Produktivitas Ayam Buras Hasil Seleksi Berdasarkan Pengetahuan Lokal Peternak

Penggalian potensi ayam buras (kampung) menjadi semakin penting pada kondisikrisis ekonomi seperti sekarang. Hal tersebut menyebabkan kita perlu menengokpotensi yang secara sosial diterima, secara ekonomi terjangkau dan secarateknologis mulai dikembangkan dan mudah diterapkan. Namun di pihak laintingkat produktivitasnya masih rendah karena sistem pemeliharaan danseleksinya yang kurang berkembang. Sistem pengetahuan lokal cara seleksi padamasyarakat pedesaan sebenarnya ada hanya kurang mendapat perhatian danminat para akademisi seperti pengetahuan Catur Rangga yang belum banyakdielaborasi. Tujuan dari penelitian ini adalah: a) Untuk menganalisis produktivitasayam buras hasil seleksi; b) Untuk menganalisis pengetahuan lokal peternakmengenai ayam buras; c) Untuk menganalisis hubungan antara produktivitasayam buras hasil seleksi dengan pengetahuan lokal peternak. Metode yangdigunakan dalam penelitian ini studi kasus dengan teknik PRA (Praticipation RuralAppraisal) partisipasi anggota kelompok melalui pola FGD (Focus GroupDiscussion). Data yang diambil untuk pengembangan sistem pengetahuan lokalberdasarkan variabel-variabel: (1) Sistem pengetahuan lokal, dengan parameter:a) Tulang; b) Bulu; c) Jengger; d) Kaki; e) Mata; f) Kloaka; g) Tulang dubur; h)Jari kaki; i) Kepala; Punggung. (2) Produktivitas, dengan parameter data produksitelur per bulan. Metode analisis yang digunakan adalah Uji Rank Spearman(Siegel, 1997) dan interprestasi dengan Guilford (Rakhmat, 1986). Kesimpulandari hasil penelitian ini adalah: a) Produktivitas ayam buras hasil seleksiditunjukkan oleh nilai rata-rata produksi telur 20,45/butir/bulan; b) Pengetahuanlokal peternak mengenai ayam buras sebagian besar searah dengan ilmupengetahuan modern, yang pada mulanya dikonsepsikan dengan Catur Ranggauntuk ayam adu kemudian juga digunakan untuk ayam produksi; c) Hubunganantara produktivitas ayam buras dengan pengetahuan lokal: untuk produksi ratarataproduksi telur/bulan menunjukkan hubungan yang sangat tinggi. Saran yangdiajukan bahwa parameter dari pengetahuan lokal dapat dijadikan salah satumetode untuk mengetahui produktivitas ayam buras di tingkat peternak; perludilakukan penelitian lanjutan yang lebih mendalam mengenai pengetahuan lokaluntuk variabel lain

Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions

Methods for site-specific modification of proteins should be quantitative and versatile with respect to the nature and size of the biological or chemical targets involved. They should require minimal modification of the target, and the underlying reactions should be completed in a reasonable amount of time under physiological conditions. Sortase-mediated transpeptidation reactions meet these criteria and are compatible with other labeling methods. Here we describe the expression and purification conditions for two sortase A enzymes that have different recognition sequences. We also provide a protocol that allows the functionalization of any given protein at its C terminus, or, for select proteins, at an internal site. The target protein is engineered with a sortase-recognition motif (LPXTG) at the place where modification is desired. Upon recognition, sortase cleaves the protein between the threonine and glycine residues, facilitating the attachment of an exogenously added oligoglycine peptide modified with the functional group of choice (e.g., fluorophore, biotin, protein or lipid). Expression and purification of sortase takes ∼3 d, and sortase-mediated reactions take only a few minutes, but reaction times can be extended to increase yields.National Institutes of Health (U.S.) (Grant RO1 AI08787

Forward Physics at the LHC (Elba 2010)

The papers review the main theoretical and experimental aspects of the Forward Physics at the Large Hadron Collider

Site-specific protein modification using immobilized sortase in batch and continuous-flow systems

Transpeptidation catalyzed by ​sortase A allows the preparation of proteins that are site-specifically and homogeneously modified with a wide variety of functional groups, such as fluorophores, PEG moieties, lipids, glycans, bio-orthogonal reactive groups and affinity handles. This protocol describes immobilization of ​sortase A on a solid support (Sepharose beads). Immobilization of ​sortase A simplifies downstream purification of a protein of interest after labeling of its N or C terminus. Smaller batch and larger-scale continuous-flow reactions require only a limited amount of enzyme. The immobilized enzyme can be reused for multiple cycles of protein modification reactions. The described protocol also works with a Ca²⁺-independent variant of ​sortase A with increased catalytic activity. This heptamutant variant of ​sortase A (7M) was generated by combining previously published mutations, and this immobilized enzyme can be used for the modification of calcium-senstive substrates or in instances in which low temperatures are needed. Preparation of immobilized ​sortase A takes 1–2 d. Batch reactions take 3–12 h and flow reactions proceed at 0.5 ml h⁻¹, depending on the geometry of the reactor used.United States. National Institutes of Health (RO1 AI087879

Diamond detectors for the TOTEM timing upgrade

This paper describes the design and the performance of the timing detector developed by the TOTEM Collaboration for the Roman Pots (RPs) to measure the Time-Of-Flight (TOF) of the protons produced in central diffractive interactions at the LHC. The measurement of the TOF of the protons allows the determination of the longitudinal position of the proton interaction vertex and its association with one of the vertices reconstructed by the CMS detectors. The TOF detector is based on single crystal Chemical Vapor Deposition (scCVD) diamond plates and is designed to measure the protons TOF with about 50 ps time precision. This upgrade to the TOTEM apparatus will be used in the LHC run 2 and will tag the central diffractive events up to an interaction pileup of about 1. A dedicated fast and low noise electronics for the signal amplification has been developed. The digitization of the diamond signal is performed by sampling the waveform. After introducing the physics studies that will most profit from the addition of these new detectors, we discuss in detail the optimization and the performance of the first TOF detector installed in the LHC in November 2015.Peer reviewe

Dietary Supplements and Sports Performance: Herbals

This is the fourth in a series of six articles to discuss the major classes of dietary supplements (vitamins; minerals; amino acids; herbs or botanicals; metabolites, constituents/extracts, or combinations). The major focus is on efficacy of such dietary supplements to enhance exercise or sport performance

Elastic differential cross-section d sigma/dt at root s=2.76 TeV and implications on the existence of a colourless C-odd three-gluon compound state

The proton-proton elastic differential cross sectvion d sigma/dt has been measured by the TOTEM experiment at root s = 2.76 TeV energy with beta* = 11 m beam optics. The Roman Pots were inserted to 13 times the transverse beam size from the beam, which allowed tomeasure the differential cross-section of elastic scattering in a range of the squared four-momentum transfer (vertical bar t vertical bar) from 0.36 to 0.74 GeV2. The differential cross-section can be described with an exponential in the vertical bar t vertical bar-range between 0.36 and 0.54 GeV2, followed by a diffractive minimum (dip) at vertical bar t(dip)vertical bar = (0.61 +/- 0.03) GeV2 and a subsequent maximum (bump). The ratio of the ds/dt at the bump and at the dip is 1.7 +/- 0.2. When compared to proton-antiproton measurement of the D0 experiment at root s = 1.96 TeV, a significant difference can be observed. Under the condition that the effects due to the energy difference between TOTEM and D0 can be neglected, the result provides evidence for the exchange of a colourless C-odd three-gluon compound state in the t-channel of the proton-proton and proton-antiproton elastic scattering.Peer reviewe