Разработка стандартного образца состава молочной кислоты

A state standard sample (GSO) of lactic acid content to testify and control accuracy and precision of procedures for determining lactic acid weight concentration in food was developed. It goes to the making of graduation, calibration and adjustment of measuring devices. Metrological characteristics for standard sample of lactic acid content - inhomogeneity and instability errors - were determined.Разработан стандартный образец утвержденного типа (ГСО) состава кислоты молочной для аттестации и контроля точности методик измерений массовой доли кислоты молочной в кислоте молочной, а также пищевой продукции для градуировки средств измерений; для калибровки средств измерений при соответствии метрологических характеристик стандартного образца требованиям методик калибровки. При определении метрологических характеристик СО оценены характеристики неоднородности и нестабильности материала стандартного образца состава молочной кислоты

Development of a lactic acid standard sample

A state standard sample (GSO) of lactic acid content to testify and control accuracy and precision of procedures for determining lactic acid weight concentration in food was developed. It goes to the making of graduation, calibration and adjustment of measuring devices. Metrological characteristics for standard sample of lactic acid content - inhomogeneity and instability errors - were determined

Stability processes of moving invariant manifolds in uncertain impulsive differential-difference equations

summary:We present a result on the stability of moving invariant manifolds of nonlinear uncertain impulsive differential-difference equations. The result is obtained by means of piecewise continuous Lyapunov functions and a comparison principle

Evaluating an Ordered List of Recommended Physical Activities within Health Care System

Information and communication technologies make it possible to bridge the gap and time barriers in the flow of health information and knowledge, allowing every involved part in the health process to have access to the information. This approach provides the knowledge of the individual to contribute effectively to the improvement in human health. But also, helps the collective knowledge effectively to solve health problems on individual level. In this paper we are evaluating the algorithm that generates recommendation for users. We are using simulations on generic data to see how different types of activities are affecting the accuracy of the algorithm. On the basis of the performed activities and blood glucose measurements, our recommendation algorithm should determine list of activities that have bigger influence on the change of the blood glucose levels. Generic data for our simulations are based on modeling of food intake and physical activity influence over the blood glucose level

ANESTHESIA FOR MICROSURGICAL AUTOTRANSPLANTATION OF TOES ON THE WRIST IN CHILDREN WITH CONGENITAL AND POSTTRAUMATIC PATHOLOGY

Intraoperative combination of monolateral spinal anesthesia and prolonged brachial plexus block is evaluated in 65 children with autotransplantation of fingers from foot to hand. Variability of heart rhythm demonstrated autonomous stability in the main group. Dynamical study of skin temperature and dopplerographic evaluation demonstrated local sympatholysis in the blocked extremity. Significant improvement of circulation and quality of analgesia is revealed in case of prolonged perineural use of local anesthetics in the postoperative period. Described combination of intra- and postoperative treatment of such patients makes complex treatment more effective

Zofenopril in the treatment of patients with arterial hypertension and stable coronary heart disease: effects on oxidative stress and flowdependent vasodilatation

Aim. To compare the effects of ACE inhibitors zofenopril and perindopril on endothelial function and oxidative stress (OS) in patients with stable CHD and AH. Material and methods. In total, 40 patients with stable CHD (Functional Class II-III effort angina) and Stage 1-2 AH received zofenopril (7,5-30 mg/d; mean dose 18,6±8,8 mg/d; n=17) or perindopril (2-8 mg/d; mean dose 4,1±2,1 mg/d; n=23) for 12 weeks. At baseline and in the end of the study, all patients underwent reactive hyperemia test (RHT), to assess flow-dependent vasodilatation of brachial artery, and the measurement of OS parameters (malone dialdehyde, MDA, in low-density lipoproteins, MDALDL) and antioxidant parameters (superoxide dismutase (SOD) and glutathione peroxidase (GPO) activity in erythrocytes).Results. In both groups, a similar reduction in systolic and diastolic blood pressure levels was observed. In the zofenopril group, a significant elevation in brachial artery diameter increase during RHT, a significant increase in GPO activity, and some reduction in MDALDL levels were observed, which points to antioxidant system (AOS) activation and OS reduction. No similar changes of these parameters were observed in the perindopril group. Conclusion. In patients with stable CHD and AH, zofenopril, but not perindopril, reduced OS severity and increased AOS system activity, which was associated with improved endothelial-dependent vasodilatation

Reactions of glutathionylcobalamin with nitroxyl and its donor, Angeli’s salt

We investigated the reaction between glutathionylcobalamin (GSCbl), a tight complex of cobalamin with glutathione (GSH), and nitroxyl (HNO) produced upon decomposition of Angeli’s salt using ultraviolet-visible spectroscopy and 1H NMR. The reaction in neutral and alkaline media leads to formation of nitrosylcobalamin (NOCbl). Simulation of the kinetic traces using the ChemMech program package suggested that the mechanism of the process involves complexation between HNO and GSCbl and further decomposition of the complex into the products. The complex participates in an acid-base equilibrium (pKa = 8.9), and the deprotonated form decomposes to NOCbl more rapidly than the protonated species. At pH 7.4, the reaction of HNO with GSCbl proceeds ca.103-times more slowly than with free GSH. GSCbl can react directly with the monoprotonated anion of Angeli’s salt (HN2O3−) to produce nitrocobalamin (NO2Cbl). The reaction between GSCbl and HN2O3− is insignificant in an alkaline medium and is accelerated upon acidification of the medium. The critical step of the process is the complexation between GSCbl and HN2O3−. The produced complex is involved in an acid-base equilibrium (pKa = 8.1): the protonated form can be transformed to NO2Cbl and GSNHO−, whereas the deprotonated species decompose to initial reactants.</p