Odgovor adipokina i srčanog troponina na eksperimentalno izazvanu disfunkciju štitne žlijezde brojlera

This study was conducted to investigate adipokine [Apelin and brain-derived neurotrophic factor (BDNF)] and cardiac troponin (cTnI) response that emerged after adding an essential oil mixture (EOM) (Eucalytus glabutus labii, Thymus vulgaris, Cymbopogon nardus, and Syzygium aromaticum) at different rates to the drinking water of broilers in which thyroid dysfunction (hypo- and hyperthyroidism) had been experimentally induced. In the present study, 150 1-day-old Ross-308 male broiler chicks were used. They were divided into five groups, each with 30 animals. The groups were designed to include five subgroups: control (C), hypothyroid, hypothyroid + 250 ppm EOM, hyperthyroid, and hyperthyroid + 250 ppm EOM, with six animals in each group. At the end of the experiment, Apelin, p-BDNF, cTnI, T3, T4, and thyroid-stimulating hormone (TSH) levels were investigated in blood serum samples obtained by cervical dislocation from four randomly selected animals from each subgroup, making 100 animals in total. It was found that EOM administration resulted in a dose-dependent increase in p-BDNF and apelin levels, and a decrease in T4 levels in the experimentally induced hypo- and hyper-thyroidism groups, but did not affect T3 and cTnI levels. Conversely, an increase in TSH level was observed in the hypothyroidism groups, whereas a decrease was observed in groups with hyperthyroidism. This study is the first to examine adipokine (Apelin and BDNF) and cTnI response to EOM administration in thyroid dysfunction.Cilj rada bio je istražiti odgovor adipokina [apelin i moždani neurotrofni faktor (BDNF)] i srčanog troponina (cTnI) nakon dodavanja različitih omjera mješavine esencijalnih ulja (EOM) (Eucalytus glabutus labii, Thymus vulgaris, Cymbopogon nardus i Syzygium aromaticum) u vodu za piće brojlerima kojima je eksperimentalno izazvana disfunkcija štitnjače (hipotireoidizam i hipertireoidizam). U istraživanju je upotrijebljeno 150 jednodnevnih muških brojlera Ross-308. Podijeljeni su u pet skupina po 30 jedinki. Skupine su osmišljene tako da uključe i pet podskupina sa po šest brojlera: kontrolna skupina (C), hipotireoidna, hipotireoidna s 250 ppm mješavine esencijalnih ulja, hipertireoidna i hipertireoidna s 250 ppm mješavine esencijalnih ulja. Na kraju istraživanja u krvnom su serumu analizirane razine apelina, p-BDNF-a, cTnI-ja, T3, T4 i tireoidnog stimulacijskog hormona (TSH) koji su dobiveni cervikalnom dislokacijom od četiriju nasumično odabranih jedinki iz svake podskupine, što je činilo ukupno 100 životinja. Zapaženo je da je primjena EOM-a uzrokovala porast razina p-BDNF-a i apelina i smanjenje razine T4 u skupinama u kojima su eksperimentalno izazvani hipotireoidizam i hipertireoidizam, ali nije utjecala na razine T3 i cTnI-ija. Nasuprot tomu, u skupinama s hipotireoidizmom zabilježen je porast razine TSH-a, dok je smanjenje razine zabilježeno u skupinama s hipertireoidizmom. Ovo je prvo istraživanje odgovora adipokina (apelina i BDNF-a) i cTnI-ija na primjenu mješavine esencijalnih ulja kod brojlera s disfunkcijom štitne žlijezde

Treatment of rabbit cheyletiellosis with selamectin or ivermectin: a retrospective case study

<p>Abstract</p> <p>Background</p> <p>A retrospective study of rabbits treated against cheyletiellosis was performed to evaluate the efficacy and safety of selamectin or ivermectin in clinical practice.</p> <p>Methods</p> <p>Medical records from 53 rabbits with microscopically confirmed <it>Cheyletiella </it>infestation were collected from two small animal clinics. The rabbits were divided into three groups, based on treatment protocols. Group 1 included 11 rabbits treated with ivermectin injections at 200–476 μg kg^-1subcutaneously 2–3 times, with a mean interval of 11 days. In Group 2, 27 rabbits were treated with a combination of subcutaneous ivermectin injections (range 618–2185 μgkg^-1) and oral ivermectin (range 616–2732 μgkg^-1) administered by the owners, 3–6 times at 10 days interval. The last group (Group 3) included 15 rabbits treated with selamectin spot-on applications of 6.2–20,0 mgkg^-1, 1–3 times with an interval of 2–4 weeks. Follow-up time was 4 months–4.5 years.</p> <p>Results</p> <p>Rabbits in remission were 9/11 (81,8%), 14/27 (51,9%) and 12/15 (80,8%) in groups 1, 2 and 3, respectively.</p> <p>Conclusion</p> <p>All treatment protocols seemed to be sufficiently effective and safe for practice use. Though very high doses were used in Group 2 (ivermectin injections followed by oral administration), the protocol seemed less efficacious compared to ivermectin injections (Group 1) and selamectin spot on (Group 3), respectively, although not statistically significant. Controlled prospective studies including larger groups are needed to further evaluate efficacy of the treatment protocols.</p

Effects of anti-inflammatory and immunosuppressive doses of prednisolone on serum triiodothyronine, thyroxine, and free thyroxine concentrations and thyroid morphology in the dog.

The aim of the present study was to determine the effects of different oral doses of prednisolone (classically used during anti-inflammatory and immunosuppressive therapies with decreasing and/or alternate day protocol scheme) on the serum triiodothyronine (T-3), thyroxine (T-4) and free T-4 (fT(4)) concentrations and thyroid gland morphology

Usage of inactivated microsporum canis vaccine in cats naturally infected with M. canis

The aim: In the present study the aim was to investigate the efficiency of inactivated M. canis vaccine in cats naturally infected with M. canis. Material and methods: 38 long-haired cats at the age of 6 months-6 years, naturally infected with M. canis were included in the present study. At physical examination well-circumscribed areas of alopecia and overflowed circumscribed dark color lesions especially on the head (between ear, pinna and neck), front paws, dorsal and lateral trunk and back, in some cats moist, chapped and itchy lesions and otitis were detected. Microbiological culture from the lesions was positive only for M. canis. The cats were treated with inactivated M. canis vaccine (Biocan-M-Micanfin, Bioveta) 15 days apart for 2 times and 11 cats did not received any therapy. Results: In all of the cats vaccinated the lesions improved gradually within 15 days after vaccination. Complete clinical remission was detected and all cats became culture negative within 28 days. No clinical healing was observed in untreated 11 control cats. By day 70 a new and healthy cat, detected to have no lesions consistent with dermatophytosis and negative culture result, was introduced into the same house within the vaccinated cats. After being introduced the cats were recultured by day 90 and all of the vaccinated cats and the new cat were detected to have negative culture results. No recurrency was observed within 10 months after therapy. Conclusion: Results of the present study indicate that inactivated M. canis vaccine may be a safe and effective therapy for M. canis infection. Copyright © 2006 Cornetis