Characterization of Immunogenic Soluble Crude Proteins from Biomphalaria Pfeifferi Against Schistosoma mansoni

Schistosomiasis is the second most prevalent tropical disease in the world after malaria. Chemotherapy and molluscicides which are the main methods of control do not give lasting protection as the disease continues to spread to other new regions. Previous studies have demonstrated the immunogenic properties of the digestive gland (DG), foot parts (FT) and the rest of body tissue (RT) soluble protein of Biomphalaria pfeifferi against Schistosoma parasite and therefore possible candidates for vaccine development against the parasite. However, information about the chemical composition of the soluble proteins is scanty. The objective of this study was to characterize and determine chemical composition of the DG and FT soluble proteins from Biomphalaria pfeifferi. A total of twelve compounds were identified using GC-MS. N-tert-butyl methylamine and penicillamine were present in both DG and FT soluble proteins. Butylamine S, valine, amino heptanoic acid, 1,1-dimethylamino-1 butane and valienamine were present in the DG soluble protein but were missing in the FT extracts. Tert-butylamine, heptylamine, cycloheptane methylamine, erythro-O-methylthreonine and leucine were present in the FT soluble protein but missing in the DG extracts. FTIR analysis showed N-H stretch at 3100 cm-1, C=O stretch at 1700cm-1, N-H bending at 1600cm-1 and O-H peak at 3500 cm-1 while UV absorption occurred at 240-300 nm thus confirmed the presence of amino acids in the soluble protein extracts. Results from this study justifies medicinal activity of Biomphalaria pfeifferi soluble crude protein extracts. Further studies involving isolation of individual constituents in the crude soluble proteins and subjecting them to bioassay is highly recommended. Keywords: Schistosomiasis; Biomphalaria pfeifferi; Protein extracts; Chemical compounds DOI: 10.7176/JNSR/10-12-03 Publication date:June 30th 202

Characterization of Immunogenic Soluble Crude Proteins from Biomphalaria Pfeifferi Against Schistosoma mansoni

Schistosomiasis is the second most prevalent tropical disease in the world after malaria. Chemotherapy and molluscicides which are the main methods of control do not give lasting protection as the disease continues to spread to other new regions. Previous studies have demonstrated the immunogenic properties of the digestive gland (DG), foot parts (FT) and the rest of body tissue (RT) soluble protein of Biomphalaria pfeifferi against Schistosoma parasite and therefore possible candidates for vaccine development against the parasite. However, information about the chemical composition of the soluble proteins is scanty. The objective of this study was to characterize and determine chemical composition of the DG and FT soluble proteins from Biomphalaria pfeifferi. A total of twelve compounds were identified using GC-MS. N-tert-butyl methylamine and penicillamine were present in both DG and FT soluble proteins. Butylamine S, valine, amino heptanoic acid, 1,1-dimethylamino-1 butane and valienamine were present in the DG soluble protein but were missing in the FT extracts. Tert-butylamine, heptylamine, cycloheptane methylamine, erythro-O-methylthreonine and leucine were present in the FT soluble protein but missing in the DG extracts. FTIR analysis showed N-H stretch at 3100 cm-1, C=O stretch at 1700cm-1, N-H bending at 1600cm-1 and O-H peak at 3500 cm-1 while UV absorption occurred at 240-300 nm thus confirmed the presence of amino acids in the soluble protein extracts. Results from this study justifies medicinal activity of Biomphalaria pfeifferi soluble crude protein extracts. Further studies involving isolation of individual constituents in the crude soluble proteins and subjecting them to bioassay is highly recommended. Keywords: Schistosomiasis; Biomphalaria pfeifferi; Protein extracts; Chemical compounds DOI: 10.7176/JNSR/10-12-03 Publication date:June 30th 202

Profile Of Bacteria And Fungi On Money Coins

Objectives: To determine the quantity and quality of bacterial and fungi on money coins and to identify those that could pose a public health risk.Design: Random sampling of coins from subjects within predetermined categories.Setting: Westlands division of Nairobi Metropolitan province.Subjects: Twenty-shilling coin samples were collected from matatu (a common commuter vehicle in Kenya) taxi conductors, greengrocers, shoe shiners, butchers, food kiosk/restaurant attendants, grocery shops attendants, roast maize vendors and school children. Forty coin samples were analysed for both the total viable content and the types of bacterial and fungal organisms.Results: Average bacterial content on the coins ranged from 2.3xl03 to 25.5x103 and fungi content from 11 to 377 colony forming units. The following potentially pathogenic bacteria were among those isolated: Escherichia coli, Klebsiella, Serratia, Enterobacter, Salmonella,Acinetobacter, Enterococci, Staphylococcus and Bacillus cereus. In addition, this is the first report of potentially pathogenic fungal isolation from money coins. Penicillium spp, Aspergillus niger, Fusarium, Rhizopus, Altenaria spp, Candida spp and Cryptococcus were isolated. Conclusion: Money coins harbour potentially pathogenic bacteria and fungi that may pose a public health risk. Hand hygiene is therefore strongly recommended, especially for those who simultaneously handle food and money

Crop-land Suitability Analysis Using GIS and Remote Sensing in Nyandarua County, Kenya

Land suitability analysis is a method of land evaluation, which measures the degree of appropriateness of land for a certain use. The aim of this research was to identify and delineate the land that can best support potatoes, using GIS-based Multi Criteria Evaluation technique and Remote Sensing. The study was carried out in Nyandarua County in Kenya. Agriculture is the mainstay of local economy in the study area, but the production is very low because some of the crops being introduced are not doing well. There is need therefore to identify and delineate suitable areas for growing various crops to achieve maximum potential yield. Three suitability criteria i.e. soil (PH, texture, depth, drainage), climate (rainfall, temperature) and topography were evaluated based on agronomist experts opinions and FAO guideline for rainfed agriculture. An Analytical Hierarchical Process was used to determine relative importance of criteria and the resulting weights were used to construct the suitability maps/layers using GIS software. Finally, land suitability map was generated by overlaying these maps with current land cover map generated from Landsat images through supervised classification. The results of this research revealed that in the study area, 37.6% of the agricultural land is highly suitable for potatoes cultivation, 51.5% is moderately suitable and 10.9% is marginally suitable. The results can be used by the County government to advice the local farmers on the suitable areas for potatoes cultivation. Keywords: Land suitability analysis, GIS, Remote sensing, Multi-Criteria Evaluation, AHP, Potatoes cultivation, Nyandarua

Optimization of FTA technology for large scale plant DNA isolation for use in marker assisted selection

Conventional methods for DNA acquisition and storage require expensive reagents and equipments. Experimental fields located in remote areas and large sample size presents greater challenge to developing country institutions constrained financially. FTATM technology uses a single format utilizing basic tools found in laboratory. In this study, FTATM cards were used to collect over 3000 samples from a field located 130 km and used to screen progeny plants using PCR-marker-assisted selection. PCR product yields and quality are sufficient for reliable scoring, distinguishing heterozygous fromhomozygous plants using ABI 3730 sequencer. Results indicate that this method is faster, easier and in-expensive

A comparison of two sampling methods for surveying mammalian herbivore impacts on beetle communities in the canopy of Acacia drepanolobium in Kenya

Even though several methods are used to sample and monitor canopy arthropods, there are no studies to indicate which of these methods is more effective.We compared the efficacy of the beating and canopy fogging methods in collecting beetles that inhabit Acacia drepanolobium (Harms) tree canopies at Mpala Research Centre in Laikipia district, Kenya. These trees grow naturally on the black cotton soils of the Laikipia ecosystem, accounting for more than 98%of the overstorey at the study site, and are important for local cattle and wildlife production. The ultimate objective of this study was to determine the effect of differential grazing and browsing pressure from large mammalian herbivores on the beetle communities of A. drepanolobium. Seven hundred and twenty trees 1.0–2.5 m tall were sampled using each method, making a total of 1440 trees. Sampling using the two methods was done concurrently and repeated quarterly over a period of 14 months. In total, 4320 individuals were collected, 1456 by beating and 2864 by fogging. The methods jointly yielded beetle specimens from 13 families and 55 morphospecies. Fogging collected significantly more beetle morphospecies than beating, and there was a significant interaction effect between method and sampling date. We found that numerically Anthicidae and Curculionidae responded positively to the presence of cattle.We also found that Anthicidae sp. A and Myllocerus sp. A numbers significantly increased in plots where livestock were grazed

Marker assisted introgression of opaque2 gene into herbicide resistant elite maize inbred lines

Marker assisted selection in combination with conventional breeding can greatly accelerate the introgression of modified opaque2 genotype into herbicide resistant maize. By combining these two approaches, time and costs are greatly minimized. The application of opaque2 allele specific SSR markers was done on materials already undergoing selection in a breeding program for converting herbicide resistant maize lines into quality protein maize (QPM) which is the equivalent of modified opaque2 phenotype. The breeder had selected QPM lines using the light table in the previous cycle and we used leaf samples to extract DNA for analysis of the presence of the opaque2 gene using SSR markers. Two co-dominant SSR markers phi057 and umc1066 and a dominant marker phi112 were used. Umc1216, a modifier marker was also tested in combination with the opaque2 markers with theobjective of using the marker to select for modifiers for the opaque2 phenotype. The modified FTA paper technology protocol was applied in field sampling. The results showed 97% of the lines wereopaque2 while 3% were non-opaque2. Both methods of conventional breeding using light table and marker assisted selection (MAS) were comparable. However, the application of SSR markers and theFTA technology offers the breeder a fast, time saving, reliable and less labour intensive method of screening QPM maize during the early growing stages instead of having to wait to screen the kernels onthe light table after harvesting. Moreover, the routine biochemical analysis for high lysine and tryptophan levels need not be carried out at each backcross since the presence of the opaque2 gene is confirmed with markers

Diversity and Taxonomic Identity of Rumen Bacterial Community in Cattle Fed Different Diets

In this study, the identity of rumen bacterial community and their taxonomic classification in Zebu heifers (n=24) fed 4 different diets (range grasses, Bracharia MulatoII, Azolla and Cassava leaf meal) was surveyed using metagenomics sequencing of the 16SrRNA gene. Rumen liquor samples were collected from the heifers from which a total of 192 DNA samples were amplified and the resulting 16S rRNA sequences compared to the existing sequences at the National Centre for Biotechnology Information (NCBI) BLAST database through the MetagenAssist . Bioinformatics analyses indicated that 17 operational taxonomic units (OTUs) were present at the phylum level. Of these, 43.3% were affiliated to the phylum Firmicutes, 27.2% Bacteroidetes, 22.8% Proteobacteria and 1.7% Euryarchaeota. The remaining were Cyanobacteria (1.4%), Chloroflexi (1%) while Actinobacteria, Verrucomicrobia, Spirochaetes, Tenericutes, Planctomycetes, Elusimicrobia, Lentisphaerae, Armatimonadetes, Fibrobacteres, Synergistetes and Arthropoda were all below 1%. Both Time and Diet had significant effect on the abundance of microbes but did not affect their diversity. Different diets therefore can affect the abundance of rumen microbiome and eventually the performance of animals

Determinants of the Effectiveness of Antimicrobial Prophylaxis among Neurotrauma Patients at a Referral Hospital in Kenya: Findings and Implications

Background: Surgical site infections can occur adding to morbidity, mortality and costs, and can be particularly problematic in low and middle income countries. This includes infections in neurosurgical patients following surgery despite antimicrobial prophylaxis. The study aimed at measuring the incidence of surgical site infections (SSIs) and identifying factors that influence the effectiveness of antimicrobial prophylaxis in a leading hospital in Kenya. Methods: Prospective cohort study from April to July 2015 in the Neurosurgical ward of a leading referral hospital in Kenya. Adult head injury patients were recruited by universal sampling. Data was collected on prophylactic antibiotics and the occurrence of SSIs. Risk factors for infection were identified by logistic regression. Results: Eighty four patients were recruited, with 69 patients eventually analysed. Incidence of SSIs was 37.7% (n=26). The most common antibiotic used for prophylaxis was ceftriaxone. Patients on prophylaxis were less likely to be infected than those who did not receive prophylaxis; however, this was not statistically significant (RR 0.87, 95% CI 0.40-1.893). The presence of epidural haematoma was a risk factor for the development of SSIs (Crude RR 2.456, 95% CI 1.474-4.090). Overall, antimicrobial prophylaxis was effective only in patients who underwent evacuation of hematoma by craniotomy (risk reduction, 62.5% (CI, 29.0% -96.0%). Conclusion: Evacuation of haematomas through craniotomy increased the effectiveness of prophylaxis, and should be considered in the future. The rationale will be explored further to see if antibiotic prophylaxis can reduce SSIs in other patients with neuro trauma