Christ For, In, and Through You

...If you would open, if you have your Bible with you in 2 Corinthians 5:14....We are therefore Christ\u27s ambassadors, as though God were making his appeal through us. We implore you on Christ\u27s behalf, be reconciled to God. God made him who had no sin to be sin for us, so that in Him we might become the righteousness of God

Adulterous Nations

In Adulterous Nations, Tatiana Kuzmic enlarges our perspective on the nineteenth-century novel of adultery and how it often served as a metaphor for relationships between the imperial and the colonized. In the context of the long-standing practice of gendering nations as female, the novels discussed—Eliot’s Middlemarch, Fontane’s Effi Briest, and Tolstoy’s Anna Karenina, along with Šenoa’s The Goldsmith’s Gold and Sienkiewicz’s Quo Vadis—can be understood as depicting international crises on the scale of the nuclear family. Kuzmic argues that the hopes, anxieties, and interests of European nations in this period can be discerned in the destabilizing force of adultery. Reading the work of Šenoa and Sienkiewicz, Kuzmic illuminates the relationship between the literature of dominant nations and that of the semicolonized territories that posed a threat to them. Kuzmic’s study enhances our understanding of not only these novels but nineteenth-century European literature more generally

How To Reach This Broken World

...Every single person here this morning is in mission. You are not in remission. You are in mission. There isn\u27t one person here this morning who is not in mission. We are called into mission. We are not in a career. We are in a calling. A holy calling from the Creator God this morning. A holy calling. And every one of us here this morning is gifted. And our gifting and our calling are to be received, unwrapped and given back to the one who gave it to us

Integration of ethics into a forestry curriculum

Following a comprehensive review of their forestry curriculum in 1995, the Oklahoma State University Forestry Faculty elected to modify the way professional ethics are formally addressed. The modifications involve three courses. An introduction of ethics and their role in natural resource management is presented to freshman in an introductory course. This provides a framework for learning and applying the science and practices of the Forestry Profession in the context of an ethical philosophy. Students address ethics a second time between their sophomore and junior years. This happens during the initial summer camp course where students are exposed to philosophical and policy differences between natural resource management agencies. Ethics are formally reintroduced in two senior courses that are usually taken concurrently. One of these courses is a capstone experience where students address real natural resource management problems. The second is a course in forest administration and natural resource policy. In these two courses the instructors cooperate to require the students to consider professional ethics in a philosophical framework for decision making as well as an applied standard for real decisions in the execution of professional work. This is accomplished through discussions of the Ethical Canons of the Society of American Foresters in the forest administration and policy course. These discussions are followed by group presentations to the class of ethical considerations associated with projects from the student’s capstone experience

A Bakery : in search of appropriate form

Thesis (M. Arch.)--Massachusetts Institute of Technology, Dept. of Architecture, 1982.MICROFICHE COPY AVAILABLE IN ARCHIVES AND ROTCH.Includes bibliographical references (leaves 137-139).During the process of designing a building there occurs the continuous task of finding the forms, materials and structure that are fit to the particular building or situation at hand. It is the intent to combine these different elements that make a building and organize them in such as way to reflect a whole, expressive of its meaning. the task is to search for building form or forms that are responsive to the reasons for which they are built and to the people who live in or use them. this thesis is an exploration toward the search for appropriate form. Forms are found and subsequently assembled to fit the situation of a specific building, mapping appropriate design decisions from cues given by the various activities of life the building holds. The design of a medium scale bakery was chose for this exploration. Though the various activities and baking process that occur within a bakery are well documented, the primary intent of this thesis is to design this building as one example to reflect the search for appropriate form. The understanding of the nature of a bakery and how it works combined with the awareness of its users' needs serves as fuel toward this search for a formal morphology fit to this singular building. The actual design exploration or process will be preceded by three chapters. the first chapter is an introduction to appropriate form clarifying or possibly hinting at its meaning. The second chapter documents some built and written references selected to support the meaning as introduces as well as chosen as some building/form examples that have influenced my thinking toward this search for appropriate form. In chapter 3 a particular bakery is used as a prototype because of its unique baking process. This chapter includes the comparison of this particular small scale bakery to larger industrial form examples. the final chapter of this thesis exploration represents the documentation of appropriate form takes place in five phases. the first covers the stronger, more basic reasons and formal definitions toward the design of the bakers. the 5th or final phase documents the overall "unfolding of forms" that make the final building. We are to consider our building, say Ruskin, "as a kind of organized creature." Perhaps this is what I'm trying to do.by Katri Kuzmic.M.Arch

Characteristics of Visitors and Recreational Use of the Upper Kiamichi River Wilderness in Oklahoma

Environmental Scienc

Equilibrium model selection: dTTP induced R1 dimerization

<p>Abstract</p> <p>Background</p> <p>Biochemical equilibria are usually modeled iteratively: given one or a few fitted models, if there is a lack of fit or over fitting, a new model with additional or fewer parameters is then fitted, and the process is repeated. The problem with this approach is that different analysts can propose and select different models and thus extract different binding parameter estimates from the same data. An alternative is to first generate a comprehensive standardized list of plausible models, and to then fit them exhaustively, or semi-exhaustively.</p> <p>Results</p> <p>A framework is presented in which equilibriums are modeled as pairs (<it>g</it>, <it>h</it>) where <it>g </it>= 0 maps total reactant concentrations (system inputs) into free reactant concentrations (system states) which <it>h </it>then maps into expected values of measurements (system outputs). By letting dissociation constants <it>K</it>_{<it>d </it>}be either freely estimated, infinity, zero, or equal to other <it>K</it>_<it>d</it>, and by letting undamaged protein fractions be either freely estimated or 1, many <it>g </it>models are formed. A standard space of <it>g </it>models for ligand-induced protein dimerization equilibria is given. Coupled to an <it>h </it>model, the resulting (<it>g</it>, <it>h</it>) were fitted to dTTP induced R1 dimerization data (R1 is the large subunit of ribonucleotide reductase). Models with the fewest parameters were fitted first. Thereafter, upon fitting a batch, the next batch of models (with one more parameter) was fitted only if the current batch yielded a model that was better (based on the Akaike Information Criterion) than the best model in the previous batch (with one less parameter). Within batches models were fitted in parallel. This semi-exhaustive approach yielded the same best models as an exhaustive model space fit, but in approximately one-fifth the time.</p> <p>Conclusion</p> <p>Comprehensive model space based biochemical equilibrium model selection methods are realizable. Their significance to systems biology as mappings of data into mathematical models warrants their development.</p

Enzyme kinetic and binding studies identify determinants of specificity for the immunomodulatory enzyme ScpA, a C5a inactivating bacterial protease

peer-reviewedArticle was replaced as equations missing from Scheme 1, p. 2359 in original published version - 20210524The Streptococcal C5a peptidase (ScpA) specifically inactivates the human complement factor hC5a, a potent anaphylatoxin recently identified as a therapeutic target for treatment of COVID-19 infections. Biologics used to modulate hC5a are predominantly monoclonal antibodies. Here we present data to support an alternative therapeutic approach based on the specific inactivation of hC5a by ScpA in studies using recombinant hC5a (rhC5a). Initial characterization of ScpA confirmed activity in human serum and against rhC5a desArg (rhC5adR), the predominant hC5a form in blood. A new FRET based enzyme assay showed that ScpA cleaved rhC5a at near physiological concentrations (Km 185 nM). Surface Plasmon Resonance (SPR) and Isothermal Titration Calorimetry (ITC) studies established a high affinity ScpA-rhC5a interaction (KD 34 nM, KITC D 30.8 nM). SPR analyses also showed that substrate binding is dominated (88% of DGbind) by interactions with the bulky N-ter cleavage product (PN, ’core’ residues 1–67) with interactions involving the C-ter R74 contributing most of the remaining DGbind. Furthermore, reduced binding affinity following mutation of a subset of positively charged Arginine residues of PN and in the presence of higher salt concentrations, highlighted the importance of electrostatic interactions. These data provide the first in-depth study of the ScpA-C5a interaction and indicate that ScpA’s ability to efficiently cleave physiological concentrations of C5a is driven by electrostatic interactions between an exosite on the enzyme and the ‘core’ of C5a. The results and methods described herein will facilitate engineering of ScpA to enhance its potential as a therapeutic for excessive immune response to infectious diseas

1988-1992 performance testing of tall fescue turfgrasses at Oklahoma State University, Stillwater

The Oklahoma Cooperative Extension Service periodically issues revisions to its publications. The most current edition is made available. For access to an earlier edition, if available for this title, please contact the Oklahoma State University Library Archives by email at [email protected] or by phone at 405-744-6311

Influence of association state and DNA binding on the O2-reactivity of [4Fe-4S] fumarate and nitrate reduction (FNR) regulator

The fumarate and nitrate reduction (FNR) regulator is the master switch for the transition between anaerobic and aerobic respiration in Escherichia coli. Reaction of dimeric [4Fe-4S] FNR with O2 results in conversion of the cluster into a [2Fe-2S] form, via a [3Fe-4S] intermediate, leading to the loss of DNA binding through dissociation of the dimer into monomers. In the present paper, we report studies of two previously identified variants of FNR, D154A and I151A, in which the form of the cluster is decoupled from the association state. In vivo studies of permanently dimeric D154A FNR show that DNA binding does not affect the rate of cluster incorporation into the apoprotein or the rate of O2-mediated cluster loss. In vitro studies show that O2-mediated cluster conversion for D154A and the permanent monomer I151A FNR is the same as in wild-type FNR, but with altered kinetics. Decoupling leads to an increase in the rate of the [3Fe-4S]1+ into [2Fe-2S]2+ conversion step, consistent with the suggestion that this step drives association state changes in the wild-type protein. We have also shown that DNA-bound FNR reacts more rapidly with O2 than FNR free in solution, implying that transcriptionally active FNR is the preferred target for reaction with O2