52 research outputs found
Energetics and morphology of sockeye salmon: effects of upriver migratory distance and elevation
Resisto and virulotyping of staphylococci from bovine milk samples in Belgium and Norway
Habitat utilization of juvenile Atlantic salmon (Salmo salar L.), brown trout (Salmo trutta L.) and Arctic charr (Salvelinus alpinus (L.)) in two lakes in northern Norway
Small-scale spatial variation in age and size at maturity of stream-dwelling brown trout, Salmo trutta
Susceptibility to lytic bacteriophages and comparison of methicillin resistant staphyloccci from samples colected in European and African Countries, Japan and Canada
Differential Mortality Rates of Fin-Clipped and Carlin-Tagged Roach (Rutilus rutilus L.)
In vitro and in vivo assessment of phage therapy against Staphylococcus aureus causing bovine mastitis
Objectives
The aim of this study was to assess the efficacy of lytic bacteriophages onStaphylococcus aureus causing bovine mastitis, by in vitro and in vivo assays using Galleria mellonella and murine mastitis models.
Methods
Between May and December 2016, tenS. aureus (five methicillin-resistant and five methicillin-sensitive) isolates were isolated from milk samples of cattle with mastitis in Belgium and Norway. The isolates were assessed in vitro for their susceptibility to four lytic bacteriophages (Romulus, Remus, ISP and DSM105264) and subsequently in vivo in G. mellonella larvae and in murine mastitis model.
Results
Romulus, Remus and ISP showed a lytic activity against theS. aureus isolates in vitro. A larvae survival rate below 50% was observed at four days post inoculation in the groups infected with a methicillin-sensitive S. aureus isolate and treated with these three phages in vivo. An incomplete recovery of the mice mastitis was observed at 48 hours post inoculation in the groups infected and treated with the ISP phage in vivo.
Conclusions
The observations are much more pronounced statistically between the infected-PBS treated and infected-phage treated groups inG. mellonella and murine mastitis model demonstrating an effect of the phages against S. aureus associated with bovine mastitis
Effects of seawater-acclimatization and release sites on survival of hatchery-reared brown trout Salmo trutta
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