Дифференциально−диагностические нейрофизиологические корреляты эмоционально−личностных и поведенческих расстройств у подростков с мозговой дисфункцией

Представлены результаты исследования функциональной активности головного мозга у подростков с мозговой дисфункцией и эмоциональными, личностными, поведенческими расстройствами. Выявлены нейрофизиологические корреляты изменений характера и поведения больных подростков.The findings of brain functional activity investigation in teenagers with cerebral dysfunction as well as emotional, personality, behavioral disorders are reported. Neurophysiological correlates of the character and behavior changes in teen−agers were revealed

Evaluation of Microsatellite Typing, ITS Sequencing, AFLP Fingerprinting, MALDI-TOF MS, and Fourier-Transform Infrared Spectroscopy Analysis of<i> Candida auris</i>

Candida auris is an emerging opportunistic yeast species causing nosocomial outbreaks at a global scale. A few studies have focused on the C. auris genotypic structure. Here, we compared five epidemiological typing tools using a set of 96 C. auris isolates from 14 geographical areas. Isolates were analyzed by microsatellite typing, ITS sequencing, amplified fragment length polymorphism (AFLP) fingerprint analysis, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS), and Fourier-transform infrared (FTIR) spectroscopy methods. Microsatellite typing grouped the isolates into four main clusters, corresponding to the four known clades in concordance with whole genome sequencing studies. The other investigated typing tools showed poor performance compared with microsatellite typing. A comparison between the five methods showed the highest agreement between microsatellite typing and ITS sequencing with 45% similarity, followed by microsatellite typing and the FTIR method with 33% similarity. The lowest agreement was observed between FTIR spectroscopy, MALDI-TOF MS, and ITS sequencing. This study indicates that microsatellite typing is the tool of choice for C. auris outbreak investigations. Additionally, FTIR spectroscopy requires further optimization and evaluation before it can be used as an epidemiological typing method, comparable with microsatellite typing, as a rapid method for tracing nosocomial fungal outbreaks

Treatment Considerations for Mandibulectomy Patients

Prosthetic rehabilitation of patients after resection of the mandible due to operation of malignant tumors usually poses a great problem. Loss of tissues and damage caused by radiotherapy cause various functional deficiencies and dysfunction in the stomatognathic system. The study concerned treatment of a group of mandibulectomy patients with problems related mainly to restoration of jaw relationship, lack of occlusion and dysfunctions. In the escamined cases immediate or delayed reconstructive surgery had been completed before prosthetics to treat mandibular discontinuity defects. Unfortunately, many of the patients exibit lack of occlusion, mandibular deviations and torque due to incorrect muscle activity. Prosthetic management was part of a multidisciplinary approach to the problem. Treatment included myotherapy, gradual occlusal rearrangement with the use of therapeutic and corrective splints, special appliances and prostheses with leading inclined planes and guiding surfaces. The degree of success was related to the location and extent of the mandibular resection, the shape of the bone transplants and presence or absence of natural teeth. The aims of treatment realised were the restoration of acceptable occlusion and improved functional efficiency of the masticatory system

Recent trends in molecular diagnostics of yeast infections : from PCR to NGS

The incidence of opportunistic yeast infections in humans has been increasing over recent years. These infections are difficult to treat and diagnose, in part due to the large number and broad diversity of species that can underlie the infection. In addition, resistance to one or several antifungal drugs in infecting strains is increasingly being reported, severely limiting therapeutic options and showcasing the need for rapid detection of the infecting agent and its drug susceptibility profile. Current methods for species and resistance identification lack satisfactory sensitivity and specificity, and often require prior culturing of the infecting agent, which delays diagnosis. Recently developed high-throughput technologies such as next generation sequencing or proteomics are opening completely new avenues for more sensitive, accurate and fast diagnosis of yeast pathogens. These approaches are the focus of intensive research, but translation into the clinics requires overcoming important challenges. In this review, we provide an overview of existing and recently emerged approaches that can be used in the identification of yeast pathogens and their drug resistance profiles. Throughout the text we highlight the advantages and disadvantages of each methodology and discuss the most promising developments in their path from bench to bedside

Perinatal Environmental Tobacco Smoke Exposure in Rhesus Monkeys: Critical Periods and Regional Selectivity for Effects on Brain Cell Development and Lipid Peroxidation

Perinatal environmental tobacco smoke (ETS) exposure in humans elicits neurobehavioral deficits. We exposed rhesus monkeys to ETS during gestation and through 13 months postnatally, or postnatally only (6–13 months). At the conclusion of exposure, we examined cerebrocortical regions and the midbrain for cell damage markers and lipid peroxidation. For perinatal ETS, two archetypal patterns were seen in the various regions, one characterized by cell loss (reduced DNA concentration) and corresponding increases in cell size (increased protein/DNA ratio), and a second pattern suggesting replacement of larger neuronal cells with smaller and more numerous glia (increased DNA concentration, decreased protein/DNA ratio). The membrane/total protein ratio, a biomarker of neurite formation, also indicated potential damage to neuronal projections, accompanied by reactive sprouting. When ETS exposure was restricted to the postnatal period, the effects were similar in regional selectivity, direction, and magnitude. These patterns resemble the effects of prenatal nicotine exposure in rodent and primate models. Surprisingly, perinatal ETS exposure reduced the level of lipid peroxidation as assessed by the concentration of thiobarbituric acid reactive species, whereas postnatal ETS did not. The heart, a tissue that, like the brain, has high oxygen demand, displayed a similar but earlier decrease (2–3 months) in lipid peroxidation in the perinatal exposure model, whereas values were reduced at 13 months with the postnatal exposure paradigm. Our results provide a mechanistic connection between perinatal ETS exposure and neurobehavioral anomalies, reinforce the role of nicotine in these effects, and buttress the importance of restricting or eliminating ETS exposure in young children

Gaia transients in galactic nuclei

The high spatial resolution and precise astrometry and photometry of the Gaia mission should make it particularly apt at discovering and resolving transients occurring in, or near, the centres of galaxies. Indeed, some nuclear transients are reported by the Gaia Science Alerts (GSA) team, but not a single confirmed Tidal Disruption Event has been published. In order to explore the sensitivity of GSA, we performed an independent and systematic search for nuclear transients using Gaia observations. Our transient search is driven from an input galaxy catalogue (derived from the Sloan Digital Sky Survey Release 12). We present a candidate detection metric which is independent from the existing GSA methodology, to see if Gaia Alerts are biased against the discovery of nuclear transients, and in particular which steps may have an impact. Our technique does require significant manual vetting of candidates, making implementation in the GSA system impractical for daily operations, although it could be run weekly, which for month-to-year long transients would make a scientifically valuable addition. Our search yielded ~480 nuclear transients, 5 of which were alerted and published by GSA. The list of (in some cases ongoing) transients includes candidates for events related to enhanced accretion onto a super-massive black hole and TDEs. An implementation of the detection methodology and criteria used in this paper as an extension of GSA could open up the possibility for Gaia to fulfil the role as a main tool to find transient nuclear activity as predicted in the literature.Comment: MNRAS accepted; full Table 2 attache