Properties of quasi two-dimensional condensates in highly anisotropic traps

We theoretically investigate some of the observable properties of quasi two-dimensional condensates. Using a variational model based on a Gaussian-parabolic trial wavefunction we calculate chemical potential, condensate size in time-of-flight, release energy and collective excitation spectrum for varying trap geometries and atom numbers and find good agreement with recent published experimental results.Comment: 7 pages, 4 figure

Stem cell marker (Nanog) and Stat-3 signaling promote MicroRNA-21 expression and chemoresistance in hyaluronan/CD44-activated head and neck squamous cell carcinoma cells.

MicroRNAs are often associated with the pathogenesis of many cancers, including head and neck squamous cell carcinoma (HNSCC). In particular, microRNA-21 (miR-21) appears to have a critical role in tumor cell survival, chemoresistance and HNSCC progression. In this study, we investigated matrix hyaluronan (HA)-induced CD44 (a primary HA receptor) interaction with the stem cell markers, Nanog and Stat-3, in HNSCC cells (HSC-3 cells). Our results indicate that HA binding to CD44 promotes Nanog-Stat-3 (also tyrosine phosphorylated Stat-3) complex formation, nuclear translocation and transcriptional activation. Further analyses reveal that miR-21 is controlled by an upstream promoter containing Stat-3 binding site(s), while chromatin immunoprecipitation assays demonstrate that stimulation of miR-21 expression by HA/CD44 signaling is Nanog/Stat-3-dependent in HNSCC cells. This process results in a decrease of a tumor suppressor protein (PDCD4), and an upregulation of i nhibitors of the apoptosis family of proteins (IAPs) as well as chemoresistance in HSC-3 cells. Treatment of HSC-3 cells with Nanog- and/or Stat-3-specific small interfering RNAs effectively blocks HA-mediated Nanog-Stat-3 signaling events, abrogates miR-21 production and increases PDCD4 expression. Subsequently, this Nanog-Stat-3 signaling inhibition causes downregulation of survival protein (IAP) expression and enhancement of chemosensitivity. To further evaluate the role of miR-21 in tumor cell-specific functions, HSC-3 cells were also transfected with a specific anti-miR-21 inhibitor in order to silence miR-21 expression and block its target functions. Our results demonstrate that anti-miR-21 inhibitor not only upregulates PDCD4 expression but also decreases IAP expression and enhances chemosensitivity in HA-treated HNSCC cells. Together, these findings indicate that the HA-induced CD44 interaction with Nanog and Stat-3 has a pivotal role in miR-21 production leading to PDCD4 reduction, IAP upregulation and chemoresistance in HNSCC cells. This novel Nanog/Stat-3 signaling pathway-specific mechanism involved in miR-21 production is significant for the formation of future intervention strategies in the treatment of HA/CD44-activated HNSCC

Characterization of Cre recombinase activity for in vivo targeting of adipocyte precursor cells.

The increased incidence of obesity and metabolic disease underscores the importance of elucidating the biology of adipose tissue development. The recent discovery of cell surface markers for prospective identification of adipose precursor cells (APCs) in vivo will greatly facilitate these studies, yet tools for specifically targeting these cells in vivo have not been identified. Here, we survey three transgenic mouse lines, Fabp4-Cre, PdgfRα-Cre, and Prx1-Cre, precisely assessing Cre-mediated recombination in adipose stromal populations and mature tissues. Our data provide key insights into the utility of these tools to modulate gene expression in adipose tissues. In particular, Fabp4-Cre is not effective to target APCs, nor is its activity restricted to these cells. PdgfRα-Cre directs recombination in the vast majority of APCs, but also targets other populations. In contrast, adipose expression of Prx1-Cre is chiefly limited to subcutaneous inguinal APCs, which will be valuable for dissection of APC functions among adipose depots

Effects of stocking on genetics of wild brook trout populations

http://www.worldcat.org/oclc/1896394

Agriculture and riparian areas

Agriculture has historically been based in the subirrigated riparian ecosystems. Often the engineering and agricultural practices have altered the systems and many of the associated ecological processes. In the Western United States, the most common agricultural practices affecting riparian systems has been livestock grazing. Effects have been both positive and negative. Lack of management has deteriorated many of these systems. Current research has shown what types of management have been successful in allowing grazing by livestock to improve the grazing capacity

Implementation issues in product line scoping

Often product line engineering is treated similar to the waterfall model in traditional software engineering, i.e., the different phases (scoping, analysis, architecting, implementation) are treated as if they could be clearly separated and would follow each other in an ordered fashion. However, in practice strong interactions between the individual phases become apparent. In particular, how implementation is done has a strong impact on economic aspects of the project and thus how to adequately plan it. Hence, assessing these relationships adequately in the beginning has a strong impact on performing a product line project right. In this paper we present a framework that helps in exactly this task. It captures on an abstract level the relationships between scoping information and implementation aspects and thus allows to provide rough guidance on implementation aspects of the project. We will also discuss the application of our framework to a specific industrial project

Experimental observation of the 'Tilting Mode' of an array of vortices in a dilute Bose-Einstein Condensate

We have measured the precession frequency of a vortex lattice in a Bose-Einstein condensate of 87Rb atoms. The observed mode corresponds to a collective motion in which all the vortices in the array are tilted by a small angle with respect to the z-axis (the symmetry axis of the trapping potential) and synchronously rotate about this axis. This motion corresponds to excitation of a Kelvin wave along the core of each vortex and we have verified that it has the handedness expected for such helical waves, i.e. precession in the opposite sense to the rotational flow around the vortices. The experimental method used to excite this collective mode closely resembles that used to study the scissors mode and excitation of the scissors mode for a condensate containing a vortex array was used to determine the angular momentum of the system. Indeed, the collective tilting of the array that we have observed has previously been referred to as an `anomalous' scissors mode.Comment: 5 pages, 7 figures to be published in PR