Sequence-independent characterization of viruses based on the pattern of viral small RNAs produced by the host. [Corrigendum]

published erratum2016 Apr 202016 01 21importedErratum for : Sequence-independent characterization of viruses based on the pattern of viral small RNAs produced by the host. [Nucleic Acids Res. 2015

AI is a viable alternative to high throughput screening: a 318-target study

: High throughput screening (HTS) is routinely used to identify bioactive small molecules. This requires physical compounds, which limits coverage of accessible chemical space. Computational approaches combined with vast on-demand chemical libraries can access far greater chemical space, provided that the predictive accuracy is sufficient to identify useful molecules. Through the largest and most diverse virtual HTS campaign reported to date, comprising 318 individual projects, we demonstrate that our AtomNet® convolutional neural network successfully finds novel hits across every major therapeutic area and protein class. We address historical limitations of computational screening by demonstrating success for target proteins without known binders, high-quality X-ray crystal structures, or manual cherry-picking of compounds. We show that the molecules selected by the AtomNet® model are novel drug-like scaffolds rather than minor modifications to known bioactive compounds. Our empirical results suggest that computational methods can substantially replace HTS as the first step of small-molecule drug discovery

Evaluation of the Effectiveness of Mass Trapping With BG-Sentinel Traps for Dengue Vector Control: A Cluster Randomized Controlled Trial in Manaus, Brazil

We are very grateful to all inhabitants of Cidade Novaneighborhood, who participated in the study. We thank allcontributors from FVS, namely, Luzia Musta ́fa, Ricardo Pas-sos, and Wanderson Sampaio, and with special thanks to allÞeld workers. Contributors from the Virology Department ofthe FMTÐHVD under supervision of Maria Paula GomesMoura ̃o, are highly appreciated for the collection of bloodsamples. We thank the Entomology Department of the FMTÐHVD, which is under supervision of Maria das Grac ̧as ValeBarbosa and Nelson Ferreira Fe ́, for assistance in processingthe catch bags that returned from the Þeld. We thank WouterKarsten Vahl for valuable suggestions on the manuscript.Submitted by Ana Lucia Teixeira ([email protected]) on 2019-09-12T13:50:07Z No. of bitstreams: 1 Evaluation of the Effectiveness of Mass Trapping With BG-SentinelTraps for Dengue Vector Control- A Cluster Randomized ControlledTrial in Manaus, Brazil.pdf: 876241 bytes, checksum: 24b418095d47c83e43d1e702c089d43d (MD5)Approved for entry into archive by Ana Lucia Teixeira ([email protected]) on 2019-09-12T17:10:36Z (GMT) No. of bitstreams: 1 Evaluation of the Effectiveness of Mass Trapping With BG-SentinelTraps for Dengue Vector Control- A Cluster Randomized ControlledTrial in Manaus, Brazil.pdf: 876241 bytes, checksum: 24b418095d47c83e43d1e702c089d43d (MD5)Made available in DSpace on 2019-09-12T17:10:36Z (GMT). No. of bitstreams: 1 Evaluation of the Effectiveness of Mass Trapping With BG-SentinelTraps for Dengue Vector Control- A Cluster Randomized ControlledTrial in Manaus, Brazil.pdf: 876241 bytes, checksum: 24b418095d47c83e43d1e702c089d43d (MD5) Previous issue date: 2014-03The World Bank, theUniversity of the State of Amazonas, CAPES, CNPq (PRONEX-Dengue, grant 550131/2008-8) and INCT-Dengue.Universidade Federal de Minas Gerais. Departamento de Parasitologia. Laboratório de Ecologia Química de Vetores. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Parasitologia. Laboratório de Ecologia Química de Vetores. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Parasitologia. Laboratório de Ecologia Química de Vetores. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Parasitologia. Laboratório de Ecologia Química de Vetores. Belo Horizonte, MG, Brasil.Universidade Nilton Lins. Laboratório de Entomologia Aplicada. Manaus, AM, Brasil.Abteilung Wissenschaft und Diagnostik, Forel Klinik, Switzerland.Fundação Oswaldo Cruz. Presidência. Programa de Computação Científica. Rio de Janeiro, RJ, Brasil.Universidade Federal de Minas Gerais. Laboratório de Vírus. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Laboratório de Vírus. Belo Horizonte, MG, Brasil.Universidade do Estado do Amazonas. Manaus, AM, Brasil.Biogents AG. Weissenburgstr, Regensburg, Germany.The objective of this study was to assess the effectiveness of BG-Sentinel (BGS) traps for mass trapping at the household level to control the dengue vector, Aedes aegypti (L.), in Manaus (Brazil) by performing a cluster randomized controlled trial. After an initial questionnaire and baseline monitoring, 6 out of 12 clusters were randomly allocated to the intervention arm, where participating premises received one BGS trap for mass trapping. The other six clusters did not receive traps and were considered as the control arm. Biweekly monitoring with BGS in both arms assessed the impact of mass trapping. At the end of the study, a serological survey was conducted and a second questionnaire was conducted in the intervention arm. Entomological monitoring indicated that mass trapping with BGS traps significantly reduced the abundance of adult female Ae. aegypti during the first five rainy months. In the subsequent dry season when the mosquito population was lower, no effect of mass trapping was observed. Fewer Ae. aegypti females were measured in the intervention arm during the next rainy period, but no significant difference between arms was observed. The serological survey revealed that in participating houses of mass trapping areas recent dengue infections were less common than in control areas, although this effect was not statistically significant. The majority of participants responded positively to questions concerning user satisfaction. Our results suggest that BGS traps are a promising tool which might be deployed as part of dengue control programs; however, further investigations and larger scale studies are necessary