Statistical Research of Expected Life Expectancy in the North Western Federal District: Panel analysis

In article possibility of application of statistical models of regression type for research of medico-demographic processes and an assessment of expected life expectancy depending on socio-economic factors for regions of the North Western federal district is considered

The safety and quality management in the production of brine cheeses

The article examines the implementation of the HACCP system at an operating enterprise. This system is a scientifically based system that allows to produce safe and high-quality products by identifying and controlling hazardous factors. An analysis of the existing safety management system at the plant was carried out, and necessary procedures and measures were proposed in connection with the implementation of a new product, brine cheese. To ensure the quality of the product, two new critical control points were proposed (CCP 2 – brine preparation; CCP 3 – storage of finished products), as well as one operating program (OPP 3 – packaging). The analysis of the zoning system at the enterprise was carried out, and it was recommended to make changes in this direction in accordance with the requirements of the HACCP. The proposed measures will ensure the production of high-quality pickled cheese, which in turn will ensure its competitiveness on the Ukrainian consumer market

Circadian rhythmicity in murine blood:Electrical effects of malaria infection and anemia

Circadian rhythms are biological adaptations to the day-night cycle, whereby cells adapt to changes in the external environment or internal physiology according to the time of day. Whilst many cellular clock mechanisms involve gene expression feedback mechanisms, clocks operate even where gene expression is absent. For example, red blood cells (RBCs) do not have capacity for gene expression, and instead possess an electrophysiological oscillator where cytosolic potassium plays a key role in timekeeping. We examined murine blood under normal conditions as well as in two perturbed states, malaria infection and induced anemia, to assess changes in baseline cellular electrophysiology and its implications for the electrophysiological oscillator. Blood samples were analyzed at 4-h intervals over 2 days by dielectrophoresis, and microscopic determination of parasitemia. We found that cytoplasmic conductivity (indicating the concentration of free ions in the cytoplasm and related to the membrane potential) exhibited circadian rhythmic behavior in all three cases (control, malaria and anemia). Compared to control samples, cytoplasm conductivity was decreased in the anemia group, whilst malaria-infected samples were in antiphase to control. Furthermore, we identified rhythmic behavior in membrane capacitance of malaria infected cells that was not replicated in the other samples. Finally, we reveal the historically famous rhythmicity of malaria parasite replication is in phase with cytoplasm conductivity. Our findings suggest the electrophysiological oscillator can impact on malaria parasite replication and/or is vulnerable to perturbation by rhythmic parasite activities

ГИПС В СИЛУРИЙСКИХ ОТЛОЖЕНИЯХ БЕЛАРУСИ: ИЗОТОПНЫЙ СОСТАВ СЕРЫ И ПРОИСХОЖДЕНИЕ

Gypsum was found in the Silurian deposits of Belarus for the first time. It composes nodules and fills cracks and fenestras in clayey-carbonate rocks revealed by the Davtiuny 3k borehole (Grodno area, Ostrovets region). With the use of the sulphur isotope data, it is shown that gypsum was formed at the diagenetic stage in the Silurian seawater concentrated under interground evaporation. The process was the result of a repeated superimposition of supratidal environment onto clayey- carbonate deposits formed in shallow subtidal and intratidal conditions.В силурийских отложениях Беларуси впервые выявлен гипс. Он слагает гнезда-желваки и выполняет трещины и фенестры в глинисто-карбонатных породах разреза скважины Давтюны 3к (Гродненская область, Островецкий район). С использованием данных об изотопном составе серы показано, что образование гипса протекало на стадии диагенеза в среде силурийской морской воды, сконцентрированной при внутригрунтовом испарении. Процесс инициировался многократным наложением надприливных обстановок на глинисто-карбонатные отложения, сформированные в мелководных подприливных и межприливных условиях

Casein Kinase 1 Underlies Temperature Compensation of Circadian Rhythms in Human Red Blood Cells

Temperature compensation and period determination by casein kinase 1 (CK1) are conserved features of eukaryotic circadian rhythms, whereas the clock gene transcription factors that facilitate daily gene expression rhythms differ between phylogenetic kingdoms. Human red blood cells (RBCs) exhibit temperature-compensated circadian rhythms, which, because RBCs lack nuclei, must occur in the absence of a circadian transcription-translation feedback loop. We tested whether period determination and temperature compensation are dependent on CKs in RBCs. As with nucleated cell types, broad-spectrum kinase inhibition with staurosporine lengthened the period of the RBC clock at 37°C, with more specific inhibition of CK1 and CK2 also eliciting robust changes in circadian period. Strikingly, inhibition of CK1 abolished temperature compensation and increased the Q10 for the period of oscillation in RBCs, similar to observations in nucleated cells. This indicates that CK1 activity is essential for circadian rhythms irrespective of the presence or absence of clock gene expression cycles

Terrestrial habitat requirements of nesting freshwater turtles

Because particular life history traits affect species vulnerability to development pressures, cross-species summaries of life history traits are useful for generating management guidelines. Conservation of aquatic turtles, many members of which are regionally or globally imperiled, requires knowing the extent of upland habitat used for nesting. Therefore, we compiled distances that nests and gravid females had been observed from wetlands. Based on records of \u3e 8000 nests and gravid female records compiled for 31 species in the United States and Canada, the distances that encompass 95% of nests vary dramatically among genera and populations, from just 8 m for Malaclemys to nearly 1400 m for Trachemys. Widths of core areas to encompass varying fractions of nesting populations (based on mean maxima across all genera) were estimated as: 50% coverage = 93 m, 75% = 154 m, 90% = 198 m, 95% = 232 m, 100% = 942 m. Approximately 6–98 m is required to encompass each consecutive 10% segment of a nesting population up to 90% coverage; thereafter, ca. 424 m is required to encompass the remaining 10%. Many genera require modest terrestrial areas (\u3c200 m zones) for 95% nest coverage (Actinemys, Apalone, Chelydra, Chrysemys, Clemmys, Glyptemys, Graptemys, Macrochelys, Malaclemys, Pseudemys, Sternotherus), whereas other genera require larger zones (Deirochelys, Emydoidea, Kinosternon, Trachemys). Our results represent planning targets for conserving sufficient areas of uplands around wetlands to ensure protection of turtle nesting sites, migrating adult female turtles, and dispersing turtle hatchlings

LEGAL REGULATION PROVIDING WITH SOCIAL SUPPORT AT A REDUCED FARECITIZENSINTHE REPUBLIC OF CRIMEA

The problems of creating the mechanism of rights` realization of certain categories citizens to price reductions in all types of urban, suburban and intercity communication within the range of the Republic of Crimea, its implementation, the prospects for improvement and development against the background integration of the Republic of Crimea in the economic, financial, legal system, and the system of state authorities of the Russian Federation are contemplated in this article

STATE SOCIAL ASSISTANCE ON THE BASIS OF THE SOCIAL CONTRACT REPUBLIC OF СRIMEA AS A MEASURE OF SOCIAL SUPPORT TO NEEDY FAMILIES IN THE CONTEXT OF THE CONCEPT OF THE STATE FAMILY POLICY IN THE RUSSIAN FEDERATION FOR THE PERIOD TILL 2025

The article is devoted to date topic — state support for low-income families and single residents of poor people by providing them with state social assistance on the basis of the social contract, which is in the priority actions to support, strengthen and protect the family, designed to enhance the quality of life for families and strengthening Social responsibility of citizens, encouraging their active actions to overcome difficult situations. The article analyzes existing in the subjects of the Russian Federation approaches to providing this type of assistance, the criteria for identifying the poor citizens, summarizes the experience of its practical provision, and make proposals for the improvement and development of a mechanism to provide, in particular — the accurate accounting of the actual average per capita incomes of poor families, to ensure the return to the budget amount of aid in the case of misuse, the unification of the conceptual apparatus of legal acts of subjects of the Russian Federation for ensuring a uniform approach to aid. Particular attention is paid to the prospects of state social assistance on the basis of the social contract in the Republic of Crimea with the January 1, 2016