Does Combined Therapy of Curcumin and Epigallocatechin Gallate have a synergistic Neuroprotective Effect Aainst Spinal Cord Injury?

Systematic inflammatory response after spinal cord injury (SCI) is one of the factors leading to lesion development and a profound degree of functional loss. Anti-inflammatory compounds, such as curcumin and epigallocatechin gallate (EGCG) are known for their neuroprotective effects. In this study, we investigated the effect of combined therapy of curcumin and EGCG in a rat model of acute SCI induced by balloon compression. Immediately after SCI, rats received curcumin, EGCG, curcumin + EGCG or saline [daily intraperitoneal doses (curcumin, 6 mg/kg; EGCG 17 mg/kg)] and weekly intramuscular doses (curcumin, 60 mg/kg; EGCG 17 mg/kg)] for 28 days. Rats were evaluated using behavioral tests (the Basso, Beattie, and Bresnahan (BBB) open-field locomotor test, flat beam test). Spinal cord tissue was analyzed using histological methods (Luxol Blue-cresyl violet staining) and immunohistochemistry (anti-glial fibrillary acidic protein, anti-growth associated protein 43). Cytokine levels (interleukin-1beta, interleukin-4, interleukin-2, interleukin-6, macrophage inflammatory protein 1-alpha, and RANTES) were measured using Luminex assay. Quantitative polymerase chain reaction was performed to determine the relative expression of genes (Sort1, Fgf2, Irf5, Mrc1, Olig2, Casp3, Gap43, Gfap, Vegf, NfkappaB, Cntf) related to regenerative processes in injured spinal cord. We found that all treatments displayed significant behavioral recovery, with no obvious synergistic effect after combined therapy of curcumin and ECGC. Curcumin and EGCG alone or in combination increased axonal sprouting, decreased glial scar formation, and altered the levels of macrophage inflammatory protein 1-alpha, interleukin-1beta, interleukin-4 and interleukin-6 cytokines. These results imply that although the expected synergistic response of this combined therapy was less obvious, aspects of tissue regeneration and immune responses in severe SCI were evident

Heterogenni a anizotropni difuze v extracelularnim prostoru nervove tkane.

Available from STL Prague, CZ / NTK - National Technical LibrarySIGLECZCzech Republi

Trehalose in ophthalmology

Trehalose, a disaccharide of glucose, is a naturally occurring nontoxic and nonreducing bioactive sugar. Trehalose is synthetized by many organisms when cells are exposed to stressful conditions, including dehydration, heat, oxidation, hypoxia or even anoxia. Although trehalose is not synthesized by mammalian cells, it has recently been demonstrated to have a number of important properties that indicate its utility in humans. Trehalose enables wound healing by protecting cells, especially cell membranes, from oxidative injury and dessication. When the injured cornea is treated with trehalose, corneal inflammation, scar formation and corneal neovascularization are suppressed. In dry eye disease, trehalose decreased cell apoptosis and reduced oxidative, inflammatory and proteolytic activity at the ocular surface. In UVB irradiated cornea, trehalose suppressed photodamage evoked by UVB rays. It decreased the intracorneal inflammation and reduced corneal neovascularization. Trehalose prevented postoperative fibrous scar formation after ocular surgery, such as glaucoma filtration surgery. The non-toxicity of trehalose allows its administration in humans for extended periods and enables its use in various disease states

Retraction Note: Therapeutic effect of molecular hydrogen in corneal UVB-induced oxidative stress and corneal photodamage (Scientific Reports, (2017), 7, 1, (18017), 10.1038/s41598-017-18334-6)

The Editors have retracted this Article. An institutional investigation found evidence that the H2 condition for Figure 3B has been duplicated from Figure 5C of an article previously published by Cejka et al. (2016)1 and the day 4 H2 condition for Figure 4A has been duplicated from Figure 3 of an article published later by Cejka et al. (2020)2. The authors have not been able to provide all the relevant raw data on request. The Editors therefore no longer have confidence in the accuracy of the reported data and the conclusions of the Article. Sarka Kubinova agrees with the retraction and its wording. Cestmir Cejka, Jan Kossl, Barbora Hermankova, Vladimir Holan, John H. Zhang, and Jitka Cejkova disagree with the retraction

Analýza chondroitin / dermatan sulfát disacharidů pomocí vysokoúčinné kapalinové chromatografie

Chondroitin sulphates belong to a group of naturally occurring glycosaminoglycans and play a role in many physiological processes including ageing and the effects of various diseases. Research into chondroitin sulphates has found that the most important analytes are 4- and 6-sulphated disaccharides. We developed an HPLC method for the separation and quantification of underivatized chondroitin/dermatan sulphates-unsaturated disaccharides (4- and 6-sulphated disaccharides). This method is based on the separation of disaccharides by amido as well as amino columns under acidic conditions. These columns enabled the successful separation of 4- and 6-sulphated disaccharides using 50 (amido column) and 25 mmol/L (amino column) phosphate buffer, pH 4.25 (detection at 230 nm), at retention times of less than 10 min. The limit of quantification was 0.5 mu g/mL. The applicability of this method was demonstrated through analysis of unsaturated disaccharides produced from the enzymatic digestion of chondroitin/dermatan sulphates of the solubilized extracellular matrix produced from porcine urinary bladder and human umbilical cord.Chondroitin sulfáty patří do skupiny přirozeně se vyskytujících glykosaminoglykanů a hrají roli v mnoha fyziologických procesech včetně stárnutí a vlivu různých nemocí. Výzkum chondroitin sulfátů zjistil, že nejdůležitějšími analyty jsou 4- a 6-sulfatované disacharidy. Vyvinuli jsme metodu HPLC pro separaci a kvantifikaci nederivatizovaných chondroitin / dermatan sulfáty-nenasycených disacharidů (4- a 6-sulfatované disacharidy). Tato metoda je založena na separaci disacharidů amidovými i amino kolonami v kyselém prostředí. Tyto kolony umožnily úspěšnou separaci 4- a 6-sulfatovaných disacharidů s použitím 50 (amidová kolona) a 25 mmol/l (aminová kolona) fosfátového pufru, pH 4,25 (detekce při 230 nm), při retenčních časech kratších než 10 minut. Mez kvantifikace byla 0,5 μg/ml. Použitelnost této metody byla prokázána analýzou nenasycených disacharidů produkovaných enzymatickým štěpením chondroitin / dermatan sulfátů solubilizované extracelulární matrice vyrobené z prasečího močového měchýře a lidské pupeční šňůry