COMPARISON OF ELISA AND CHEMILUMINESCENCE IMMUNOASSAY METHODS FOR QUANTIFICATION OF HUMAN PLACENTAL GROWTH FACTOR IN SERUM

Placental growth factor (PlGF) is crucial during placental development in early pregnancy. Several studies in pregnancies with complications such as preeclampsia or small for gestational age neonates find that PlGF levels are significantly lower in the first trimester, which implies that the concentration of PlGF could be used as an early screening biomarker for these conditions. This study aimed to compare the performance of chemiluminescence immunoassay (CLIA) and enzyme-linked immunosorbent assay (ELISA) for the quantification of human PlGF in serum. This is a comparative study on 88 pregnant women in the first trimester subjected to measurement of PlGF in serum using two commercially available kits: Human PlGF Quantikine HS ELISA (R&D Systems) and PlGF CLIA (Snibe). The overall coefficient of correlation between the tests was 0.93. When the cut-off value of 40 pg/mL was applied, it dropped significantly to 0.50 towards the lower values, while remaining an excellent 0.91 in the group with higher concentrations of PlGF. While R&D Systems’s ELISA seems to have better sensitivity, it is not very convenient to use for a small number of samples. Snibe’s CLIA automated method is user-friendly, fast and powerful. Both tests show excellent performance when indicating risk-free pregnancies

The corelation between feto-placental unit, fetal biometry and amniotic fluid in pregnant women with preeclampsia in second trimester

Preeclampsia is a pregnancy complication, characterized by high blood pressure, that exceeds 140/90mmHg, documented in two occasions, at least four hours appart and proteinuria 0.3 g or more proteins in urine for 24 hours. It usually begins after 20 gestational weeks, in normotensive women. The incidence is approximately 3-8% of all pregnant women, worldwide. Etiology of preeclampsia is not known yet, but there are theories that explain it: immunologic, genetic, placental ischemia, etc., and all of them contribute to abnormality of spiral arteries, placental site trophoblastic cell dysfunction, inadequate trophoblastic invasion, that finally results with placental hypoperfusion and ischemia. The study is prospective, proceeding at the Department of Pathologic and High Risk Pregnancy, SHGO "Mother Theresa" in Skopje. The data are estimated from March-December 2019. It was done measurment of doppler values of a. uterine dex. et sin., between 20-24 g.a., fetal biometry measurments and the index of amniotic fluid. From all 150 pregnant women included in the study, in 20 of them was found high doppler values and in four of the same group, presence of notch. In 16 pregnant women with high doppler values, it was found intrauterine growth retardation, while in three of them decreased levels of amniotic fluid. All 20 women were followed up by regulary measuring arterial tension and hospitalisation at the beginning of third trimester for doing analizes of presence of proteins in the urine and given adequate therapy. From 20 pregnant women that had high doppler values, hypertension and other changes in ultrasound parameters, in 11 of them was found preeclampsia. Also, it was found positive correlation between high doppler values of a. uterine and notch in second trimester, and appearance of preeclampsia, later in pregnancy and also a correlation between the values of a. uterine, notch, fetal biometry and index of amniotic fluid

The corelation between feto-placental unit, fetal biometry and amniotic fluid in pregnant women with preeclampsia in second trimester

Preeclampsia is a pregnancy complication, characterized by high blood pressure, that exceeds 140/90mmHg, documented in two occasions, at least four hours appart and proteinuria 0.3 g or more proteins in urine for 24 hours. It usually begins after 20 gestational weeks, in normotensive women. The incidence is approximately 3-8% of all pregnant women, worldwide. Etiology of preeclampsia is not known yet, but there are theories that explain it: immunologic, genetic, placental ischemia, etc., and all of them contribute to abnormality of spiral arteries, placental site trophoblastic cell dysfunction, inadequate trophoblastic invasion, that finally results with placental hypoperfusion and ischemia. The study is prospective, proceeding at the Department of Pathologic and High Risk Pregnancy, SHGO "Mother Theresa" in Skopje. The data are estimated from March-December 2019. It was done measurment of doppler values of a. uterine dex. et sin., between 20-24 g.a., fetal biometry measurments and the index of amniotic fluid. From all 150 pregnant women included in the study, in 20 of them was found high doppler values and in four of the same group, presence of notch. In 16 pregnant women with high doppler values, it was found intrauterine growth retardation, while in three of them decreased levels of amniotic fluid. All 20 women were followed up by regulary measuring arterial tension and hospitalisation at the beginning of third trimester for doing analizes of presence of proteins in the urine and given adequate therapy. From 20 pregnant women that had high doppler values, hypertension and other changes in ultrasound parameters, in 11 of them was found preeclampsia. Also, it was found positive correlation between high doppler values of a. uterine and notch in second trimester, and appearance of preeclampsia, later in pregnancy and also a correlation between the values of a. uterine, notch, fetal biometry and index of amniotic fluid