5 research outputs found
Mitochondria-Targeting Ceria Nanoparticles as Antioxidants for Alzheimerās Disease
Mitochondrial oxidative stress is
a key pathologic factor in neurodegenerative
diseases, including Alzheimerās disease. Abnormal generation
of reactive oxygen species (ROS), resulting from mitochondrial dysfunction,
can lead to neuronal cell death. Ceria (CeO<sub>2</sub>) nanoparticles
are known to function as strong and recyclable ROS scavengers by shuttling
between Ce<sup>3+</sup> and Ce<sup>4+</sup> oxidation states. Consequently,
targeting ceria nanoparticles selectively to mitochondria might be
a promising therapeutic approach for neurodegenerative diseases. Here,
we report the design and synthesis of triphenylphosphonium-conjugated
ceria nanoparticles that localize to mitochondria and suppress neuronal
death in a 5XFAD transgenic Alzheimerās disease mouse model.
The triphenylphosphonium-conjugated ceria nanoparticles mitigate reactive
gliosis and morphological mitochondria damage observed in these mice.
Altogether, our data indicate that the triphenylphosphonium-conjugated
ceria nanoparticles are a potential therapeutic candidate for mitochondrial
oxidative stress in Alzheimerās disease
General and Facile Coating of Single Cells via Mild Reduction
Cell surface modification has been
extensively studied to enhance
the efficacy of cell therapy. Still, general accessibility and versatility
are remaining challenges to meet the increasing demand for cell-based
therapy. Herein, we present a facile and universal cell surface modification
method that involves mild reduction of disulfide bonds in cell membrane
protein to thiol groups. The reduced cells are successfully coated
with biomolecules, polymers, and nanoparticles for an assortment of
applications, including rapid cell assembly, in vivo cell monitoring,
and localized cell-based drug delivery. No adverse effect on cellular
morphology, viability, proliferation, and metabolism is observed.
Furthermore, simultaneous coating with polyethylene glycol and dexamethasone-loaded
nanoparticles facilitates enhanced cellular activities in mice, overcoming
immune rejection
Multifunctional Fe<sub>3</sub>O<sub>4</sub>/TaO<sub><i>x</i></sub> Core/Shell Nanoparticles for Simultaneous Magnetic Resonance Imaging and X-ray Computed Tomography
Multimodal imaging is highly desirable for accurate diagnosis
because
it can provide complementary information from each imaging modality.
In this study, a solāgel reaction of tantalumĀ(V) ethoxide in
a microemulsion containing Fe<sub>3</sub>O<sub>4</sub> nanoparticles
(NPs) was used to synthesize multifunctional Fe<sub>3</sub>O<sub>4</sub>/TaO<sub><i>x</i></sub> core/shell NPs, which were biocompatible
and exhibited a prolonged circulation time. When the NPs were intravenously
injected, the tumor-associated vessel was observed using computed
tomography (CT), and magnetic resonance imaging (MRI) revealed the
high and low vascular regions of the tumor
Reversible Cell Layering for Heterogeneous Cell Assembly Mediated by Ionic Cross-Linking of Chitosan and a Functionalized Cell Surface Membrane
Current
heterogeneous cell assembly techniques in coculture systems
rely on irreversible cell layering or a cell separation membrane.
However, the techniques possess major drawbacks of inefficiency in
direct interactions of the assembled cell layers and cell separation
following coculture, which hamper characterization and therapeutic
applications of the cells following coculture. Here, we develop a
reversible cell layering platform for assembly of heterogeneous cells
that allows both active direct cellācell interactions and facile
cell separation. Anionic maleimide-chondroitin-sulfate is grafted
onto the surface membrane of myogenic C2C12 cells and human mesenchymal
stem cells (hMSCs) to modify the surface charge of the cells without
cytotoxicity. A highly porous chitosan thin film is formed <i>in situ</i> interspacing between the heterogeneous cell layers
via ionic cross-linking of cationic chitosan and anionic functionalized
cells, forming compactly assembled double-layered cell constructs.
The chitosan film enables layering of the cells, which allows active
direct interactions between the cell layers, and facile delayering
of the cells through simple treatment with mild shear stress. The
developed platform promotes the myogenic commitment of hMSCs via direct
contact with C2C12 cells, mimicking the interactions that trigger
stem cell differentiation <i>in vivo</i>. Delivery of the
myogenic committed cells to muscle-injured animal models shows evident
muscle regeneration
Self-Assembled Fe<sub>3</sub>O<sub>4</sub> Nanoparticle Clusters as High-Performance Anodes for Lithium Ion Batteries via Geometric Confinement
Although
different kinds of metal oxide nanoparticles continue
to be proposed as anode materials for lithium ion batteries (LIBs),
their cycle life and power density are still not suitable for commercial
applications. Metal oxide nanoparticles have a large storage capacity,
but they suffer from the excessive generation of solidāelectrolyte
interphase (SEI) on the surface, low electrical conductivity, and
mechanical degradation and pulverization resulted from severe volume
expansion during cycling. Herein we present the preparation of mesoporous
iron oxide nanoparticle clusters (MIONCs) by a bottom-up self-assembly
approach and demonstrate that they exhibit excellent cyclic stability
and rate capability derived from their three-dimensional mesoporous
nanostructure. By controlling the geometric configuration, we can
achieve stable interfaces between the electrolyte and active materials,
resulting in SEI formation confined on the outer surface of the MIONCs