups.Comment: 44 pages, grant numbers updated, referee's comments include

The Linear Boltzmann Equation as the Low Density Limit of a Random Schrodinger Equation

We study the evolution of a quantum particle interacting with a random potential in the low density limit (Boltzmann-Grad). The phase space density of the quantum evolution defined through the Husimi function converges weakly to a linear Boltzmann equation with collision kernel given by the full quantum scattering cross section.Comment: 74 pages, 4 figures, (Final version -- typos corrected

Recovering a spinning inspiralling compact binary waveform immersed in LIGO-like noise with spinning templates

We investigate the recovery chances of highly spinning waveforms immersed in LIGO S5-like noise by performing a matched filtering with 10^6 randomly chosen spinning waveforms generated with the LAL package. While the masses of the compact binary are reasonably well recovered (slightly overestimated), the same does not hold true for the spins. We show the best fit matches both in the time-domain and the frequency-domain. These encompass some of the spinning characteristics of the signal, but far less than what would be required to identify the astrophysical parameters of the system. An improvement of the matching method is necessary, though may be difficult due to the noisy signal.Comment: 5 pages, 1 figure + 4 figure panels; Proceedings of the Eight Edoardo Amaldi Conference on Gravitational Waves (Amaldi8), New York, 2009; to be published in J. Phys.: Conf. Series (JPCS

Report of review of St Stephen’s Children’s Centre, Newham: services for children aged up to 3 years

Formation of surface depressions is a significant geological hazard. Prediction of future sinkholes in buried karstic areas needs knowledge about the subsurface. In order to determine the varying topography of the karstifiable bedrock we carried out multielectrode measurements. Due to the hard field conditions, the bedrock depth could not be detected. The resistivity anomalies in some places had a seasonal variation (low-resistivity in springtime, high-resistivity in the end of summer); therefore we interpreted the springtime resistivity lows as indicators of locations with high water content, that is as high porosity, saturated with water. At the same time, when pushing the current- and potential electrodes into the ground, we discovered a regularity in the areal distribution of the soil's rock debris content. Therefore we carried out a systematic electrode-pricking experiment, and categorized the soil's "toughness" corresponding to soft penetration, scratching or blockage within the upper 30 cm. We have found a close relationship between the locations of resistivity- and the soil's toughness extremes. From some epikarstic features we think that high "pricking probe" values indicate smaller depths of the bedrock. The corresponding (springtime) resistivity minima may indirectly indicate more or less collapsed horsts of the carbonate rock

Secret Communication over Broadcast Erasure Channels with State-feedback

We consider a 1-to-$K$ communication scenario, where a source transmits private messages to $K$ receivers through a broadcast erasure channel, and the receivers feed back strictly causally and publicly their channel states after each transmission. We explore the achievable rate region when we require that the message to each receiver remains secret - in the information theoretical sense - from all the other receivers. We characterize the capacity of secure communication in all the cases where the capacity of the 1-to-$K$ communication scenario without the requirement of security is known. As a special case, we characterize the secret-message capacity of a single receiver point-to-point erasure channel with public state-feedback in the presence of a passive eavesdropper. We find that in all cases where we have an exact characterization, we can achieve the capacity by using linear complexity two-phase schemes: in the first phase we create appropriate secret keys, and in the second phase we use them to encrypt each message. We find that the amount of key we need is smaller than the size of the message, and equal to the amount of encrypted message the potential eavesdroppers jointly collect. Moreover, we prove that a dishonest receiver that provides deceptive feedback cannot diminish the rate experienced by the honest receivers. We also develop a converse proof which reflects the two-phase structure of our achievability scheme. As a side result, our technique leads to a new outer bound proof for the non-secure communication problem

AFLP analysis and improved phytoextraction capacity of transgenic gshI-poplar clones (Populus canescens L.) in vitro

Clone stability and in vitro phytoextraction capacity of vegetative clones of R x canescens (2n = 4x = 38) including two transgenic clones (ggs11 and lgl6) were studied as in vitro leaf disc cultures. Presence of the gshI-transgene in the transformed clones was detected in PCR reactions using gshI-specific primers. Clone stability was determined by fAFLP (fluorescent amplified DNA fragment length polymorphism) analysis. In total, 682 AFLP fragments were identified generated by twelve selective primer pairs after EcoRI-MseI digestion. Four fragments generated by EcoAGT-MseCCC were different (99.4% genetic similarity) which proves an unexpectedly low bud mutation frequency in R x canescens. For the study of phytoextraction capacity leaf discs (8 mm) were exposed to a concentration series of ZnSO4 (10(-1) to 10(-5) m) incubated for 21 days on aseptic tissue culture media WPM containing 1 mu m Cu. Zn2+ caused phytotoxicity only at high concentrations (10(-1) to 10(- 2) m). The transgenic poplar cyt-ECS (ggs11) clone, as stimulated by the presence of Zn, showed elevated heavy metal (Cu) uptake as compared to the non-transformed clone. These results suggest that gshI-transgenic poplars may be suitable for phytoremediation of soils contaminated with zinc and copper