Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824.

Background: Consolidated bioprocessing (CBP) is reliant on the simultaneous enzyme production, saccharification of biomass, and fermentation of released sugars into valuable products such as butanol. Clostridial species that produce butanol are, however, unable to grow on crystalline cellulose. In contrast, those saccharolytic species that produce predominantly ethanol, such as Clostridium thermocellum and Clostridium cellulolyticum, degrade crystalline cellulose with high efficiency due to their possession of a multienzyme complex termed the cellulosome. This has led to studies directed at endowing butanol-producing species with the genetic potential to produce a cellulosome, albeit by localising the necessary transgenes to unstable autonomous plasmids. Here we have explored the potential of our previously described Allele-Coupled Exchange (ACE) technology for creating strains of the butanol producing species Clostridium acetobutylicum in which the genes encoding the various cellulosome components are stably integrated into the genome. Results: We used BioBrick2 (BB2) standardised parts to assemble a range of synthetic genes encoding C. thermocellum cellulosomal scaffoldin proteins (CipA variants) and glycoside hydrolases (GHs, Cel8A, Cel9B, Cel48S and Cel9K) as well as synthetic cellulosomal operons that direct the synthesis of Cel8A, Cel9B and a truncated form of CipA. All synthetic genes and operons were integrated into the C. acetobutylicum genome using the recently developed ACE technology. Heterologous protein expression levels and mini-cellulosome self-assembly were assayed by western blot and native PAGE analysis. Conclusions: We demonstrate the successful expression, secretion and self-assembly of cellulosomal subunits by the recombinant C. acetobutylicum strains, providing a platform for the construction of novel cellulosomes. © 2013 Kovács et al.; licensee BioMed Central Ltd

Margin maximizing discriminant analysis

Abstract. We propose a new feature extraction method called Margin Maximizing Discriminant Analysis (MMDA) which seeks to extract features suitable for classification tasks. MMDA is based on the principle that an ideal feature should convey the maximum information about the class labels and it should depend only on the geometry of the optimal decision boundary and not on those parts of the distribution of the input data that do not participate in shaping this boundary. Further, distinct feature components should convey unrelated information about the data. Two feature extraction methods are proposed for calculating the parameters of such a projection that are shown to yield equivalent results. The kernel mapping idea is used to derive non-linear versions. Experiments with several real-world, publicly available data sets demonstrate that the new method yields competitive results.

Far-Infrared Properties of Spitzer-selected Luminous Starbursts

We present SHARC-2 350 micron data on 20 luminous z ~ 2 starbursts with S(1.2mm) > 2 mJy from the Spitzer-selected samples of Lonsdale et al. and Fiolet et al. All the sources were detected, with S(350um) > 25 mJy for 18 of them. With the data, we determine precise dust temperatures and luminosities for these galaxies using both single-temperature fits and models with power-law mass--temperature distributions. We derive appropriate formulae to use when optical depths are non-negligible. Our models provide an excellent fit to the 6um--2mm measurements of local starbursts. We find characteristic single-component temperatures T1 ~ 35.5+-2.2 K and integrated infrared (IR) luminosities around 10^(12.9+-0.1) Lsun for the SWIRE-selected sources. Molecular gas masses are estimated at 4 x 10^(10) Msun, assuming kappa(850um)=0.15 m^2/kg and a submillimeter-selected galaxy (SMG)-like gas-to-dust mass ratio. The best-fit models imply >~2 kpc emission scales. We also note a tight correlation between rest-frame 1.4 GHz radio and IR luminosities confirming star formation as the predominant power source. The far-IR properties of our sample are indistinguishable from the purely submillimeter-selected populations from current surveys. We therefore conclude that our original selection criteria, based on mid-IR colors and 24 um flux densities, provides an effective means for the study of SMGs at z ~ 1.5--2.5.Comment: 13 pages, 4 figures, edited to match published version in ApJ 717, 29-39 (2010

The antiepileptic potential of nucleosides

Despite newly developed antiepileptic drugs to suppress epileptic symptoms, approximately one third of patients remain drug refractory. Consequently, there is an urgent need to develop more effective therapeutic approaches to treat epilepsy. A great deal of evidence suggests that endogenous nucleosides, such as adenosine (Ado), guanosine (Guo), inosine (Ino) and uridine (Urd), participate in the regulation of pathomechanisms of epilepsy. Adenosine and its analogues, together with non-adenosine (non-Ado) nucleosides (e.g., Guo, Ino and Urd), have shown antiseizure activity. Adenosine kinase (ADK) inhibitors, Ado uptake inhibitors and Ado-releasing implants also have beneficial effects on epileptic seizures. These results suggest that nucleosides and their analogues, in addition to other modulators of the nucleoside system, could provide a new opportunity for the treatment of different types of epilepsies. Therefore, the aim of this review article is to summarize our present knowledge about the nucleoside system as a promising target in the treatment of epilepsy

Overtone and multi-mode RR Lyrae stars in the globular cluster M3

The overtone and multi-mode RR Lyrae stars in the globular cluster M3 are studied using a 200-d long, $B,V$ and $I_{\mathrm C}$ time-series photometry obtained in 2012. 70\% of the 52 overtone variables observed show some kind of multi-periodicity (additional frequency at ${f_{0.61}}={f_{\mathrm {1O}}}/0.61$ frequency ratio, Blazhko effect, double/multi-mode pulsation, period doubling). A signal at 0.587 frequency ratio to the fundamental-mode frequency is detected in the double-mode star, V13, which may be identified as the second radial overtone mode. If this mode-identification is correct, than V13 is the first RR Lyrae star showing triple-mode pulsation of the first three radial modes. Either the Blazhko effect or the ${f_{0.61}}$ frequency (or both of these phenomena) appear in 7 double-mode stars. The $P_{\mathrm{1O}}/P_{\mathrm{F}}$ period ratio of RRd stars showing the Blazhko effect are anomalous. A displacement of the main frequency component at the fundamental-mode with the value of modulation frequency (or its half) is detected in three Blazhko RRd stars parallel with the appearance of the overtone-mode pulsation. The ${f_{0.61}}$ frequency appears in RRc stars that lie at the blue side of the double-mode region and in RRd stars, raising the suspicion that its occurrence may be connected to double-mode pulsation. The changes of the Blazhko and double-mode properties of the stars are also reviewed using the recent and archive photometric data.Comment: accepted for publication in ApJ Suppl. 26 pages, 25 figure

Receptors of peptides as therapeutic targets in epilepsy research

Neuropeptides are signaling molecules participating in the modulation of synaptic transmission. Neuropeptides are stored in dense core synaptic vesicles, the release of which requires profound excitation. Only in the extracellular space, neuropeptides act on G-protein coupled receptors to exert a relatively slow action both pre- and postsynaptically. Consequently, neuropeptide modulators are ideal candidates to influence epileptic tissue overexcited during seizures. Indeed, a number of neuropeptides have receptors implicated in epilepsy and many of them are considered to participate in endogenous neuroprotective actions. Neuropeptide receptors, present in the hippocampus, the most frequent focus of seizures in temporal lobe epilepsy, received the largest attention as potential anti-epileptic targets. Receptors of hippocampal neuropeptides, somatostatin, neuropeptide Y, galanin, dynorphin, enkephalin, substance P, cholecystokinin, vasoactive intestinal polypeptide, and receptors of some neuropeptides, which are also hormones such as ghrelin, angiotensins, corticotropin- releasing hormone, adrenocorticotropin, thyrotropin-releasing hormone, oxytocin and vasopressin involved in epilepsy are discussed in the review article. Activation and inhibition of receptors by oral application of peptides as drugs is typically not efficient because of low bioavailability: rapid degradation and insufficient penetration of peptides through the blood-brain barrier. Recent progress in the development of non-peptide agonists and antagonists of neuropeptide receptors as well as gene therapeutic approaches leading to the local production of agonists and antagonists within the central nervous system will also be discussed

HAT-P-55b: A Hot Jupiter Transiting a Sun-like Star

We report the discovery of a new transiting extrasolar planet, HAT-P-55b. The planet orbits a V = 13.207 +/- 0.039 sun-like star with a mass of 1.013 +/- 0.037 solar masses, a radius of 1.011 +/- 0.036 solar radii and a metallicity of -0.03 +/- 0.08. The planet itself is a typical hot Jupiter with a period of 3.5852467 +/- 0.0000064 days, a mass of 0.582 +/- 0.056 Jupiter masses and a radius of 1.182 +/- 0.055 Jupiter radii. This discovery adds to the increasing sample of transiting planets with measured bulk densities, which is needed to put constraints on models of planetary structure and formation theories.Comment: 7 pages, 4 figures, accepted for publication in PAS