Development of an in vitro Dual Culture System for Grapevine and Xiphinema index as a Tool for Virus Transmission

Grapevine fanleaf virus (GFLV) is a nepovirus that is transmitted to grapevines by the ectoparasitic nematodeXiphinema index. GFLV causes severe losses in yield and quality in viticulture worldwide. Presently, laborious andtime-consuming field trials or greenhouse tests are necessary for screening putative GFLV resistance in new grapegenotypes developed in breeding programmes. We developed an in vitro dual culture system for grapevines andnematode vectors that requires less time and space than inoculation experiments done in the greenhouse. Virusinfection of in vitro grapevines was investigated using immunocapture-reverse transcriptase-polymerase chainreaction (IC-RT-PCR) analysis. The development of root galls induced by feeding nematodes on in vitro grapevineswas also analysed. Virus infection in grapevines in the dual culture with viruliferous nematodes was detected sixweeks post-inoculation. Root galls were always absent from parasitised in vitro grapevines with detectable virusinfection, whereas they developed on some parasitised, but virus-negative tested grapevines. Therefore, root gallscannot be used as a reliable indicator for parasitism and virus transmission

Analysis of the acquisition and multiplication efficiency of different strains of Ca. Phytoplasma mali by the vector Cacopsylla picta

Based on previous observations during long-term acquisition and transmission trials, studies were carried out under standardized conditions in order to analyse the acquisition and multiplication efficiencies of different strains of Candidatus Phytoplasma mali by different developmental stages of Cacopsylla picta. The acquisition of Ca. P. mali from micropropagated plants infected with different strains was tested for nymphs, larval stages and new adults of C. picta. When born on infected plants a nearly 100% acquisition was achieved for all strains of Ca. P. mali by C. picta. Differences in acquisition efficiency were observed for new generation adults which acquired the phytoplasma as imagines. The multiplication efficiency of the different Ca. P. mali strains inside the insects was analysed by quantitative real-time PCR. Significant differences in the capacity of the different strains to colonise the insect were found. Despite high acquisition rates only few subsequent transmission events to healthy test plants could be recorded

Influence of Apple stem grooving virus on Malus sieboldii-derived apple proliferation resistant rootstocks

Apple stem grooving virus (ASGV, Capillovirus) is widely spread in apple growing regions. As it causes no symptoms on most cultivated apple varieties and rootstocks it is considered latent in Malus x domestica. In Asia, however, ASGV has been found associated with topworking disease of apple rootstocks originating from Malus sieboldii. Recently, M. sieboldii and its hybrids have gained new interest in Europe as they confer resistance to apple proliferation (AP) disease. A new breeding program aiming to develop AP-resistant rootstocks of agronomic value for modern apple culture, reported unexpected tree decline which was to be associated with ASGV. As little information is available on the variability of ASGV isolates in Germany, the complete genome of a German isolate of ASGV associated with tree decline was cloned and sequenced. Sequence comparisons with available ASGV isolates revealed two regions of high variability in the genome. The genetic variability of additional isolates from Germany and other countries were collected and the variable areas characterised. In addition ASGV was successfully maintained in micropropagated apple trees and could be transmitted by in vitro grafting to various genotypes, making it possible to study in vitro the effect of the virus and virus/phytoplama combination on M. sieboldii-derived genotypes. Keywords: Latent apple viruses, Candidatus Phytoplasma mali, micropropagation, in vitro grafting, genetic variabilit

Trust-building in temporary public health partnerships: a qualitative study of the partnership formation process of a Covid-19 test, trace and protect service

Background: Public health initiatives require coordinated efforts from healthcare, social services and other service providers. Organisational theory tells us that trust is essential for reaching collaborative effectiveness. This paper explores the drivers for initiating and sustaining trust in a temporary public health partnership, in response to a sudden health threat. Methods: This qualitative study analysed the formation process of a multisector partnership for a Covid-19 contact tracing service. Data was collected through 12 interviews, two focus groups, one feedback workshop, and an online survey with workforce members from all seven partner organisations. Purposive maximum variation sampling was used to capture the reflections and experiences of workforce members from all seven partner organisations. A deductive code scheme was used to identify drivers for building and sustaining trust in inter-organisational collaboration. Results: Relational mechanisms emanating from the commitment to the common aim, shared norms and values, and partnership structures affected trust-building. Shared values and the commitment to the common aim appeared to channel partners’ behaviour when interacting, resulting in being perceived as a fair, reliable and supportive partner. Shared values were congruent with the design of the partnership in terms of governance structure and communication lines reflecting flat hierarchies and shared decision-making power. Tensions between partner organisations arose when shared values were infringed. Conclusions: When managing trust in a collaboration, partners should consider structural components like governance structure, organisational hierarchy, and communication channels to ensure equal power distribution. Job rotation, recruitment of candidates with the desired personality traits and attitudes, as well as training and development, encourage inter-organisational networking among employees, which is essential for building and strengthening relationships with partner organisations. Partners should also be aware of managing relational dynamics, channelling behaviours through shared values, objectives and priorities and fostering mutual support and equality among partner organisations

Genomeditierung von Pflanzen im internationalen und rechtlichen Kontext

Das Urteil des Europäischen Gerichtshofs (EuGH) vom Juli 2018 und die damit verbundenen Auflagen für die Zulassung Genom-editierter Pflanzen machen die Nutzung entsprechender Verfahren für die Pflanzenzüchtung in Europa nahezu unmöglich. Unser Mitglied, die Gesellschaft für Pflanzenbiotechnologie, begleitet diese Entwicklungen mit großer Sorge. Hier stellt sie sich und ihre Arbeit vor

Still looking in the wrong place: literature-based evidence of why patients really attend an emergency department

Presenting complaints at an Emergency Department (ED) that could (and should) have been seen in primary care are discussed in the literature as ‘inappropriate use’ of hospital-based emergency services. These medically inappropriate requests are perceived as a threat to service quality, implying more costs than necessary. Using Systems Thinking/Dynamics, this paper introduces an evidence-based framework to explain why people increasingly attend an ED instead of a primary-care-based emergency facility, with patient demographics (age and deprivation), signposting sources and patients' perceptions (reflecting latent needs) identified as the main determinates of ED use. The framework makes explicit the endogenous dynamics of referral, service choice and service reputation (where expectations and confirming experiences are recursively shaped over time). The work can be employed at the strategic level as a framework to inform attendance management when evaluating or altering the healthcare system. This is achieved by presenting how the healthcare system responds to patient encounters and how patient behaviour adapts in response. At the operational level, the presented framework enables modellers and healthcare planners to develop hospital-based and primary-care-based emergency care interventions with empathy and compassion for patients. We highlight opportunities for future work as the healthcare system is complex and requires more in-depth exploration/modelling to complete the picture

Presence of the Aphid, Chaetosiphon fragaefolii, on Strawberry in Argentina

Seasonal abundance of the strawberry aphid complex under different agronomic practices in the outskirts of La Plata, Argentina was studied on strawberry, Fragaria x ananassa Duchesne (Rosales: Rosaceae). Aphid densities were low in strawberry fields in which insecticides and fungicides were used. In addition to Aphis gossypii, Aphis fabae, Mysus persicae and Macrosiphum euphorbiae, the aphid, Chaetosiphon fragaefolii (Cockerell) (Homoptera: Aphididae), was recorded for the first time in this horticultural area. Life history and some demographic parameters were calculated for C. fragaefolii. The mean duration of nymphal stages was 10.44 days, the oviposition period was 11.8 days, and the mean number of nymph/female/day was 2.4 ± 0.3. Demographic parameters analyzed included the net reproductive rate Ro = 14.55 ± 0.096 nymph/female, generation time T=16.91 ± 0.035 days, and the intrinsic rate of increase rm = 0.158 ± (0.004). No parasites were found associated with C. fragaefolli. The pathogenic fungus, Entomophthora planchoniana Cornu (Zygomycetes: Entomophthorales) was the main mortality factor. Although aphids are not the main pests in strawberry fields, C. fragaefolii can be a serious problem because it can transmit several virus diseases of strawberry. Greater knowledge of life history traits and mortality factors of this species is needed in order to design appropriate control strategies

An Update on the Intracellular and Intercellular Trafficking of Carmoviruses

[EN] Despite harboring the smallest genomes among plant RNA viruses, carmoviruses have emerged as an ideal model system for studying essential steps of the viral cycle including intracellular and intercellular trafficking. Two small movement proteins, formerly known as double gene block proteins (DGBp1 and DGBp2), have been involved in the movement throughout the plant of some members of carmovirus genera. DGBp1 RNA-binding capability was indispensable for cell-to-cell movement indicating that viral genomes must interact with DGBp1 to be transported. Further investigation on Melon necrotic spot virus (MNSV) DGBp1 subcellular localization and dynamics also supported this idea as this protein showed an actin-dependent movement along microfilaments and accumulated at the cellular periphery. Regarding DGBp2, subcellular localization studies showed that MNSV and Pelargonium flower break virus DGBp2s were inserted into the endoplasmic reticulum (ER) membrane but only MNSV DGBp2 trafficked to plasmodesmata (PD) via the Golgi apparatus through a COPII-dependent pathway. DGBp2 function is still unknown but its localization at PD was a requisite for an efficient cell-to-cell movement. It is also known that MNSV infection can induce a dramatic reorganization of mitochondria resulting in anomalous organelles containing viral RNAs. These putative viral factories were frequently found associated with the ER near the PD leading to the possibility that MNSV movement and replication could be spatially linked. Here, we update the current knowledge of the plant endomembrane system involvement in carmovirus intra-and intercellular movement and the tentative model proposed for MNSV transport within plant cells.This work was funded by grant BIO2014-54862-R from the Spanish Direccion General de Investigacion Cientifica y Tecnica (DGICYT) and the Prometeo Program GV2014/010 from the Generalitat Valenciana.Navarro Bohigues, JA.; Pallás Benet, V. (2017). An Update on the Intracellular and Intercellular Trafficking of Carmoviruses. Frontiers in Plant Science. 8:1-7. https://doi.org/10.3389/fpls.2017.01801S178Adams, M. J., Lefkowitz, E. J., King, A. M. Q., Harrach, B., Harrison, R. L., Knowles, N. J., … Davison, A. J. (2016). Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2016). Archives of Virology, 161(10), 2921-2949. doi:10.1007/s00705-016-2977-6Blake, J. A., Lee, K. W., Morris, T. J., & Elthon, T. E. (2007). 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