The vertical distribution and biological transport of marine microplastics across the epipelagic and mesopelagic water column.

Plastic waste has been documented in nearly all types of marine environments and has been found in species spanning all levels of marine food webs. Within these marine environments, deep pelagic waters encompass the largest ecosystems on Earth. We lack a comprehensive understanding of the concentrations, cycling, and fate of plastic waste in sub-surface waters, constraining our ability to implement effective, large-scale policy and conservation strategies. We used remotely operated vehicles and engineered purpose-built samplers to collect and examine the distribution of microplastics in the Monterey Bay pelagic ecosystem at water column depths ranging from 5 to 1000 m. Laser Raman spectroscopy was used to identify microplastic particles collected from throughout the deep pelagic water column, with the highest concentrations present at depths between 200 and 600 m. Examination of two abundant particle feeders in this ecosystem, pelagic red crabs (Pleuroncodes planipes) and giant larvaceans (Bathochordaeus stygius), showed that microplastic particles readily flow from the environment into coupled water column and seafloor food webs. Our findings suggest that one of the largest and currently underappreciated reservoirs of marine microplastics may be contained within the water column and animal communities of the deep sea

Structural and Contextual Patterns in Family Health History Knowledge among African American Adults: A Mixed-Methods Social Network Analysis Study*

Background: Family health history is a strong risk factor for many chronic diseases. Ethnic minorities have been found to have a low awareness of their family health history (FHH), which may pose a contributing factor to health disparities. Purpose: The purpose of this mixed-methods social network analysis study was to identify structural and contextual patterns in African American adults’ FHH knowledge based on interpersonal communication exchanges with their family members. Methods: African American adults completed individually administered family network interviews. Participants’ 3-generation family pedigree served as a visual aid to guide their interview. Our primary outcome of interest for this analysis was whether a family member was reported as someone who talks to the participant about their own (i.e., the family member’s) health, which we refer to as a “personal health informant.” To contextualize quantitative findings, participants were asked to describe how they learned about the health history of the relatives they identified during their interview. Results: Participants (n=37) reported an average family network size of 29.4 relatives (SD = 15.5; Range = 10-67). Each participant, on average, named 17% of their familial network as personal health informants. Multivariate regression results showed that participants were more likely to name an alter as a personal health informant if the alter was female (OR = 2.14, p = 0.0519), from the maternal side of the participant’s family (OR = 1.12, p = 0.0006), had one or more chronic health conditions (OR = 2.41, p = 0.0041), was someone who has discussions with the participant about the participant’s health (OR = 16.28, p < 0.0001), was a source of family health information (OR = 3.46, p = 0.0072), and was someone whose health the participant helps to monitor or track (OR = 5.93, p = 0.0002). Complementary qualitative findings indicate that FHH knowledge is facilitated by open, direct communication among relatives. Personal health informants were described as disclosing information for the purposes of informing others for preventive purposes and for gaining social support. Participants also learned about FHH via other methods, including direct observation, during caretaking, and following a relative’s death. Conclusions: Communication and disclosure practices is an important determinant of African Americans’ FHH knowledge. More culturally and contextually meaningful public health efforts are needed to promote family health history sharing, especially regarding paternal family health history, siblings, and extended relatives

Prevalence of microplastics and anthropogenic debris within a deep-sea food web

Microplastic particles (\u3c5 mm) are ubiquitous throughout global marine ecosystems, including the deep sea. Ingestion of microplastics and other anthropogenic microparticles is reported in diverse marine taxa across trophic levels. Trophic transfer, or the movement of microplastics across trophic levels, is reported in laboratory studies but not yet widely measured in marine food webs. The Monterey Bay submarine canyon ecosystem contains a well-studied, known deep-sea food web in which to examine the trophic fate of microplastics. We measured microplastic abundance across 17 genera spanning approximately 5 trophic levels and a diversity of feeding behaviors. Samples were collected using remotely operated vehicles and oblique midwater trawls, and gut contents of all individuals examined (n = 157) were analyzed for microplastic abundance and other anthropogenic particles greater than 100 μm using stereo microscopy. Microparticles were analyzed with Raman spectroscopy to confirm material type. Anthropogenic particles were found in all genera examined, across crustacean, fish, mollusk, and gelatinous organisms, in amounts ranging from 0 to 24 particles per individual. There was no significant relationship between microplastic amount and fish trophic level, suggesting that the trophic transfer of microparticles is not occurring. Body size was positively correlated with microplastic abundance across all taxa. The fish genus Scomber sp. drove this relationship, suggesting higher microparticle abundance in mobile individuals with broad horizontal distributions. Future work should examine physiological pathways for microplastic transport within organisms (e.g. excretion, accumulation on gills, internal translocation of particles) and between organisms within shared habitats to more fully understand the fate of microplastics within aquatic food webs

Author Correction: The vertical distribution and biological transport of marine microplastics across the epipelagic and mesopelagic water column.

An amendment to this paper has been published and can be accessed via a link at the top of the paper

Lineage Abundance Estimation for SARS-CoV-2 in Wastewater Using Transcriptome Quantification Techniques

Effectively monitoring the spread of SARS-CoV-2 mutants is essential to efforts to counter the ongoing pandemic. Predicting lineage abundance from wastewater, however, is technically challenging. We show that by sequencing SARS-CoV-2 RNA in wastewater and applying algorithms initially used for transcriptome quantification, we can estimate lineage abundance in wastewater samples. We find high variability in signal among individual samples, but the overall trends match those observed from sequencing clinical samples. Thus, while clinical sequencing remains a more sensitive technique for population surveillance, wastewater sequencing can be used to monitor trends in mutant prevalence in situations where clinical sequencing is unavailable

Expedited Cellular Uptake of Hydrophobic Antioxidants for Oxidative Stress Mitigation via F127 Micelle Encapsulation

Various hydrophobic antioxidants remain unexplored for the treatment of space radiation and oxidative stress due to their inability to enter the cell without a drug delivery vehicle. Here, we explore the Pluronic F127 drug delivery vehicle, known as a micelle, and examine cellular responses following antioxidant delivery and oxidative stress mitigation. We also investigate methods for producing antioxidant-loaded micelles and their potential suitability for astronauts, including the best ways they might be administered during space flight missions. This research paves the way for new antioxidants to be used to protect astronauts from diseases caused by space conditions such as radiation and microgravity

Cancer of the uterus and treatment of stress incontinence: a pilot study

The purpose of this pilot study was to determine whether women with early-stage endometrial cancer could be screened for stress urinary incontinence (SUI) at their initial gynecologic oncology visit and referred to a urogynecologist for concurrent treatment of their endometrial cancer and SUI