Solar microflares: a case study on temperatures and the Fe XVIII emission

In this paper, we discuss the temperature distribution and evolution of a microflare, simultaneously observed by Hinode XRT, EIS, and SDO AIA. We find using EIS lines that during peak emission the distribution is nearly isothermal and peaked around 4.5 MK. This temperature is in good agreement with that obtained from the XRT filter ratio, validating the use of XRT to study these small events, invisible by full-Sun X-ray monitors such as GOES. The increase in the estimated Fe XVIII emission in the AIA 94 {\AA} band can mostly be explained with the small temperature increase from the background temperatures. The presence of Fe XVIII emission does not guarantee that temperatures of 7 MK are reached, as is often assumed. We also revisit with new atomic data the temperatures measured by a SoHO SUMER observation of an active region which produced microflares, also finding low temperatures (3 - 4 MK) from an Fe XVIII / Ca XIV ratio.Comment: 12-13 pages, 17 figures (22 eps-files), 4 tables, accepted by Astronomy and Astrophysic

Identification and functional expression of the plasma membrane calcium ATPase gene family from Caenorhabditis elegans.

Calcium-pumping ATPases are an essential component of the intracellular calcium homeostasis system and have been characterized in a large variety of species and cell types. In mammalian genomes, these proteins are encoded by gene families whose individual members feature complex tissue-specific expression and alternative splicing. In the search for a less complex system that is more amenable to genetic manipulation, we have identified a family of three genes (mca-1, mca-2, and mca-3) encoding putative calcium ATPases in the Caenorhabditis elegans Genome Project data and completed their transcript structure. In this work, we report the cloning and functional expression of the mca-1 gene, which encodes a calcium-stimulated ATPase whose features resemble those of the plasma membrane calcium adenosine triphosphatase family of mammalian cells and appears to be regulated by a multipartite promoter

Flares on active M-type stars observed with XMM-Newton and Chandra.

M-type red dwarfs are among the most active stars. Their light curves display random variability of rapid increase and gradual decrease in emission. It is believed that these large energy events, or flares, are the manifestation of the permanently reforming magnetic field of the stellar atmosphere. Stellar coronal flares are observed in the radio, optical, ultraviolet and X-rays. With the new generation of X-ray telescopes, XMM-Newton and Chandra, it has become possible to study these flares in much greater detail than ever before. This thesis focuses on three core issues about flares: (i) how their X-ray emission is correlated with the ultraviolet, (ii) using an oscillation to determine the loop length and the magnetic field strength of a particular flare, and (iii) investigating the change of density sensitive lines during flares using high-resolution X-ray spectra, (i) It is known that flare emission in different wavebands often correlate in time. However, here is the first time where data is presented which shows a correlation between emission from two different wavebands (soft X-rays and ultraviolet) over various sized flares and from five stars, which supports that the flare process is governed by common physical parameters scaling over a large range. (ii) As it is impossible to spatially resolve any but a very few giant stars, the only information on spatial dimensions as well as the magnetic field strength of stellar coronae has to come from indirect measurements. Using wavelet analysis, I isolated the first stellar X-ray flare oscillation. Interpreting it as a standing coronal flare loop oscillation, I derived a flare loop length as well as the magnetic field strength for this X-ray flare. (iii) The high-resolution soft X-ray spectra of Chandra and XMM-Newton allow us to determine temperatures, densities and abundances of the stellar coronae. De spite a low signal-to-noise ratio because of the relatively short duration of a flare, we find that, if adding up the photons of several flares, certain density sensitive spectral lines change significantly between quiescent and flaring states. This project led on to investigate the flaring spectrum further, and it is found that the plasma is no longer in collisional ionisation equilibrium, but that it is dominated by recombinations

Protein Deimination and Extracellular Vesicle Profiles in Antarctic Seabirds

Pelagic seabirds are amongst the most threatened of all avian groups. They face a range of immunological challenges which seem destined to increase due to environmental changes in their breeding and foraging habitats, affecting prey resources and exposure to pollution and pathogens. Therefore, the identification of biomarkers for the assessment of their health status is of considerable importance. Peptidylarginine deiminases (PADs) post-translationally convert arginine into citrulline in target proteins in an irreversible manner. PAD-mediated deimination can cause structural and functional changes in target proteins, allowing for protein moonlighting in physiological and pathophysiological processes. PADs furthermore contribute to the release of extracellular vesicles (EVs), which play important roles in cellular communication. In the present study, post-translationally deiminated protein and EV profiles of plasma were assessed in eight seabird species from the Antarctic, representing two avian orders: Procellariiformes (albatrosses and petrels) and Charadriiformes (waders, auks, gulls and skuas). We report some differences between the species assessed, with the narrowest EV profiles of 50−200 nm in the northern giant petrel Macronectes halli, and the highest abundance of larger 250−500 nm EVs in the brown skua Stercorarius antarcticus. The seabird EVs were positive for phylogenetically conserved EV markers and showed characteristic EV morphology. Post-translational deimination was identified in a range of key plasma proteins critical for immune response and metabolic pathways in three of the bird species under study; the wandering albatross Diomedea exulans, south polar skua Stercorarius maccormicki and northern giant petrel. Some differences in Gene Ontology (GO) biological and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways for deiminated proteins were observed between these three species. This indicates that target proteins for deimination may differ, potentially contributing to a range of physiological functions relating to metabolism and immune response, as well as to key defence mechanisms. PAD protein homologues were identified in the seabird plasma by Western blotting via cross-reaction with human PAD antibodies, at an expected 75 kDa size. This is the first study to profile EVs and to identify deiminated proteins as putative novel plasma biomarkers in Antarctic seabirds. These biomarkers may be further refined to become useful indicators of physiological and immunological status in seabirds—many of which are globally threatened

Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination

The bovine immune system is known for its unusual traits relating to immunoglobulin and antiviral responses. Peptidylarginine deiminases (PADs) are phylogenetically conserved enzymes that cause post-translational deimination, contributing to protein moonlighting in health and disease. PADs also regulate extracellular vesicle (EV) release, forming a critical part of cellular communication. As PAD-mediated mechanisms in bovine immunology and physiology remain to be investigated, this study profiled deimination signatures in serum and serum-EVs in Bos taurus. Bos EVs were poly-dispersed in a 70−500 nm size range and showed differences in deiminated protein cargo, compared with whole sera. Key immune, metabolic and gene regulatory proteins were identified to be post-translationally deiminated with some overlapping hits in sera and EVs (e.g., immunoglobulins), while some were unique to either serum or serum-EVs (e.g., histones). Protein−protein interaction network analysis of deiminated proteins revealed KEGG pathways common for serum and serum-EVs, including complement and coagulation cascades, viral infection (enveloped viruses), viral myocarditis, bacterial and parasitic infections, autoimmune disease, immunodeficiency intestinal IgA production, B-cell receptor signalling, natural killer cell mediated cytotoxicity, platelet activation and hematopoiesis, alongside metabolic pathways including ferroptosis, vitamin digestion and absorption, cholesterol metabolism and mineral absorption. KEGG pathways specific to EVs related to HIF-1 signalling, oestrogen signalling and biosynthesis of amino acids. KEGG pathways specific for serum only, related to Epstein−Barr virus infection, transcription mis-regulation in cancer, bladder cancer, Rap1 signalling pathway, calcium signalling pathway and ECM-receptor interaction. This indicates differences in physiological and pathological pathways for deiminated proteins in serum-EVs, compared with serum. Our findings may shed light on pathways underlying a number of pathological and anti-pathogenic (viral, bacterial, parasitic) pathways, with putative translatable value to human pathologies, zoonotic diseases and development of therapies for infections, including anti-viral therapies

Extracellular vesicle signatures and protein citrullination are modified in shore crabs (Carcinus maenas) infected with Hematodinium sp

Epizootiologists recurrently encounter symbionts and pathobionts in the haemolymph (blood equivalent) of shellfish. One such group is the dinoflagellate genus , which contains several species that cause debilitating disease in decapod crustaceans. The shore crab acts as a mobile reservoir of microparasites, including sp., thereby posing a risk to other co-located commercially important species, e.g. velvet crabs ( ). Despite the widespread prevalence and documented seasonality of infection dynamics, there is a knowledge gap regarding host-pathogen antibiosis, namely, how avoids the host's immune defences. Herein, we interrogated the haemolymph of -positive and -negative crabs for extracellular vesicle (EV) profiles (a proxy for cellular communication), alongside proteomic signatures for post-translational citrullination/deimination performed by arginine deiminases, which can infer a pathologic state. Circulating EV numbers in parasitized crab haemolymph were reduced significantly, accompanied by smaller EV modal size profiles (albeit non-significantly) when compared to -negative controls. Differences were observed for citrullinated/deiminated target proteins in the haemolymph between the parasitized and control crabs, with fewer hits identified overall in the former. Three deiminated proteins specific to parasitized crab haemolymph were actin, Down syndrome cell adhesion molecule (DSCAM), and nitric oxide synthase - factors that contribute to innate immunity. We report, for the first time, sp. could interfere with EV biogenesis, and that protein deimination is a putative mechanism of immune-modulation in crustacean- interactions

Complement component C4-like protein in Atlantic cod (<i>Gadus morhua</i> L.) - Detection in ontogeny and identification of post-translational deimination in serum and extracellular vesicles

The complement system is a critical part of teleost immune defences, with complement component C4 forming part of the classical and lectin pathways. Cod C4-like protein was isolated from plasma, specific antibodies generated and C4-like protein was assessed in cod sera, mucus and in extracellular vesicles (EVs) from serum and mucus. Higher levels of C4-like protein were detected in serum- than mucus-derived EVs. Post-translational deimination, caused by conversion of arginine into citrulline, can affect protein structure and function. Here we detected deiminated forms of C4-like protein in cod serum and at lower levels in mucus. C4-like protein was also found in deiminated form at low levels in EVs from both serum and mucus. C4-like protein was assessed by immunohistochemistry in cod larvae and detected in a range of organs including in liver, kidney, gut, muscle, skin and mucus, as well as in neuronal tissues of the brain, spinal cord and eye. This abundance of C4-like protein during early development may indicate roles in tissue remodelling, in addition to immune defences. The presence of deiminated C4-like protein in serum and mucosa, as well as in EVs, may suggest C4 protein moonlighting via post-translational deimination

Long Term Potentiation (LTP) and Long Term Depression (LTD) Cause Differential Spatial Redistribution of the Synaptic Vesicle Protein Synaptophysin in the Middle Molecular Layer of the Dentate Gyrus in Rat Hippocampus

The presynaptic modifications that accompany long-term changes in synaptic plasticity are still not fully understood. Synaptophysin is a major synaptic vesicle protein involved in neurotransmitter release. We have used quantitative electron microscopy to study synaptophysin (Syn) immunolabelling in the hippocampus of adult rats 24h after induction in vivo of long term potentiation (LTP), and long term depression (LTD). Electrodes were implanted chronically in hippocampus with stimulation at either the medial (MPP) or lateral perforant path (LPP). 24h following induction of LTP or LTD rats were rapidly perfusion fixed and hippocampal tissue processed to electron microscopy via freeze substitution method. Anti-synaptophysin post-embedding immunolabelling was performed and tissue was imaged in the middle molecular layer (MML) of the dentate gyrus. There was a significant decrease in number of Syn labelled vesicles per unit area of bouton after LTP, but not LTD. An analysis of the spatial distribution of Syn labelled synaptic vesicles showed an increase in nearest neighbour distances, more so in the LTP than the LTD group, which is consistent with the overall decrease of Syn after LTP. These data are in agreement with the suggestion that Syn is involved in clathrin-dependent and “kiss and run” endocytosis which occurs perisynaptically. Thus, an increase in release of neurotransmitter and in consequence endocytosis would be consistent with an increased active zone distance for vesicles containing Syn