20 research outputs found
Multi-level analysis of the gut-brain axis shows autism spectrum disorder-associated molecular and microbial profiles
Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by heterogeneous cognitive, behavioral and communication impairments. Disruption of the gut-brain axis (GBA) has been implicated in ASD although with limited reproducibility across studies. In this study, we developed a Bayesian differential ranking algorithm to identify ASD-associated molecular and taxa profiles across 10 cross-sectional microbiome datasets and 15 other datasets, including dietary patterns, metabolomics, cytokine profiles and human brain gene expression profiles. We found a functional architecture along the GBA that correlates with heterogeneity of ASD phenotypes, and it is characterized by ASD-associated amino acid, carbohydrate and lipid profiles predominantly encoded by microbial species in the genera Prevotella, Bifidobacterium, Desulfovibrio and Bacteroides and correlates with brain gene expression changes, restrictive dietary patterns and pro-inflammatory cytokine profiles. The functional architecture revealed in age-matched and sex-matched cohorts is not present in sibling-matched cohorts. We also show a strong association between temporal changes in microbiome composition and ASD phenotypes. In summary, we propose a framework to leverage multi-omic datasets from well-defined cohorts and investigate how the GBA influences ASD
CONSISTENCY IN ASSESSING PHYSICAL DEVELOPMENT OF CHILDREN WITH CEREBRAL PALSY ACCORDING TO REGIONAL AND SPECIALIZED CENTILE SCALES: A POPULATION-BASED CROSS-SECTIONAL STUD
Background. Children with infantile cerebral palsy (ICP) often have a physical developmental delay due to protein-energy malnutrition (PEM). The assessment of such abnormalities using different centile scales may lead to inconsistent results.Objective. Our aim was to study the consistency in assessing physical development of children with ICP using regional and specialized centile scales.Methods. The assessment of physical development with determination of the proportion of patients with PEM (weight-age and/or heightage values < 10th percentile) was carried out using regional centile scales and the Life Expectancy Project scales taking into account motor disorder levels (IβV) according to GMFCS. The assessment of motor disorders was performed by neurologists of healthcare organizations in 54 municipalities of the Sverdlovsk Region. The accounting period was from September 2016 to January 2017.Results. According to regional centile scales, PEM was identified in 272 (35.7%) children; according to the Life Expectancy Project scales β in 56 (7.4%) out of 761 children with ICP (McNemar's criterion, p < 0.001). Using regional scales the following results were registered: children with GMFCS I had PEM 5 times less than children with GMFCS V β 10 (12.5%) and 107 (66%) cases, respectively (κ2 criterion, p < 0.001). When assessing physical development of children with specialized scales, PEM occurred in 4 (5%) patients with GMFCS I and 18 (11.1%) with GMFCS V (p < 0.001).Conclusion. Specialized scales for assessing physical development less often indicate the presence of PEM in children with ICP. When using regional and to a much lesser extent specialized centile scales, the detection rate for PEM cases depends on the level of motor disorders
Neurotoxic Complications of Chemotherapy in Children. Literature Review
The article provides the review of literature on the issue of neurotoxic complications of chemotherapy in children. The data search was carried out in the PubMed, CyberLeninka, RSCI databases and others. There are many works devoted to studying the distribution, structure, and clinical features of chemo-induced neurological disorders in modern literature. In general, analysis of literature has shown that the incidence of neurotoxic complications varies widely depending on the research methods and used chemo-ther-apeutic drugs. Manifestations of chemotherapy-induced neurological disorders are quite diverse and accompanied by the central and peripheral nervous system damage. However, it has been shown that the platinum-based drugs have effective ototoxic effect with the damage of the auditory nerve. The Vincristine often causes peripheral polyneuropathy. Methotrexate and Ifosfamide cause metabolic encephalopathy. Cytarabineβs neurotoxicity is the damage of the cerebellum. The addition of neurological symptoms leads to decrease in the quality of life of patients. Some studies present the results of study of the causes and pathogenetic manifestations of neurotoxic reactions, changes in the genome associated with the development of specific toxic effects are identified. Despite the fact that many pathogenetic mechanisms of chemotherapy-induced neurotoxic complications have been revealed, the significance of laboratory predictors for prognosing and early assessment of these complications has not been essentially established yet, thus, there is the need for further study of this topic
Restore Blue Tungsten Oxide with Hydrogen
Studied the mechanism and kinetics of tungsten oxide recovery blue hydrogen and tungsten powder with options for unit surface for production of high quality tungsten wire.ΠΠ·ΡΡΠ΅Π½Ρ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌ ΠΈ ΠΊΠΈΠ½Π΅ΡΠΈΠΊΠ° Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΡ ΡΠΈΠ½Π΅Π³ΠΎ ΠΎΠΊΡΠΈΠ΄Π° Π²ΠΎΠ»ΡΡΡΠ°ΠΌΠ° Π²ΠΎΠ΄ΠΎΡΠΎΠ΄ΠΎΠΌ ΠΈ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΡ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ Π²ΠΎΠ»ΡΡΡΠ°ΠΌΠΎΠ²ΠΎΠ³ΠΎ ΠΏΠΎΡΠΎΡΠΊΠ° Ρ ΡΠ΄Π΅Π»ΡΠ½ΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΡΡ, ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΠ²Π°ΡΡΠ΅ΠΉ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΡΡΠ²ΠΎ Π½Π΅ΠΏΡΠΎΠ²ΠΈΡΠ°ΡΡΠ΅ΠΉ Π²ΠΎΠ»ΡΡΡΠ°ΠΌΠΎΠ²ΠΎΠΉ ΠΏΡΠΎΠ²ΠΎΠ»ΠΎΠΊΠΈ
Restore Blue Tungsten Oxide with Hydrogen
Studied the mechanism and kinetics of tungsten oxide recovery blue hydrogen and tungsten powder with options for unit surface for production of high quality tungsten wire.ΠΠ·ΡΡΠ΅Π½Ρ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌ ΠΈ ΠΊΠΈΠ½Π΅ΡΠΈΠΊΠ° Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΡ ΡΠΈΠ½Π΅Π³ΠΎ ΠΎΠΊΡΠΈΠ΄Π° Π²ΠΎΠ»ΡΡΡΠ°ΠΌΠ° Π²ΠΎΠ΄ΠΎΡΠΎΠ΄ΠΎΠΌ ΠΈ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΡ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ Π²ΠΎΠ»ΡΡΡΠ°ΠΌΠΎΠ²ΠΎΠ³ΠΎ ΠΏΠΎΡΠΎΡΠΊΠ° Ρ ΡΠ΄Π΅Π»ΡΠ½ΠΎΠΉ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΡΡ, ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠΈΠ²Π°ΡΡΠ΅ΠΉ ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄ΡΡΠ²ΠΎ Π½Π΅ΠΏΡΠΎΠ²ΠΈΡΠ°ΡΡΠ΅ΠΉ Π²ΠΎΠ»ΡΡΡΠ°ΠΌΠΎΠ²ΠΎΠΉ ΠΏΡΠΎΠ²ΠΎΠ»ΠΎΠΊΠΈ
Research of Mechanism and Kinetics of obtaining Tungsten Blue Oxide by Ammonium Paratungstate Tempering
ΠΠ·ΡΡΠ΅Π½Ρ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌ ΠΈ ΠΊΠΈΠ½Π΅ΡΠΈΠΊΠ° ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΡΠΈΠ½Π΅Π³ΠΎ ΠΎΠΊΡΠΈΠ΄Π° Π²ΠΎΠ»ΡΡΡΠ°ΠΌΠ° ΠΏΡΠΎΠΊΠ°Π»ΠΊΠΎΠΉ ΠΏΠ°ΡΠ°Π²ΠΎΠ»ΡΡΡΠ°ΠΌΠ°ΡΠ°
Π°ΠΌΠΌΠΎΠ½ΠΈΡ Π² Β«Π°Π²ΡΠΎΠ³Π΅Π½Π½ΠΎΠΉΒ» Π°ΡΠΌΠΎΡΡΠ΅ΡΠ΅. ΠΡΠ΅Π΄Π»ΠΎΠΆΠ΅Π½Ρ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΡΠ΅ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΡ ΠΏΡΠΎΡΠ΅ΡΡΠ°.Mechanism and kinetics of obtaining tungsten blue oxide by ammonium paratungstate tempering in
autogenous atmosphere are researched. Optimal process characteristics proposed
Research of Mechanism and Kinetics of obtaining Tungsten Blue Oxide by Ammonium Paratungstate Tempering
ΠΠ·ΡΡΠ΅Π½Ρ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌ ΠΈ ΠΊΠΈΠ½Π΅ΡΠΈΠΊΠ° ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΡΠΈΠ½Π΅Π³ΠΎ ΠΎΠΊΡΠΈΠ΄Π° Π²ΠΎΠ»ΡΡΡΠ°ΠΌΠ° ΠΏΡΠΎΠΊΠ°Π»ΠΊΠΎΠΉ ΠΏΠ°ΡΠ°Π²ΠΎΠ»ΡΡΡΠ°ΠΌΠ°ΡΠ°
Π°ΠΌΠΌΠΎΠ½ΠΈΡ Π² Β«Π°Π²ΡΠΎΠ³Π΅Π½Π½ΠΎΠΉΒ» Π°ΡΠΌΠΎΡΡΠ΅ΡΠ΅. ΠΡΠ΅Π΄Π»ΠΎΠΆΠ΅Π½Ρ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΡΠ΅ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΡ ΠΏΡΠΎΡΠ΅ΡΡΠ°.Mechanism and kinetics of obtaining tungsten blue oxide by ammonium paratungstate tempering in
autogenous atmosphere are researched. Optimal process characteristics proposed
Use of Infant Formulas in Obstetric Hospitals
The results of a questionnaire survey of mothers on the use of infant formulas at the obstetric hospital have been presented. Possible ways to improve the situation with the use of infant formulas in term infants at the obstetric hospital have been discussed. A high frequency of the use of formulas in healthy newborns at the obstetric hospital as well as the practice of issuing formulas as a gift on discharge from the obstetric hospital have been revealed. The literature data has been given on the negative effect of both unreasonable use of formulas and the issuance of formulas as a gift on discharge on the preservation of breastfeeding. It is expected that restricting the distribution of milk formulas without proper indications will lead to an increase in the prevalence of breastfeeding
CRISPR-Cas Systems in Gut Microbiome of Children with Autism Spectrum Disorders
The human gut microbiome is associated with various diseases, including autism spectrum disorders (ASD). Variations of the taxonomical composition in the gut microbiome of children with ASD have been observed repeatedly. However, features and parameters of the microbiome CRISPR-Cas systems in ASD have not been investigated yet. Here, we demonstrate such an analysis in order to describe the overall changes in the microbiome CRISPR-Cas systems during ASD as well as to reveal their potential to be used in diagnostics and therapy. For the systems identification, we used a combination of the publicly available tools suited for completed genomes with subsequent filtrations. In the considered data, the microbiomes of children with ASD contained fewer arrays per Gb of assembly than the control group, but the arrays included more spacers on average. CRISPR arrays from the microbiomes of children with ASD differed from the control group neither in the fractions of spacers with protospacers from known genomes, nor in the sets of known bacteriophages providing protospacers. Almost all bacterial protospacers of the gut microbiome systems for both children with ASD and the healthy ones were located in prophage islands, leaving no room for the systems to participate in the interspecies competition
ΠΠ΅ΠΉΡΠΎΡΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΡ Ρ ΠΈΠΌΠΈΠΎΡΠ΅ΡΠ°ΠΏΠΈΠΈ Ρ Π΄Π΅ΡΠ΅ΠΉ. ΠΠ±Π·ΠΎΡ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΡ
The article provides the review of literature on the issue of neurotoxic complications of chemotherapy in children. The data search was carried out in the PubMed, CyberLeninka, RSCI databases and others. There are many works devoted to studying the distribution, structure, and clinical features of chemo-induced neurological disorders in modern literature. In general, analysis of literature has shown that the incidence of neurotoxic complications varies widely depending on the research methods and used chemo-ther-apeutic drugs. Manifestations of chemotherapy-induced neurological disorders are quite diverse and accompanied by the central and peripheral nervous system damage. However, it has been shown that the platinum-based drugs have effective ototoxic effect with the damage of the auditory nerve. The Vincristine often causes peripheral polyneuropathy. Methotrexate and Ifosfamide cause metabolic encephalopathy. Cytarabineβs neurotoxicity is the damage of the cerebellum. The addition of neurological symptoms leads to decrease in the quality of life of patients. Some studies present the results of study of the causes and pathogenetic manifestations of neurotoxic reactions, changes in the genome associated with the development of specific toxic effects are identified. Despite the fact that many pathogenetic mechanisms of chemotherapy-induced neurotoxic complications have been revealed, the significance of laboratory predictors for prognosing and early assessment of these complications has not been essentially established yet, thus, there is the need for further study of this topic.Π ΡΡΠ°ΡΡΠ΅ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½ ΠΎΠ±Π·ΠΎΡ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΡ ΠΏΠΎ ΠΏΡΠΎΠ±Π»Π΅ΠΌΠ΅ Π½Π΅ΠΉΡΠΎΡΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠΉ Ρ
ΠΈΠΌΠΈΠΎΡΠ΅ΡΠ°ΠΏΠΈΠΈ Ρ Π΄Π΅ΡΠ΅ΠΉ, ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ ΠΏΠΎΠΈΡΠΊ ΠΏΠΎ Π±Π°Π·Π°ΠΌ Π΄Π°Π½Π½ΡΡ
PubMed, CyberLeninka, Π ΠΠΠ¦ ΠΈ Π΄Ρ. Π ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΠΉ Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΠ΅ ΠΌΠ½ΠΎΠ³ΠΎ ΡΠ°Π±ΠΎΡ ΠΏΠΎΡΠ²ΡΡΠ΅Π½ΠΎ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½ΠΈΡ, ΡΡΡΡΠΊΡΡΡΡ ΠΈ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΠ°ΡΡΠΈΠ½Ρ Ρ
ΠΈΠΌΠΈΠΎΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π½Π΅Π²ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ°ΡΡΡΡΠΎΠΉΡΡΠ². Π ΡΠ΅Π»ΠΎΠΌ Π°Π½Π°Π»ΠΈΠ· Π»ΠΈΡΠ΅ΡΠ°ΡΡΡΠ½ΡΡ
ΠΈΡΡΠΎΡΠ½ΠΈΠΊΠΎΠ² ΠΏΠΎΠΊΠ°Π·Π°Π», ΡΡΠΎ ΡΠ°ΡΡΠΎΡΠ° Π²ΡΡΡΠ΅ΡΠ°Π΅ΠΌΠΎΡΡΠΈ Π½Π΅ΠΉΡΠΎΡΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠΉ ΡΠΈΡΠΎΠΊΠΎ Π²Π°ΡΡΠΈΡΡΠ΅Ρ Π² Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΎΡ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠΌΡΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΠ΅ΠΌΡΡ
Ρ
ΠΈΠΌΠΈΠΎΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ². ΠΡΠΎΡΠ²Π»Π΅Π½ΠΈΡ Ρ
ΠΈΠΌΠΈΠΎΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π½Π΅Π²ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ°ΡΡΡΡΠΎΠΉΡΡΠ² Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎ ΡΠ°Π·Π½ΠΎΠΎΠ±ΡΠ°Π·Π½Ρ ΠΈ ΡΠΎΠΏΡΠΎΠ²ΠΎΠΆΠ΄Π°ΡΡΡΡ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΠ΅ΠΌ ΡΠ΅Π½ΡΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΈ ΠΏΠ΅ΡΠΈΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π½Π΅ΡΠ²Π½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ. ΠΠ΄Π½Π°ΠΊΠΎ, ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΡ ΠΏΠ»Π°ΡΠΈΠ½Ρ ΠΎΠΊΠ°Π·ΡΠ²Π°ΡΡ ΠΏΡΠ΅ΠΈΠΌΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎ ΠΎΡΠΎΡΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ Ρ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΠ΅ΠΌ ΡΠ»ΡΡ
ΠΎΠ²ΠΎΠ³ΠΎ Π½Π΅ΡΠ²Π°. ΠΡΠΈ Π»Π΅ΡΠ΅Π½ΠΈΠΈ Π°Π»ΠΊΠ°Π»ΠΎΠΈΠ΄Π°ΠΌΠΈ Π±Π°ΡΠ²ΠΈΠ½ΠΊΠ° Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎ ΡΠ°ΡΡΠΎ ΡΠΎΡΠΌΠΈΡΡΠ΅ΡΡΡ ΠΏΠ΅ΡΠΈΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΏΠΎΠ»ΠΈΠ½Π΅ΠΉΡΠΎΠΏΠ°ΡΠΈΡ. ΠΠ° ΡΠΎΠ½Π΅ ΠΏΡΠΈΠ΅ΠΌΠ° ΠΌΠ΅ΡΠΎΡΡΠ΅ΠΊΡΠ°ΡΠ° ΠΈ ΠΈΡΠΎΡΡΠ°ΠΌΠΈΠ΄Π° ΡΠ°Π·Π²ΠΈΠ²Π°Π΅ΡΡΡ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΠ½ΡΠ΅ΡΠ°Π»ΠΎΠΏΠ°ΡΠΈΡ. Π ΠΎΡΠ½ΠΎΠ²Π½ΡΠΌ ΠΏΡΠΎΡΠ²Π»Π΅Π½ΠΈΡΠΌ Π½Π΅ΠΉΡΠΎΡΠΎΠΊΡΠΈΡΠ½ΠΎΡΡΠΈ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ° ΡΠΈΡΠ°ΡΠ°Π±ΠΈΠ½Π° ΠΎΡΠ½ΠΎΡΡΡ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΠ΅ ΠΌΠΎΠ·ΠΆΠ΅ΡΠΊΠ°. ΠΡΠΈΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠ΅ Π½Π΅Π²ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠΈΠΌΠΏΡΠΎΠΌΠΎΠ² ΡΠ½ΠΈΠΆΠ°Π΅Ρ ΠΊΠ°ΡΠ΅ΡΡΠ²ΠΎ ΠΆΠΈΠ·Π½ΠΈ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ², ΡΡΡΠ³ΡΠ±Π»ΡΠ΅Ρ ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠ³ΠΎ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ ΠΈ Π²Π»ΠΈΡΠ΅Ρ Π½Π° ΠΏΡΠΎΠ³Π½ΠΎΠ·. Π Π½Π΅ΠΊΠΎΡΠΎΡΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡΡ
ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Ρ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΠΏΡΠΈΡΠΈΠ½ ΠΈ ΠΏΠ°ΡΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΠΎΠ² Π½Π΅ΠΉΡΠΎΡΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ΅Π°ΠΊΡΠΈΠΉ, Π²ΡΡΠ²Π»Π΅Π½Ρ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π³Π΅Π½ΠΎΠΌΠ°, ΡΠ²ΡΠ·Π°Π½Π½ΡΠ΅ Ρ ΡΠΈΡΠΊΠΎΠΌ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΡΡΠ΅ΠΊΡΠΎΠ². ΠΠ΅ΡΠΌΠΎΡΡΡ Π½Π° ΡΠΎ, ΡΡΠΎ ΠΌΠ½ΠΎΠ³ΠΈΠ΅ ΠΏΠ°ΡΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΡ Ρ
ΠΈΠΌΠΈΠΎΠΈΠ½Π΄ΡΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π½Π΅ΠΉΡΠΎΡΠΎΠΊΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠΉ ΡΠ°ΡΡΠΈΡΡΠΎΠ²Π°Π½Ρ, Π·Π½Π°ΡΠ΅Π½ΠΈΠ΅ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½ΡΡ
ΠΏΡΠ΅Π΄ΠΈΠΊΡΠΎΡΠΎΠ² Π΄Π»Ρ ΠΏΡΠΎΠ³Π½ΠΎΠ·ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΈ ΡΠ°Π½Π½Π΅ΠΉ ΠΎΡΠ΅Π½ΠΊΠΈ ΡΡΠΈΡ
ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠΉ Π½Π΅ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ΅Ρ Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½ΠΈΠ΅ Π΄Π°Π»ΡΠ½Π΅ΠΉΡΠ΅Π³ΠΎ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ Π΄Π°Π½Π½ΠΎΠΉ ΠΏΡΠΎΠ±Π»Π΅ΠΌΡ