2 research outputs found
Microbial Community Dynamics during a Harmful Chrysochromulina leadbeateri Bloom in Northern Norway
A harmful algal bloom occurred in late spring 2019 across multiple, interconnected fjords and bays in northern Norway. The event was caused by the haptophyte Chrysochromulina leadbeateri and led to severe fish mortality at several salmon aquaculture facilities. This study reports on the spatial and temporal succession dynamics of the holistic marine microbiome associated with this bloom by relating all detectable 18S and 16S rRNA gene amplicon sequence variants to the relative abundance of the C. leadbeateri focal taxon. A k-medoid clustering enabled inferences on how the causative focal taxon cobloomed with diverse groups of bacteria and microeukaryotes. These coblooming patterns showed high temporal variability and were distinct between two geographically separated time series stations during the regional harmful algal bloom. The distinct blooming patterns observed with respect to each station were poorly connected to environmental conditions, suggesting that other factors, such as biological interactions, may be at least as important in shaping the dynamics of this type of harmful algal bloom. A deeper understanding of microbiome succession patterns during these rare but destructive events will help guide future efforts to forecast deviations from the natural bloom cycles of the northern Norwegian coastal marine ecosystems that are home to intensive aquaculture activities
Diversity and Selection of Surface Marine Microbiomes in the Atlantic-Influenced Arctic
Serglycin is a proteoglycan highly expressed by immune cells, in which its functions are
linked to storage, secretion, transport, and protection of chemokines, proteases,
histamine, growth factors, and other bioactive molecules. In recent years, it has been
demonstrated that serglycin is also expressed by several other cell types, such as
endothelial cells, muscle cells, and multiple types of cancer cells. Here, we show that
serglycin expression is upregulated in transforming growth factor beta (TGF-b) induced
epithelial-mesenchymal transition (EMT). Functional studies provide evidence that
serglycin plays an important role in the regulation of the transition between the epithelial
and mesenchymal phenotypes, and it is a significant EMT marker gene. We further find
that serglycin is more expressed by breast cancer cell lines with a mesenchymal
phenotype as well as the basal-like subtype of breast cancers. By examining immune
staining and single cell sequencing data of breast cancer tissue, we show that serglycin is
highly expressed by infiltrating immune cells in breast tumor tissue