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Antimetastatic potential of anthocyanins from Cordyline australis (G. Forst.) Endl. Red star variety on MCF onco cell lines

Breast cancer is the second most deadly diagnosed lifestyle disease among women. Surgery and chemotherapy are the current treatments of choice; nevertheless, toxicity connected with this underscores the urgency of the demand for the human-friendly drug. 50% of current synthetic drugs available commercially today are either direct or indirect descendants extracted from herbs. Anthocyanins possess many pharmacological activities, including anticancer potential. However, no study on anticancer activity of anthocyanins from Cordyline australis has been reported. Anthocyanins were extracted from fresh leaves using ethanol as solvent. The total anthocyanin was quantified and fractionated by Ultra Performance Liquid Chromatography. Cytotoxicitic effect was carried on diverse cancer cell lines like MCF 7, HCT-116, Caco-2 and SW480 using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay. Based on MTT data, MCF 7 cells were further analyzed by LDH assay, Glutathione-S- transferase (GST), Quercetin reductase, Cytochrome P450 and Caspase 3, 8 and 9 activities. The obtained results were analyzed using ANOVA with a level of significance. Results obtained from MTT assay revealed that the anthocyanin extract carried significant toxic (p < 0.05) specificity against MCF 7 cells (65 ± 2.1 toxicity at 50 µg/ml) when compared to the other onco cells. Remarkable LDH leakage (50.2% vs 50 µg/ml), GST (3.0±0.002 U/mg protein), QR (4.4±0.054 U/mg protein), Cyt P450 activities (0.291±0.01 U/mg protein) were noticed. Caspase 3 (157%), 8 (142%) and 9 (147%) displayed profound activities. These in vitro findings of specific anticancer effects noticed on C. australis anthocyanin extract require further evaluation using animal models. Finally, the obtained findings open up the possibility of developing a lead antimetastatic anthocyanin candidate against deadly breast cancer

Audio Signal Processing Using Time-Frequency Approaches: Coding, Classification, Fingerprinting, and Watermarking

Audio signals are information rich nonstationary signals that play an important role in our day-to-day communication, perception of environment, and entertainment. Due to its non-stationary nature, time- or frequency-only approaches are inadequate in analyzing these signals. A joint time-frequency (TF) approach would be a better choice to efficiently process these signals. In this digital era, compression, intelligent indexing for content-based retrieval, classification, and protection of digital audio content are few of the areas that encapsulate a majority of the audio signal processing applications. In this paper, we present a comprehensive array of TF methodologies that successfully address applications in all of the above mentioned areas. A TF-based audio coding scheme with novel psychoacoustics model, music classification, audio classification of environmental sounds, audio fingerprinting, and audio watermarking will be presented to demonstrate the advantages of using time-frequency approaches in analyzing and extracting information from audio signals.</p

Solvent Extraction Behaviour of Tris(oxalato)Co(III) with Long-chain Amines: Part II - Extraction from Hydrochloric & Sulphuric Acid Solutions

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On the diffraction of light by spherical obstacles

The diffraction of light inside the shadow, thrown by a small source of light, of a sphere and a circular disc of the same diameter, was studied, with special reference to the relative intensities of the central bright spots. With the source at about 2 metres from the obstacles, with a quarter-inch polished steel ball, the bright spot could be detected visually up to 3 cm. behind the obstacle, while with a steel disc of the same diameter, with the edges perfectly sharp, smooth and circular, the spot could be traced up to 2 cm. The relative intensities of the two spots were studied at different distances behind the obstacles, qualitatively by photography and quantitatively by visual photometry. At small distances behind the obstacles, the spot inside the shadow of the sphere is much feebler than the disc-spot, however approximating to the latter as we reach farther back from the obstacles, but even at 100 cm. remaining appreciably feebler. A general explanation is suggested

Polynuclear aromatic hydrocarbon carcinogens as antiestrogens in MCF-7 human breast cancer cells: role of the Ah receptor

Treatment of MCF-7 cells with 1.0 &#956;M 3-methylcholanthrene (MC) caused a decrease in cell proliferation and [3H]thymidine uptake whereas no effects were observed at a lower (0.1 &#956;M) concentration. Co-treatment of the cells with 1 nM 17&#946;-estradiol plus 0.1 or 1.0 &#956; MC resulted in a significant inhibition of 17&#946;-estradiol-induced growth and [3H]thymidine uptake. MC also inhibited the 17&#946;-estradiol-induced secretion of the 52 kDa protein (procathepsin D) in MCF-7 cells and caused a concentration-dependent decrease in the nuclear estrogen receptor (ER) as determined by either velocity sedimentation analysis or immunoquantitation with human ER antibodies. The effects of several different polynuclear aromatic hydrocarbon (PAH) congeners on the nuclear ER in MCF-7 cells were also determined. Only those congeners which bound to the aryl hydrocarbon (Ah) receptor, namely benzo[a]pyrene, benz[a]anthracene, 7,12-dimethylbenz[a]anthracene and MC, caused a decrease in nuclear ER levels. In contrast, benzo[ghi]perylene, a congener which did not bind to the Ah receptor did not affect nuclear ER levels in MCF-7 cells. Moreover, with some congeners the decrease in nuclear ER levels could be observed without any significant induction of ethoxyresorufin O-deethylase activity, a P4501A1-dependent monooxygenase. These data suggest that the Ah receptor liganded with MC and related PAHs induced a broad spectrum of antiestrogenic responses in MCF-7 cells and complements the results of previous studies which report the antiestrogenic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin and other halogenated aromatics which are also Ah receptor agonists