Preliminary Results of Aerodynamic Heating Studies on the X-15 Airplane

Aerodynamic heating analysis of X-15 aircraft in fligh

How Well Can You Tailor the Charge of Lipid Vesicles?

Knowledge and control of surface charge or potential is important for tailoring colloidal interactions. In this work, we compare widely used zeta potential (ζ) measurements of charged lipid vesicle surface potential to direct measurements using the surface force apparatus (SFA). Our measurements show good agreement between the two techniques. On varying the fraction of anionic lipids dimyristoylphosphatidylserine (DMPS) or dimyristoylphosphatidylglycerol (DMPG) mixed with zwitterionic dimyristoylphosphatidylcholine (DMPC) from 0 to 100 mol % we observed a near-linear increase in membrane surface charge or potential up to 20-30 mol % charged lipids beyond which charge saturation occurred in physiological (high) salt conditions. Similarly, in low salt concentrations, a linear increase in charge/potential was found but only up to ∼5-10 mol % charged lipids beyond which the surface charge or potential leveled off. While a lower degree of ionization is expected due to the lower dielectric constant (ε ∼ 4) of the lipid acyl chain environment, increasing intramembrane electrostatic repulsion between neighboring charged lipid head groups at higher charge loading contributes to charge suppression. Measured potentials in physiological salt solutions were consistent with predictions using the Gouy-Chapman-Stern-Grahame (GCSG) model of the electrical double layer with Langmuir binding of counterions, but in low salt conditions, the model significantly overestimated the surface charge/potential. The much lower ionization in low salt (maximum ∼1-2% of total lipids ionized) instead was consistent with counterion condensation at the bilayer surface which limited the charge that could be obtained. The strong interplay between membrane composition, lipid headgroup ionization, electrolyte concentration, and solution pH complicates exact prediction and tuning of membrane surface charge for applications. However, the theoretical frameworks used here can provide guidelines to understand this interplay and establish a range of achievable potentials for a system and predict the response to triggers like pH and salt concentration changes

Preweaning and Postweaning Performance of Crossbred Calves--0, 1 or 2 Ralgro Implants

The preweaning performance of 221 steer and heifer calves given differing numbers of Ralgro implants was evaluated. The steer and heifer calves given one implant had an additional 23 and 33 lb weaning weight compared to those receiving no implant. No additional response was shown by steer calves receiving a second implant 100 days after the first was given. Average daily gains during the 105-day growing period were not significantly faster than those receiving two implants during the 103-day finishing phase. This work supports other studies indicating that implanting calves at weaning time is an economical management practice. The work further indicates to feeders that calfhood implants have little or no effect on subsequent performance in the feedlot

Effect of Length of Feeding Period on Performance of British and Exotic Crossbred Yearling Heifers

Seventy-two heifers were fed for 47, 61 and 75 days to determine the optimum feeding period for exotic cross and black baldy heifers. Average daily gains and feed conversions of the heifers were similar among treatments. Carcass weight, quality grade and yield grade increased with time on feed. Fat thickness, quality grade and yield grade increased faster for the black baldy heifers. Cost per pound of gain was similar for all treatments. The optimum weight at which to slaughter the black baldy yearling heifers appeared to be between 1000 and 1050 lb., both from a quality and economic view point. The optimum weight for slaughtering the exotic cross heifers was apparently not reached, as the last slaughter group was still gaining rapidly and efficiently with little increase in condition. The limited research conducted in this study indicates that a producer with mixed lots of cattle needs to be aware of the weight at which different types of cattle reach optimum condition. The feeder has greater flexibility in marketing the larger framed exotic cross type cattle as compared to the relatively smaller framed English breeds

Feeding Value of Pro-Sil Treated High-Moisture Ground Ear Corn with Two Groups of Crossbred Heifers

Pro-Sil, an ammonia-molasses-mineral suspension, has been shown to be a highly effective commercial additive for corn silage in studies at SDSU and other midwest universities. When added to corn silage at the time of ensiling, this product stimulates beneficial fermentation and increases the crude protein content of the silage, thereby reducing or eliminating the need for protein supplementation at the time of feeding. While Pro-Sil has been thoroughly tested with whole plant corn silage, no research has been conducted with ensiled high-moisture ear corn (HMEC). Since the crude protein content of HMEC is low compared to the needs of feedlot cattle, research is needed to evaluate economical methods of boosting the level of this critical nutrient. Thus, the major objective of this initial study was to compare the feedlot performance of cattle fed Pro-Sil treated HMEC or regular HMEC mixed with either a control or a urea-based protein supplement at the time of feeding. Both types of HMEC were ensiled in a plastic Silopress bag to examine the effectiveness of this storage method with HMEC. The relative performance of exotic and British crossbred yearling heifers was also studied in this trial

Vpu-mediated tetherin antagonism of ongoing HIV-1 infection in CD4+ T-cells is not directly related to the extent of tetherin cell surface downmodulation

AbstractTetherin is a host cell restriction factor that acts against HIV-1 and other enveloped viruses. The antiviral activity of tetherin is antagonized by the HIV-1 protein Vpu, that downregulates tetherin from the cell surface.Here, we report the specific detection of cell surface tetherin levels in primary activated CD4+ T-cells and in CD4+ T-cell lines. Differences were observed regarding tetherin cell surface expression, Vpu-mediated tetherin downmodulation and promotion of virus release. However, Vpu expression in all T-cell lines resulted in a 2-fold increase in numbers of infected cells after three days. This implies a Vpu-mediated effect in ongoing infection and possibly in cell-to-cell viral spread that is independent of the extent of Vpu-mediated tetherin cell surface downmodulation. Endogenous cell surface tetherin levels in T-cell lines were also downmodulated following infection with Vpu-deleted virus, suggesting an additional Vpu-independent mechanism of tetherin cell surface downmodulation following HIV-1 infection in T-cell lines

Tetherin and Its Viral Antagonists

Restriction factors comprise an important layer of host defense to fight against viral infection. Some restriction factors are constitutively expressed whereas the majority is induced by interferon to elicit innate immunity. In addition to a number of well-characterized interferon-inducible antiviral factors such as RNaseL/OAS, ISG15, Mx, PKR, and ADAR, tetherin (BST-2/CD317/HM1.24) was recently discovered to block the release of enveloped viruses from the cell surface, which is regarded as a novel antiviral mechanism induced by interferon. Here, we briefly review the history of tetherin discovery, discuss how tetherin blocks virus production, and highlight the viral countermeasures to evade tetherin restriction

On the theory of cavities with point-like perturbations. Part I: General theory

The theoretical interpretation of measurements of "wavefunctions" and spectra in electromagnetic cavities excited by antennas is considered. Assuming that the characteristic wavelength of the field inside the cavity is much larger than the radius of the antenna, we describe antennas as "point-like perturbations". This approach strongly simplifies the problem reducing the whole information on the antenna to four effective constants. In the framework of this approach we overcame the divergency of series of the phenomenological scattering theory and justify assumptions lying at the heart of "wavefunction measurements". This selfconsistent approach allowed us to go beyond the one-pole approximation, in particular, to treat the experiments with degenerated states. The central idea of the approach is to introduce ``renormalized'' Green function, which contains the information on boundary reflections and has no singularity inside the cavity.Comment: 23 pages, 6 figure