The plateau phase of growth of the L-M strain mouse cell in a protein-free medium : III. The effect of added glucose on protein and nucleic acid synthesis and on carborhydrate utilization

The addition of radioactive glucose to suspension cultures of L-M strain mouse cells during the plateau phase has been shown to extend the duration of this period from the normal 48-72 hr to 144-240 hr. Supplemental carbohydrate serves both as an energy source, as detected by the production of radioactive lactic acid and carbon dioxide, and as a precursor for nucleic acid and protein synthesis during this period. On the basis of the specific activities of the nucleic acids and proteins recovered from both the cells and the medium, possibilities of synthesis of multiple proteins and of extracellular RNA are discussed. The addition of radioactive homologous nucleic acidprotein fractions to culture in logarithmic growth is shown to reduce the mean generation time by 50 per cent. The recovery of 12 to 13 per cent of the radioactivity in these same fractions isolated from the cells at the end of logarithmic growth suggests that cells have the potential for re-utilization of cellular material release into the medium from other cells in the population.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/32067/1/0000111.pd

Thaliporphine Preserves Cardiac Function of Endotoxemic Rabbits by Both Directly and Indirectly Attenuating NFκB Signaling Pathway

Cardiac depression in sepsis is associated with the increased morbidity and mortality. Although myofilaments damage, autonomic dysfunction, and apoptosis play roles in sepsis-induced myocardial dysfunction, the underlying mechanism is not clear. All of these possible factors are related to NFκB signaling, which plays the main role in sepsis signaling. Thaliporphine was determined to possess anti-inflammatory and cardioprotective activity by suppressing NFκB signaling in rodents. The purpose of this study is to further prove this protective effect in larger septic animals, and try to find the underlying mechanisms. The systolic and diastolic functions were evaluated in vivo by pressure-volume analysis at different preloads. Both preload-dependent and -independent hemodynamic parameters were performed. Inflammatory factors of whole blood and serum samples were analyzed. Several sepsis-related signaling pathways were also determined at protein level. Changes detected by conductance catheter showed Thaliporphine could recover impaired left ventricular systolic function after 4 hours LPS injection. It could also reverse the LPS induced steeper EDPVR and gentler ESPVR, thus improve Ees, Ea, and PRSW. Thaliporphine may exert this protective effect by decreasing TNFα and caspase3 dependent cell apoptosis, which was consistent with the decreased serum cTnI and LDH concentration. Thaliporphine could protect sepsis-associated myocardial dysfunction in both preload-dependent and -independent ways. It may exert these protective effects by both increase of “good”-PI3K/Akt/mTOR and decrease of “bad”-p38/NFκB pathways, which followed by diminishing TNFα and caspase3 dependent cell apoptosis

Evaluation of a commercial fat depth indicator

Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to [email protected], referencing the URI of the item.Bibliography: leaves 55-59.Not availabl

PHYSIOLOGICAL STUDIES OF DIFFERENTIATION IN MYTILUS EDULIS. I. THE OXYGEN UPTAKE OF ISOLATED BLASTOMERES AND POLAR LOBES

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