Neurons containing retrogradely transported Fluoro-Gold exhibit a variety of lysosomal profiles: a combined brightfield, fluorescence, and electron microscopic study.

The advantages of axonally transported Fluoro-Gold as a retrograde fluorescent marker are numerous. The objective of the present study was to determine whether transported Fluoro-Gold is visible in either semi-thin sections for light microscopy or thin sections for electron microscopy. Rats received injections of Fluoro-Gold into either the striatum or thoracic spinal cord. After appropriate survival times, labelled neurons were observed with the fluorescence microscope in brain regions that are known to project to the injected areas. Sections that contained labelled cells were embedded in plastic and examined with a fluorescence microscope. Semi-thin sections of unosmicated tissue displayed high-resolution fluorescent labelling of somata and dendrites. In contrast, osmicated tissue did not fluoresce, but numerous dark granules were observed in the dendritic and perikaryal cytoplasm of labelled neurons in toluidine blue stained sections that were examined with brightfield optics. The unosmicated tissue did not display these granules, and this finding suggested that the granules are composed of membranes. Neurons in other brain regions that are known not to project to the injection sites did not contain these dark granules. Adjacent thin sections examined with the electron microscope displayed numerous electron-dense, lysosome-like organelles in the cytoplasm of labelled neurons. The electron density of these organelles was greater than that of lysosomes in unlabelled neurons. Three types of distinctive organelles were observed in these preparations: (1) relatively dense concentric lamellar bodies of various sizes; (2) heterogeneous or lipofuscin-like lysosomes; and (3) coarse grained lysosomes. Control sections and unlabelled neurons did not display these organelles. Therefore, these organelles appear to correlate with Fluoro-Gold localized within the somata and dendrites of retrogradely labelled neurons. It is not known if they are the Fluoro-Gold itself, or represent a physiological effect on membranes. The results of this study indicate that Fluoro-Gold may be useful for tract tracing at the electron microscopic level

The role of human operators in safety perception of av deployment—insights from a large european survey

Autonomous vehicles are anticipated to play an important role on future mobility offering encouraging solutions to today’s transport problems. However, concerns of the public, which can affect the AVs’ uptake, are yet to be addressed. This study presents relevant findings of an online survey in eight European countries. First, 1639 responses were collected in Spring 2020 on people’s commute, preferred transport mode, willingness to use AVs and demographic details. Data was analyzed for the entire dataset and for vulnerable road users in particular. Results re-confirm the long-lasting discourse on the importance of safety on the acceptance of AVs. Spearman correlations show that age, gender, education level and number of household members have an impact on how people may be using or allowing their children to use the technology, e.g., with or without the presence of a human supervisor in the vehicle. Results on vulnerable road users show the same trend. The elderly would travel in AVs with the presence of a human supervisor. People with disabilities have the same proclivity, however their reactions were more conservative. Next to safety, reliability, affordability, cost, driving pleasure and household size may also impact the uptake of AVs and shall be considered when designing relevant policies

Non-Markovian Dynamics of Charge Carriers in Quantum Dots

We have investigated the dynamics of bound particles in multilevel current-carrying quantum dots. We look specifically in the regime of resonant tunnelling transport, where several channels are available for transport. Through a non-Markovian formalism under the Born approximation, we investigate the real-time evolution of the confined particles including transport-induced decoherence and relaxation. In the case of a coherent superposition between states with different particle number, we find that a Fock-space coherence may be preserved even in the presence of tunneling into and out of the dot. Real-time results are presented for various asymmetries of tunneling rates into different orbitals.Comment: 9 pages, 3 figures, International Workshop on Physics-Based Mathematical Models for Low-Dimensional Semiconductor Nanostructures. BIRS, November 18-23, 200

Persistent Spin Currents in Helimagnets

We demonstrate that weak external magnetic fields generate dissipationless spin currents in the ground state of systems with spiral magnetic order. Our conclusions are based on phenomenological considerations and on microscopic mean-field theory calculations for an illustrative toy model. We speculate on possible applications of this effect in spintronic devices.Comment: 9 pages, 6 figures, updated version as published, Journal referenc

Solitons in polarized double layer quantum Hall systems

A new manifestation of interlayer coherence in strongly polarized double layer quantum Hall systems with total filling factor $\nu=1$ in the presence of a small or zero tunneling is theoretically predicted. It is shown that moving (for small tunneling) and spatially localized (for zero tunneling) stable pseudospin solitons develop which could be interpreted as mobile or static charge-density excitations. The possibility of their experimental observation is also discussed.Comment: Phys. Rev. B (accepted

Spin Effects in a Quantum Ring

Recent experiments are reviewed that explore the spin states of a ring-shaped many-electron quantum dot. Coulomb-blockade spectroscopy is used to access the spin degree of freedom. The Zeeman effect observed for states with successive electron number allows to select possible sequences of spin ground states of the ring. Spin-paired orbital levels can be identified by probing their response to magnetic fields normal to the plane of the ring and electric fields caused by suitable gate voltages. This narrows down the choice of ground-state spin sequences. A gate-controlled singlet--triplet transition is identified and the size of the exchange interaction matrix element is determined.Comment: 13 pages, 3 figures, Proceedings of the QD2004 conference in Banf

Persistent Currents and Dissipation in Narrow Bilayer Quantum Hall Bars

Bilayer quantum Hall states support a flow of nearly dissipationless staggered current which can only decay through collective channels. We study the dominant finite-temperature dissipation mechanism which in narrow bars is driven by thermal nucleation of pseudospin solitons. We find the finite-temperature resistivity, predict the resulting staggered current-voltage characteristics, and calculate the associated zero-temperature critical staggered current and gate voltage.Comment: 4 pgs. REVTeX, 3 eps figure

Incommensurate ground state of double-layer quantum Hall systems

Double-layer quantum Hall systems possess interlayer phase coherence at sufficiently small layer separations, even without interlayer tunneling. When interlayer tunneling is present, application of a sufficiently strong in-plane magnetic field $B_\parallel > B_c$ drives a commensurate-incommensurate (CI) transition to an incommensurate soliton-lattice (SL) state. We calculate the Hartree-Fock ground-state energy of the SL state for all values of $B_\parallel$ within a gradient approximation, and use it to obtain the anisotropic SL stiffness, the Kosterlitz-Thouless melting temperature for the SL, and the SL magnetization. The in-plane differential magnetic susceptibility diverges as $(B_\parallel - B_c)^{-1}$ when the CI transition is approached from the SL state.Comment: 12 pages, 7 figures, to be published in Physical Review

A visual analytics framework for spatio-temporal analysis and modelling

To support analysis and modelling of large amounts of spatio-temporal data having the form of spatially referenced time series (TS) of numeric values, we combine interactive visual techniques with computational methods from machine learning and statistics. Clustering methods and interactive techniques are used to group TS by similarity. Statistical methods for TS modelling are then applied to representative TS derived from the groups of similar TS. The framework includes interactive visual interfaces to a library of modelling methods supporting the selection of a suitable method, adjustment of model parameters, and evaluation of the models obtained. The models can be externally stored, communicated, and used for prediction and in further computational analyses. From the visual analytics perspective, the framework suggests a way to externalize spatio-temporal patterns emerging in the mind of the analyst as a result of interactive visual analysis: the patterns are represented in the form of computer-processable and reusable models. From the statistical analysis perspective, the framework demonstrates how TS analysis and modelling can be supported by interactive visual interfaces, particularly, in a case of numerous TS that are hard to analyse individually. From the application perspective, the framework suggests a way to analyse large numbers of spatial TS with the use of well-established statistical methods for TS analysis