Physical therapy methods in the treatment and rehabilitation of cancer patients

The results of the effective use of magnetic laser therapy in the treatment and rehabilitation of cancer patients were presented. The effect of magnetic-laser therapy in the treatment of radiation-induced reactions in the patients with head and neck cancer and in the patients with breast cancer was analyzed. High efficiency of lymphedema and lymphorrhea treatment in the postoperative period in the patients with breast cancer was proved. The results of rehabilitation of the patients with gastric cancer after surgical treatment were presented. These data indicate a high effectiveness of different physical methods of treatment and rehabilitation of cancer patients

Endothelial Microvascular Dysfunction and Its Relationship with Haptoglobin Levels in Patients with Different Phenotypes of Chronic Heart Failure

Aim. To study the relationship between the level of haptoglobin and the main indicators of microcirculation (MC) in patients with different phenotypes of chronic heart failure (CHF).Materials and methods. Patients with different phenotypes of functional class II-IV chronic heart failure according to NYHA (n=80) underwent a general clinical examination, determination of the serum haptoglobin level by enzyme-linked immunosorbent assay, as well as an assessment of the MC state on the medial surface of the upper third of the leg by laser Doppler flowmetry (LDF).Results. Patients with CHF included patients with preserved left ventricular ejection fraction (HFpEF; n=27, intermediate ejection fraction (HFmrEF; n=25) and reduced ejection fraction (HFrEF; n=28). The median value of haptoglobin in the HFpEF group was 1387.6 [ 747.5; 1946.9] mg/l, in the HFmrEF group was 1583.4 [818.9; 2201.4] mg/l, in the HFrEF group was 968.5 [509.5; 1324.4] mg/l. Correlation analysis revealed statistically significant relationships between haptoglobin and the amplitudes of the endothelial frequency range (Ae) in the groups of HFmrEF (r=-0.628, 95% confidence interval [CI] -0.256; -0.825, p=0.003) and HFrEF (r=-0.503, 95% CI -0.089; -0.803, p=0.02). A negative relationship between the haptoglobin level and Kv and σ was revealed, as well as a formula for calculating the value of haptoglobin was obtained, which is predicted on the basis of the amplitude index of the endothelial frequency range: [haptoglobin]=1787-(4053×Ae).Conclusion. The multifactorial effect of haptoglobin is realized in the central and peripheral mechanisms of MC regulation. Low values of haptoglobin in blood plasma should be considered as a potential marker for the development of complications and used in a comprehensive assessment of the state of patients with CHF. Evaluation of the diagnostic and prognostic significance of haptoglobin, especially in patients with HFmrEF, requires further study

REVIEW OF ENERGY-EFFICIENT HOUSES IN NORTHERN EUROPE AND CANADA

The theme of energy efficiency and conservation relevant today as ever. Worldwide, the reduction of energy consumption is one of the most important tasks. Passive house is a fairly new standard for residential buildings. Thanks to the insulation and sealing of building envelope, heating costs are minimized and it is not necessary in the usual heating systems. This article discusses options for passive house, what have passed successful approbation in Northern Europe and Canada, for Russia.Тема энергоэффективности и энергосбережения актуальна сегодня как никогда. Во всем мире снижение потребления энергоресурсов является одной из важнейших задач. Пассивные дома – это достаточно новый стандарт для жилых строений. Благодаря утеплению и герметизации оболочки здания затраты на отопление в нем минимизированы и нет необходимости в привычных системах отопления. В данной статье рассматриваются варианты энергопассивных домов, прошедших успешную апробацию в Северных странах Европы и Канады, для России

Umbilical cord blood as a promising source of NK cells for immunotherapy

Currently, a large number of studies on genetic modification of cord blood NK cells (UCB-NK) are carried out at both clinical and preclinical levels. Immunotherapy based on UCB-NK cells has great potential for antitumor therapy. However, despite having known several advantages over peripheral blood NK cells (PB- NK), including a high concentration in cord blood and low virulence rate, UCB-NK cells are predominantly characterized in the scientific literature as immature and low-functioning NK cells. In this work, we studied the phenotypic characteristics of UCB-NK cells and the possibility of stimulatory compensation of the decreased functional activity of UCB-NK cells. Our studies revealed UCB-NK cells can be characterized as poorly differentiated and weakly activated cells with high level of inhibitory receptor NKG2A and low level of activating receptor NKG2C and HLA-DR, accordingly with the literature data. Two types of stimuli were chosen to stimulate freshly isolated UCB-NK cells: 1) 100 units of IL-2; 2) combinations of 100 units IL-2 and K-562 feeder cells expressing membrane-bound IL-21 (K562-mbIL21). It was shown the degranulation (LAMP-1) and proliferative activity was higher than for parallel cultured ex vivo PB-NK cells under the same conditions for UCB-NK cells stimulated for 7 days with IL-2 + K562-mbIL21. Moreover, stimulation in the way of IL-2 + K562-mbIL21 seemed to be a more perspective way to obtain a large number of proliferatively active UCB-NK cells compared to stimulation with IL-2 only. Since genetic modification of NK cells is a promising way to improve the antitumor properties of NK cells, retroviral transduction procedure was performed to study of the stimulated UCB-NK cells. UCB-NK cells stimulated with IL-2 + K562-mbIL21 were transduced on day 8 of cultivation. In this study, we used targeted overexpression of the adaptor molecule DAP12, which is involved in the signaling of activating NK cell receptors. PB-NK cells and UCB-NK cells were transduced under the equal experimental conditions in same volume of viral particles. As a result, the transduction efficiency was found to be more than 4-fold higher for UCB-NK cells compared to PB-NK cells. Thus, UCB-NK cells appear to be a promising tool for further research in cancer immunotherapy

The possibilities of adapting the re-processing protocol in the practice of the ThinPrep Pap test usage

The method of liquid cytology is a newer diagnostic test compared to the traditional cytological examination of scrapings from the cervix and allows one to standardize the morphological examination and reduce the number of unsatisfactory samples. One of the factors determining the adequacy of the Pap test is the number of squamous epithelial cells in a sample. The abundance of blood elements, mucus, lubricant, or inflammatory elements may affect the informativeness of the sample. In the practice of the ThinPrep Pap test usage, when receiving an inadequate sample, a protocol for re-processing the contents of the vial is used, in order to neutralize the possible influence of such impurities on the final result. Here, we present a review on the methods of the re-processing protocol application and our own experience of adapting the protocol in the practice of a clinical diagnostic laboratory

КОМПЬЮТЕРНАЯ ДИАГНОСТИКА В ЛЕЧЕНИИ ПАТОЛОГИЙ РИНОСИНУСОТУБАРНОЙ ЗОНЫ

This article presents the capabilities of computer tomography in the diagnosis and treatment algorithm combine pathology (acute sinusitis, acute otitis media, Eustachian tube dysfunction).В статье представлены возможности компьютерной томографии в диагностике и алгоритм лечения патологии риносинусотубарной зоны

Design of hepatotoxicity and cardiotoxicity experimental evaluation of leflunomid in rabbits

The article deals the experiment methodology on rabbits in order to assess hepatotoxicity of leflunomide during the intake of essential phospholipids. A model of drug administration was chosen, a concept of manipulations for evaluation of hepatotoxicity was developed, which includes blood sampling, weighing, liver biopsy as well as cardiotoxicity – electrocardiography.В статье представлена методология проведения эксперимента на кроликах по оценке гепатотоксичности лефлуномида на фоне приёма эссенциальных фосфолипидов. Подобрана модель введения препарата, разработана концепция манипуляций для оценки гепатотоксичности: забор крови, взвешивание, биопсия печени, а также кардиотоксичности – снятие электрокардиограммы