Polyethylene imine-based receptor immobilization for label free bioassays

Polyethylene imine (PEI) based immobilization of antibodies is described and the concept is proved on the label free assay of C-Reactive Protein (CRP). This novel immobilization method is composed of a hyperbranched PEI layer which was deposited at a high pH (9.5) on the sensor surface. The free amino groups of PEI were derivatized with neutravidin by Biotin N-hydroxysuccinimide ester and the biotinylated anti-CRP antibody immobilized on this layer. Direct binding assay of recombinant CRP was successfully performed in the low μg/ml concentrations using a label free optical waveguide biosensor

Environmental Studies

With the political turmoil of the late 1960s the concern of human kind was shifted from passiv observer and sufferer of environmental problems to taking an active role in policy making.JRC.E.5-Nuclear chemistr

Characterization and Speciation of Depleted Uranium in Individual Soil Particles Using Microanalytical Methods.

Abstract not availableJRC.E-Institute for Transuranium Elements (Karlsruhe