Calibration Model of %Titratable Acidity (Citric Acid) for Intact Tomato by Transmittance SW-NIR Spectroscopy

The acidity (citric acid) is one of the chemical contents that can refer to the internal quality and the maturity index of tomato. The titratable acidity (%TA) can be predicted by a non-destructive method prediction by using the transmittance short wavelength (SW-NIR). Spectroscopy in the wavelength range between 665-955 nm. The set of 167 tomato samples divided into groups of 117 tomatoes sample for training set and 50 tomatoes sample for test set were used to establish the calibration model to predict and measure %TA by partial least squares regression (PLSR) technique. The spectra were pretreated with MSC pretreatment and it gave the optimal result for calibration model as (R = 0.92, RMSEC = 0.03%) and this model obtained high accuracy result to use for %TA prediction in test set as (R = 0.81, RMSEP = 0.05%). From the result of prediction in test set shown that the transmittance SW-NIR spectroscopy technique can be used for a non-destructive method for %TA prediction of tomatoes

Genetic diversity analysis and subspecies classification of Thailand rice landraces using DNA markers

Genetic diversity among 126 rice accessions, including 110 Thai landraces and 16 varieties used as subspecies reference, were evaluated by three types of DNA markers, InDel (Insertion/Deletion), inter-simple sequence repeat (ISSR) and simple sequence repeat (SSR) markers. Twelve InDel primer pairs, based on DNA sequence polymorphism between ‘93-11’ (indica) and ‘Nipponbare’ (japonica), were used to identify subspecies of landraces. Most of the local rice samples had either ‘93-11’ alleles or ‘Nipponbare’ alleles. The scatter plotting of the principal component analysis (PCA) and dendrogram results based on InDel data could clearly classify landraces into two groups, indica and japonica. InDel and SSR markers showed the average polymorphic information content (PIC) values of 0.3707 and 0.6367, respectively. The dendrogram, based on combining InDel, ISSR and SSR data, could classify rice samples into five clusters at a cut-off genetic similarity value of about 0.70. The genetic similarity within landraces was low, indicating that Thai local rice samples have a great genetic diversity. The results of this experiment provide helpful data for rice germplasm management in breeding program.Key word: Rice, genetic diversity, DNA markers, simple sequence repeat (SSR), inter-simple sequence repeat (ISSR), insertion/deletion (InDel)

Calibration Model of %Titratable Acidity (Citric Acid) for Intact Tomato by Transmittance SW-NIR Spectroscopy

The acidity (citric acid) is one of the chemical contents that can refer to the internal quality and the maturity index of tomato. The titratable acidity (%TA) can be predicted by a non-destructive method prediction by using the transmittance short wavelength (SW-NIR). Spectroscopy in the wavelength range between 665-955 nm. The set of 167 tomato samples divided into groups of 117 tomatoes sample for training set and 50 tomatoes sample for test set were used to establish the calibration model to predict and measure %TA by partial least squares regression (PLSR) technique. The spectra were pretreated with MSC pretreatment and it gave the optimal result for calibration model as (R = 0.92, RMSEC = 0.03%) and this model obtained high accuracy result to use for %TA prediction in test set as (R = 0.81, RMSEP = 0.05%). From the result of prediction in test set shown that the transmittance SW-NIR spectroscopy technique can be used for a non-destructive method for %TA prediction of tomatoes

Effect of niclosamide on the tegumental surface of Haplorchis taichui using scanning electron microscopy

The effect of niclosamide on the tegument of adult Haplorchis taichui (Trematoda: Heterophyidae) exposed in vitro was observed by scanning electron microscope. Adult worms were incubated in Tyrode's solution containing 0.01, 0.1, 1.0, and 10 microg ml(-1) of niclosamide for 30 min, 1, 6, 12 and 24 h. Control groups were incubated in Tyrode's solution without niclosamide and worms remained active until 24 h. In 0.01 microg ml(-1) of niclosamide, worms showed slightly active movements up to 1 h after incubation, while in 0.1 microg ml(-1) solution a few worms showed only slightly active movements after 30 min. Tegumental changes were determined by scanning electron microscopy. Swelling and blebbing of the tegument were observed on both ventral and dorsal sides. After longer periods, extensive swelling and blebbing of the tegument became more severe and there was a loss of the apical plasma membrane in some regions. Empty spine sockets occurred, and small perforations penetrated the basal lamina, followed by some lesions. Destruction of both surfaces was more pronounced on the posterior compared with the anterior regions

การออกแบบชุดลำลองสตรีในสไตล์มินิมอลโดยใช้ผ้าฝ้ายย้อมสีจากเปลือกไม้เคี่ยม

วิทยานิพนธ์ (คศ.ม.) -- มหาวิทยาลัยเทคโนโลยีราชมงคลพระนคร, 2561The purposes of the study “The Design of Lady Casual wear in Minimal Style from Resak died cotton cloth” were 1) to study design of Lady Casual wear in Minimal Style from Resak died cotton cloth. 2) to study consumer satisfaction with Lady Casual wear in Minimal Style from Resak died cotton cloth. Minimal Art Concept was used to guideline The Design of Lady Casual wear. The Design of Lady Casual wear was composed of 18 clothing sets, 6 types. The five Fashion Design Specialist by Purposive Sampling chose one from six sets. The researcher sewed 6 clothing sets which were selected and after that the 6 sets were used to find out the satisfaction of female students at Home Economics Rajamangala University of Technology Phra Nakhon. A sample was selected from accidental sampling. Data was analyzed by Percentage, Compensation and Standard Deviation. The results finding of the consumer satisfaction with Lady Casual wear in Minimal Style from Resak died cotton cloth showed that target groups are satisfied at a high level. The first is the shirt and skirt type (average 4.42).The second is the Overalls type (average 4.36). The third is the Blouses and shorts type (average 4.35). The fourth is the Blouses and trousers type (average 4.21). The fifth is the Coat type (average 4.10) and The Last is The Dress type (average 3.99).Rajamangala University of Technology Phra Nakho

Calibration Model of %Titratable Acidity (Citric Acid) for Intact Tomato by Transmittance SW-NIR Spectroscopy

The acidity (citric acid) is one of the chemical contents that can refer to the internal quality and the maturity index of tomato. The titratable acidity (%TA) can be predicted by a non-destructive method prediction by using the transmittance short wavelength (SW-NIR). Spectroscopy in the wavelength range between 665-955 nm. The set of 167 tomato samples divided into groups of 117 tomatoes sample for training set and 50 tomatoes sample for test set were used to establish the calibration model to predict and measure %TA by partial least squares regression (PLSR) technique. The spectra were pretreated with MSC pretreatment and it gave the optimal result for calibration model as (R = 0.92, RMSEC = 0.03%) and this model obtained high accuracy result to use for %TA prediction in test set as (R = 0.81, RMSEP = 0.05%). From the result of prediction in test set shown that the transmittance SW-NIR spectroscopy technique can be used for a non-destructive method for %TA prediction of tomatoes

Calibration Model of %Titratable Acidity (Citric Acid) for Intact Tomato by Transmittance SW-NIR Spectroscopy

The acidity (citric acid) is one of the chemical contents that can refer to the internal quality and the maturity index of tomato. The titratable acidity (%TA) can be predicted by a non-destructive method prediction by using the transmittance short wavelength (SW-NIR). Spectroscopy in the wavelength range between 665-955 nm. The set of 167 tomato samples divided into groups of 117 tomatoes sample for training set and 50 tomatoes sample for test set were used to establish the calibration model to predict and measure %TA by partial least squares regression (PLSR) technique. The spectra were pretreated with MSC pretreatment and it gave the optimal result for calibration model as (R = 0.92, RMSEC = 0.03%) and this model obtained high accuracy result to use for %TA prediction in test set as (R = 0.81, RMSEP = 0.05%). From the result of prediction in test set shown that the transmittance SW-NIR spectroscopy technique can be used for a non-destructive method for %TA prediction of tomatoes

Association of non-synonymous SNPs of <i>OPN</i> gene with litter size traits in pigs

Osteopontin (<i>OPN</i>) gene is a secreted phosphoprotein which appears to play a key function in the conceptus implantation, placentation and maintenance of pregnancy in pigs. The objectives of this study were to verify the non-synonymous single nucleotide polymorphisms (SNPs) and their association with litter size traits in commercial Thai Large White pigs. A total of 320 Thai Large White sows were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Three SNPs at c.425G> A, c.573T> C and c.881C> T revealed amino acid exchange rates of p.110Ala> Thr, p.159Val> Ala and p.262Pro> Ser, respectively, and were then segregated. These three SNPs were significantly associated with total number born (TNB) and number born alive (NBA) traits. No polymorphisms of the two SNP markers (c.278A> G and c.452T> G) were observed in this study. Moreover, the SNPs at c.425G> A and c.573T> C were found to be in strong linkage disequilibrium. The association of <i>OPN</i> with litter size emphasizes the importance of porcine <i>OPN</i> as a candidate gene for reproductive traits in pig breeding

Identification and characterization of novel single nucleotide polymorphism markers for fat deposition in muscle tissue of pigs using amplified fragment length polymorphism

Objective This study was conducted to identify and evaluate the effective single nucleotide polymorphism (SNP) markers for fat deposition in the longissimus dorsi muscles of pigs using the amplified fragment length polymorphism (AFLP) approach. Methods Sixty-four selective primer combinations were used to identify the AFLP markers in the 20 highest- and 20 lowest-intramuscular fat (IMF) content phenotypes. Five AFLP fragments were converted into simple codominant SNP markers. These SNP markers were tested in terms of their association with IMF content and fatty acid (FA) composition traits in 620 commercially crossbred pigs. Results The SSC7 g.4937240C>G marker showed an association with IMF content (pA marker revealed an association with palmitoleic and ω9 FA levels (pT marker showed a significant association with IMF content and FA levels of palmitoleic, eicosenoic, arachidonic, monounsaturated fatty acids, and ω9 FA levels. However, no significant association of SSC8 g.47338181G>A was observed with any IMF and FA levels in this study. Conclusion Four SNP markers (SSC7 g.4937240C>G, SSC9 g.5496647_5496662insdel, SSC10 g.71225134G>A, and SSC17 g.61976696G>T) were found to be associated with IMF and/or FA content traits in commercially crossbred pigs. These findings provide evidence of the novel SNP markers as being potentially useful for selecting pigs with the desirable IMF content and FA composition

SURVEY OF HELMINTHS IN CLIMBING PERCH (ANABAS TESTUDINEUS) FROM SAN SAI DISTRICT, CHIANG MAI PROVINCE

Abstract. A helminthological study of Anabas testudineus was conducted. Fifty fish were collected from natural water resources in San Sai District, Chiang Mai Province, between July and September 2003. Seven species of helminths were recovered, as follows; one Monogenea species, Trianchoratus sp, was recovered at gill filaments, with a prevalence of 32%. In the intestine, the adult stage Acanthocephala, nematodes, and trematodes were found: Pallisentis sp (22%), Camallanus sp (11%), and Allocreadium sp (2%), respectively. Moreover, metacercarial stages of three species: Stellantchasmus falcatus (88%), Acanthostomum sp (78%), and Centrocestus caninus (70%), were examined in the body portion of the fish (gills, fins, scales, head, and muscles). Two species of metacercarial stage, Centrocestus caninus and Stellantchasmus falcatus, are helminths that can infect humans. plates, and then examined under a compound microscope. The intestine was dissected by needles and the presence of worms was determined using a stereomicroscope. The head and muscles were chopped and digested with crude extract solution of pineapple in a blender. Sequentially, the mixture was transferred into Erlenmeyer flasks, then incubated in a shaking water bath at 37˚C for 2 hours. The metacercariae were isolated using graded sieves and then rinsed with 0.85% sodium chloride solution. The encysted metacercariae were studied and identified with excysted metacercariae under a compound microscope. All of the worms were fixed in 5% formalin, stained with hematoxylin or borax carmine, dehydrated in alcohol series, and mounted with permount. Species identification was carried out by morphological examination, as described by RESULTS Out of 50 climbing perch (Anabas testudineus) collected from natural water sources in San Sai District, 49 were infected with 7 species of helminths. The species with the highest infection rate was Stellantchasmus falcatus (88%) while Acanthostomum sp, Centrocestus caninus, Camallanus sp, Trianchoratus sp, and Pallisentis sp were present in 78, 70, 66, 32, and 22%, respectively. The species with the lowest infection rate was Allocreadium sp (2%), as shown in The result revealed that S. falcatus showed the highest mean intensity, at 59.64 (2,982/50), while Acanthostomum sp and C. caninus were 41 (2,092/50) and 40.82 (2,041/50), respectively. In contrast, the adult stage of the trematode, Allocreadium sp, wa