In search of an appropriate abstraction level for motif annotations

In: Proceedings of the 2012 Workshop on Computational Models of Narrative, (pp. 22-28).

Molecular characterization of the CmbR activator-binding site in the metC-cysK promoter region in Lactococcus lactis

The metC-cysK operon involved in sulphur metabolism in Lactococcus lactis is positively regulated by the LysR-type protein CmbR. Transcription from the metC promoter is activated when concentrations of methionine and cysteine in the growth medium are low. The metC promoter region contains two direct and three inverted repeats. Deletion analysis indicated that direct repeat 2 (DR2) is required for activation of the metC promoter by CmbR. Gel mobility shift assays confirmed that CmbR binds to a 407 bp DNA fragment containing the rnetC promoter. This binding was stimulated by O-acetyl-L-serine. Competition experiments with deletion variants of the metC promoter showed that CmbR binding only occurred with fragments containing an intact DR2, confirming that DR2 is the CmbR binding site within the metC promoter

Application of Quantitative MRI Techniques in Ischemic and Congenital Heart Disease image-guided therapy

It is estimated that each year, 30,000 people in the Netherlands suffer from myocardial infarction, and is in fact a major public health care burden. Next to ischaemic heart disease (IHD), thanks to modern medicine, more and more patients with congenital heart disease (CHD) reach adulthood, but nonetheless require lifelong professional care. Imaging techniques are essential in these two groups of patients for establishing diagnosis, guiding therapy and predicting outcomes. This thesis investigated the application of cardiac MRI in patients with IHD and CHD and evaluated novel non-invasive MRI techniques in both humans and in a porcine model. In this thesis we showed that cardiac MRI is an excellent tool for the evaluation of atherosclerotic and congenital cardiovascular disease. It is an excellent tool in predicting outcome after STEM! in patients undergoing primary percutaneous coronary intervention. Furthermore, studied in this thesis, the incidence of per procedural complications in patients with CHD seems higher than in the general population suggesting that placement of a pacemaker device might be challenging in this group of patients

Reversed lateral circulation in a sharp estuarine bend with weak stratification

Author Posting. © American Meteorological Society, 2019. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 49(6), (2019):1619-1637, doi:10.1175/JPO-D-18-0175.1.Although the hydrodynamics of river meanders are well studied, the influence of curvature on flow in estuaries, with alternating tidal flow and varying water levels and salinity gradients, is less well understood. This paper describes a field study on curvature effects in a narrow salt-marsh creek with sharp bends. The key observations, obtained during times of negligible stratification, are 1) distinct differences between secondary flow during ebb and flood, with helical circulation as in rivers during ebb and a reversed circulation during flood, and 2) maximum (ebb and flood) streamwise velocities near the inside of the bend, unlike typical river bend flow. The streamwise velocity structure is explained by the lack of a distinct point bar and the relatively deep cross section in the estuary, which means that curvature-induced inward momentum redistribution is not overcome by outward redistribution by frictional and topographic effects. Through differential advection of the along-estuary salinity gradient, the laterally sheared streamwise velocity generates lateral salinity differences, with the saltiest water near the inside during flood. The resulting lateral baroclinic pressure gradient force enhances the standard helical circulation during ebb but counteracts it during flood. This first leads to a reversed secondary circulation during flood in the outer part of the cross section, which triggers a positive feedback mechanism by bringing slower-moving water from the outside inward along the surface. This leads to a reversal of the vertical shear in the streamwise flow, and therefore in the centrifugal force, which further enhances the reversed secondary circulation.This project was funded by NSF Grant OCE-1634490. During this work W.M. Kranenburg was supported as USGS Postdoctoral Scholar at Woods Hole Oceanographic Institution. A.M.P. Garcia was supported by the Michael J. Kowalski Fellowship in Ocean Science and Engineering (AMPG), and the Diversity Fellowship of the MIT Office of the Dean of Graduate Education (AMPG). The authors thank Jay Sisson for the technical support and Peter Traykovski for providing the bathymetric data. Also, the suggestions for improvement by Dr. K. Blanckaert and an anonymous reviewer are thankfully acknowledged

Thoracic and Lumbar Vertebral Bone Mineral Density Changes in a Natural Occurring Dog Model of Diffuse Idiopathic Skeletal Hyperostosis

Ankylosing spinal disorders can be associated with alterations in vertebral bone mineral density (BMD). There is however controversy about vertebral BMD in patients wuse idiopathic skeletal hyperostosis (DISH). DISH in Boxer dogs has been considered a natural occurring disease model for DISH in people. The purpose of this study was to compare vertebral BMD between Boxers with and without DISH. Fifty-nine Boxers with (n=30) or without (n=29) DISH that underwent computed tomography were included. Vertebral BMD was calculated for each thoracic and lumbar vertebra by using an earlier reported and validated protocol. For each vertebral body, a region of interest was drawn on the axial computed tomographic images at three separate locations: immediately inferior to the superior end plate, in the middle of the vertebral body, and superior to the inferior end plate. Values from the three axial slices were averaged to give a mean Hounsfield Unit value for each vertebral body. Univariate statistical analysis was performed to identify factors to be included in a multivariate model. The multivariate model including all dogs demonstrated that vertebral DISH status (Coefficient 24.63; 95% CI 16.07 to 33.19; p <0.001), lumbar vertebrae (Coefficient -17.25; 95% CI -23.42 to -11.09; p < 0.01), and to a lesser extent higher age (Coefficient -0.56; 95% CI -1.07 to -0.05; p = 0.03) were significant predictors for vertebral BMD. When the multivariate model was repeated using only dogs with DISH, vertebral DISH status (Coefficient 20.67; 95% CI, 10.98 to 30.37; p < 0.001) and lumbar anatomical region (Coefficient -38.24; 95% CI, -47.75 to -28.73; p < 0.001) were again predictors for vertebral BMD but age was not. The results of this study indicate that DISH can be associated with decreased vertebral BMD. Further studies are necessary to evaluate the clinical importance and pathophysiology of this finding