Principle component analysis of equilibrium headspace concentration of beverage emulsion as function of main emulsion components.

A three-factor central composite design (CCD) was used to study the effect of two hydrocolloids namely Arabic gum (7-13% w/w), xanthan gum (0.1-0.3% w/w) as well as orange oil (6-10% w/w) on the equilibrium headspace concentration of target flavor compounds released from the diluted beverage emulsion. Headspace solid phase microextraction (HS-SPME) coupled to gas chromatography (GC) equipped with Time-of-Flight Mass Spectrometer (TOFMS) was employed for the equilibrium headspace analysis of orange beverage emulsion. In qualitative analysis of cold pressed orange oil, 24 volatile flavor compounds were detected by using HS-SPME-GC-TOFMS. Among these volatile flavor compounds, 13 volatile compounds from different chemical classes namely monoterpene hydrocarbons (i.e. α-pinene, β-pinene, 3-carene, myrcene, limonene and γ- terpinene), esters (i.e. ethyl acetate and ethyl butyrate), alcohol (i.e. linalool) and aldehyde compounds (i.e. octanal, decanal, neral and geranial) were composed of >98% of total volatile flavor compounds. For equilibrium headspace analysis, the peak area of target volatile compounds was investigated as response variables. The significant (p < 0.05) second-order regression models with relatively high R2 (≥ 0.785) were fitted for explaining the equilibrium headspace concentration. Orange beverage emulsion containing 7% (w/w) Arabic gum, 0.24% (w/w) xanthan gum and 10% (w/w) orange oil was estimated to provide the highest concentration of equilibrium volatile headspace. Closeness between experimental and predicted equilibrium headspace concentrations indicated the adequacy of the response surface models fitted to the experimental data. Principle component analysis discriminated the beverage emulsions containing the same orange oil content but different contents of emulsifier fraction in different groups, thus indicating significant (p < 0.05) effect of emulsifier fraction on equilibrium headspace concentration of diluted beverage emulsion

Valorizacija sporednih proizvoda iz dorade semenske uljane repice

After technological processing of rapeseed significant amounts of useful and useless waste products stand out. The aim of the present study was to investigate the chemical composition, content of glucosinolates, microbiological safety, and presence of mycotoxins and heavy element contents of useful rapeseed by-products which are intended for animal nutrition. Feed components as well as complete mixtures for animal feed must be safe and in accordance with the requirements of the current national regulation. The investigated useful by-products contained significant amounts of proteins (21.80%) and fat (33.78%). As a part of the research, extrusion of the mixture containing rapeseed by-products and maize in the ratio of 50:50% was performed. The process of extrusion was carried out at 130 °C. Based on the obtained results it was noticed that the investigated extruded mixture is a convenient protein-energy supplement suitable for animal feed production with significantly reduced glucosinolate content (from 10.30 to 7.82 µmol/g). It was also observed that the extrusion of feed mixture led to the reduction of the number of microorganisms which ensures safe feedstuff for animal feed production.U tehnološkom postupku prerade semenske uljane repice izdvaja se značajna količina otpadnih produkata. Cilj istraživanja bio je da se ispita hemijski sastav, sadržaj glukozinolata, mikrobiološka ispravnost, prisustvo mikotoksina i teških metala u nusproizvodima koji se dobijaju pri doradi semenske uljane repice, namenjenih proizvodnji hrane za životinje. Potpune smeše za ishranu životinja kao i hraniva moraju biti higijenski ispravne i udovoljiti zahtevima važećeg Pravilnika o kvalitetu hrane za životinje. Na osnovu ispitivanja pomenutih parametara, ustanovljeno je da ispitivani sporedni proizvodi sadrže značajnu količinu proteina (21,80%) i masti (33,78%). U okviru istraživanja izvršeno je ekstrudiranje sporednog proizvoda iz dorade semenske uljane repice sa kukuruzom u odnosu 50:50%. Ekstrudiranje smeše primesa iz dorade semenske uljane repice i kukuruza je sprovedeno na temperaturi od 130 °C. Dobijeno je hranivo zadovoljavajućeg nutritivno-hemijskog profila, uz značajnu redukciju sadržaja glukozinolata za 24,08% (sa 10,30 na 7,82 µmol/g). Na osnovu izvedenih ispitivanja, ustanovljeno je da procesom ekstrudiranja hraniva dolazi do smanjenja broja mikroorganizama čime se obezbeđuje higijenska ispravnost ekstrudata

Multivariate analysis discrimination of various cold-pressed lemon oils from different geographical regions.

The main objective of present study was to investigate the influence of geographical region on the flavour compounds of various cold-pressed lemon oils, i.e. Argentinean, Ivory Cost, Italian, Spanish and Uruguayan. Among the flavor compounds, the major volatile flavour compounds from different chemical classes namely monoterpene hydrocarbons (i.e. limonene, sabinene plus β-pinene, γ-terpinene, myrcene, α-pinene and α-terpinolene), esters (i.e. neryl acetate and geranyl acetate), alcohol (i.e. α-terpineol) and aldehyde compounds (i.e. geranial and neral) were composed of > 90% of total volatile flavor compounds of target cold pressed lemon oils. Uruguayan essential oil had the least concentration level of all major classes of flavor compounds except for alcohol compounds; while the highest content of all chemical classes of flavor compounds identified in this study. The principle component analysis (PCA) allowed us to discriminate various cold-pressed lemon oil based on their origin resource, thereby classify their sensorial attributes

Utjecaj eteričnog ulja bijeloga pelina (Artemisia absinthium) na antioksidativni status tovnih pilića invadiranih oocistama Eimeria spp.

The aim of this study was to investigate the effects of Artemisia absinthium essential oil (AAEO) on the enzymatic activity of superoxide-dismutase (SOD), glutathione-peroxidase (GSHPx), glutathione-reductase (GR), peroxidase (POD) and xanthine-oxidase (XOD) and the content of lipid peroxides (LPx) and glutathione (GSH) in broilers infected with an oocyst mixture of Eimeria tenella, Eimeria mitis and Eimeria necatrix, compared to coccidicide salinomycine. The investigation was carried out on 240 Arbour acres broilers of both sexes. Broilers were distributed completely randomly into four treatment groups: treatment group A was uninfected and untreated; treatment group B was infected and kept untreated; treatment group C preventively received coccidicide salinomycine in a dose of 60 mg/kg of feed and inoculated with an oocyst mixture on the 21st day-of-age; treatment group D received AAEO in their feed in a dose of 3 g/kg and were infected with the oocyst mixture on the 21st day of age. During the study, bloody diarrhoea was observed from the 3rd to 9th day after the challenge. After six days of infection, the most intensive bloody diarrhoea was noticed in the un-medicated treatment group. In order to evaluate the effects of essential oil on poultry coccidiosis induced by Eimeria spp., oocysts per gram of faeces (OPG) were also investigated in all treatment groups. During the experiment, the oocyst output and mortality rate were significantly lower (P<0.05) in the AAEO treatment group (D2) in comparison to the positive control (B), while significant excretion of oocysts was noticed in the faeces of non-treated broilers infected with Eimeria spp. The broilers treated with salinomycin (C2) showed complete reduction of oocysts in their faeces at 30 days of age. The results obtained in this study indicate changes in the content and the activity of the non-enzymatic and enzymatic antioxidative protective systems in blood hemolysates of infected chickens. The positive preventive effects of AAEO, applied in a concentration of 3g/kg of feed, were high on the antioxidative system of erythrocytes. On the basis of the obtained results, it was concluded that AAEO was effective in lowering the intensity of bloody diarrhoea, as well in reducing the oocyst output of the preventively treated and infected broilers; hence it may be used as a prophylactic feed additive. Moreover, AAEO showed an important role in the activation of antioxidative protection systems in infected broilers, which is of great interest since free radicals and lipid peroxides, formed as a result of lower food intake and exhaustion of the organism induced by diarrhoea, could cause cellular membrane damage.U radu je istražen utjecaj eteričnog ulja bijeloga pelina (Artemisia absinthium) (AAEO) na enzimsku aktivnost superoksid dismutaze (SOD), glutation peroksidaze (GSHPx), glutation reduktaze (GR), peroksidaze (POD) i ksantin-oksidaze (XOD), kao i sadržaj lipidnih peroksida (LPx) i glutationa (GSH) u tovnim pilićima inficiranim smjesom oocista Eimeria tenella, Eimeria mitis i Eimeria necatrix u odnosu na kokcidiostatik salinomicin. Istraživanje in vivo provedeno je na 240 pilića Arbor acres, oba spola. Pilići su bili podijeljeni u četiri skupine. Skupina A je bila neinficirana i netretirana (negativna kontrola). Skupina B je bila inficirana i netretirana (pozitivna kontrola). Skupina C je preventivno dobivala kokcidiostatik salinomicin u količini od 60 mg/kg hrane te je bila inokulirana kokcidijama 21. dana starosti. Skupina D je u hrani dobivala AAEO u količini 3 g/kg te je 21. dana starosti bila inficirana mješavinom oocisti Eimeria sp. Tijekom istraživanja krvava dijareja javljala se od 3. do 9. dana nakon infekcije. Šest dana nakon inficiranja, najintezivnija krvava dijareja zapažena je u netretiranih pilića. U cilju procjene djelovanja eteričnog ulja bijeloga pelina na kokcidiozu kod peradi izazvanu Eimeria oocistama, broj oocista po gramu fecesa (OPG) također je bio istražen u svim skupinama. Tijekom pokusa utvrđeno je da je broj oocista i razina smrtnosti bila znatno niža (P<0,05) u AAEO skupini (D2) u usporedbi s pozitivnom kontrolom (B), dok je značajno izlučivanje oocista uočeno u fecesu pilića koji nisu bili tretirani. Pilići koji su dobivali salinomicin (C2) pokazali su potpunu redukciju oocista u fecesu 30. dana starosti. Rezultati dobiveni u ovoj studiji pokazali su promjene u sadržaju i aktivnosti neenzimskih i enzimskih sustava zaštite u krvi inficiranih tovnih pilića. Zapaženi su pozitivni učinci preventivne primjene AAEO u koncentraciji 3 g/kg hrane na antioksidativni sustav eritrocita. Na osnovi dobivenih rezultata zaključeno je da je eterično ulje Artemisia absinthium vrlo učinkovito u smanjenju jačine krvavog proljeva kao i u smanjenju broja oocista u pilića koji su preventivno tretirani s AAEO. Stoga se može zaključiti da eterično ulje bijeloga pelina može biti davano kao profilaktički dodatak hrani za životinje. Također, AAEO ima pozitivan utjecaj na aktiviranje antioksidativnog sustava zaštite u krvi pilića, što je vrlo značajno s obzirom na to da slobodni radikali i lipidni peroksidi, koji nastaju kao rezultat manjeg unosa hrane i iscrpljenosti organizma uzrokovanog dijarejom, mogu izazvati oštećenje stanične membrane

Bioactive Phenolic Compounds from Lingonberry (Vaccinium vitis-idaea L.): Extraction, Chemical Characterization, Fractionation and Cellular Antioxidant Activity

Lingonberries contain high contents of bioactive compounds such as chlorogenic acids and anthocyanins. In addition to radical scavenging and antioxidant activities, these compounds can protect cells from DNA damage. For this reason, lingonberries might be well suited for nutraceuticals or natural biomedicines. To assess these applications, the present study characterized and identified the most effective extract, only consisting of anthocyanins, copigments or a mixture of both, obtained from a lingonberry juice concentrate. An extract was generated by using a XAD-7 column followed by fractionation into anthocyanins and copigments using adsorptive membrane chromatography. After identification of main polyphenols by HPLC–photodiode array–electrospray ionization–tandem mass spectrometry, free radical scavenging activity was analyzed by electron spin resonance spectroscopy using 2,2-diphenyl-1-picrylhydrazyl and galvinoxyl radicals. Furthermore, cyclic voltammetry analyses and the Trolox equivalent antioxidant capacity (TEAC) assay were applied. Finally, the reactive oxygen species (ROS) reducing effects of the lingonberry extract and its fractions were evaluated in HepG2 cells. While the combination of anthocyanins and copigments possessed the highest antioxidant activities, all samples (XAD-7 extract, anthocyanin and copigment fraction) protected cells from oxidative stress. Thus, synergistic effects between phenolic compounds may be responsible for the high antioxidant potential of lingonberries, enabling their use as nutraceuticals

Potencijal pelina (Artemisia absinthium) kao dodatak u hranidbi kunića: utjecaj na kontrolu kokcidioze, antioksidacijski sustav i proizvodna obilježja

The objective of the study was the investigation of dietary inclusion of Artemisia absinthium as a potential anticoccidial drug in rabbit diet, as well as a growth promoter and antioxidant in rabbit nutrition. The in vivo investigation was carried out on 120 male New Zealand white rabbits. The rabbits were distributed into four dietary treatments with ten replicates each. Two levels of whole wormwood plant were used: 100 g/kg (WW100) and 200 g/kg (WW200). The control treatment (C) was basal diet, and one treatment (CR) received chemical coccidiostat robenidine, supplemented in the amount of 6 g/kg. The rabbits were given ad libitum access to water and feed throughout the entire trial period. Feed intake was measured on a daily basis, while body weight was monitored at an individual level during the entire experimental period every seven days, for a total of 77 days. Blood and liver samples were collected for enzyme analyses, where oocyst counts were determined in 1 g of rabbit feces. At the end of the trial, the addition of 200 g/kg of wormwood led to the highest body weight (3047 g), as well to the lowest feed conversion ratio (3.20 kg/kg) with significant differences compared to the control treatment (3.75 kg/kg). The highest recorded glutatione reductase value was with treatment WW100, with significant differences to other experimental treatments. Consumption of coccidiostat and dried wormwood plant in different levels led to a notably lower malondialdehyde content in liver homogenate compared to the control treatment. Finally, the lowest number of oocysts was found in the treatment receiving wormwood in a concentration of 100 g/kg. On the basis of the results achieved, it may be noticed that wormwood supplementation in the diet of rabbits has a positive influence on growth performance, as well on antioxidative systems in rabbits. Subsequently, it was shown that wormwood can successfully be used in reducing the number of oocysts in rabbits.Cilj je ovog rada bio istražiti dodatak pelina (Artemisia absinthium) u hranidbi kunića kao potencijalni antikokcidiostatik, ali i kao promotor rasta i antioksidans. Pokus je izveden in vivo na 120 bijelih novozelandskih kunića muškog spola, a bio je ponovljen četiri puta pri čemu je formirano 10 skupina. Korištene su dvije koncentracije cijele biljke pelina: 100 g/kg (WW100) i 200 g/kg (WW200). Kunići u kontrolnoj skupini (C) bili su hranjeni smjesama bez ikakvih dodataka, dok je u hranidbi pokusnih kunića (CR) dodan kemijski kokcidiostatik robenidin u količini od 6 g/kg. Voda i hrana bili su im dostupni ad libitum tijekom cijelog pokusnog razdoblja. Utrošak hrane bio je mjeren na dnevnom razini, dok je tjelesna težina bila praćena na individualnoj razini tijekom cijelog pokusa svakih sedam dana, ukupno 77 dana. Uzorci krvi i jetre bili su prikupljeni za enzimske analize, dok je broj oocista utvrđen po 1 g fecesa. Na kraju ekperimenta utvrđeno je da je dodatak 200 g/kg pelina doveo do najveće tjelesne mase kunića (3047 g) i do najmanje konverzije hrane (3,20 kg/kg) koja se značajno razlikovala u usporedbi s kontrolom (3,75 kg/kg). Najveća zabilježena vrijednost glutation reduktaze bila je u tretmanu WW100 sa značajnim razlikama u odnosu na druge eksperimentalne tretmane. Upotreba kokcidiostatika i osušene biljke pelina u različitim koncentracijama dovela je do znatno manjeg sadržaja malondialdehida u homogenatu jetre u usporedbi sa kontrolnim tretmanom. Također, najmanji broj oocista bio je zabilježen u tretmanu u kome je pelin dodan u koncentraciji od 100 g/kg. Na osnovi postignutih rezultata, može se primijetiti da dodatak pelina u hranidbi kunića ima pozitivan utjecaj na njihova proizvodna obilježja, kao i na antioksidacijske sustave. Također, ustanovljeno je da se pelin može uspješno koristiti za smanjivanje broja oocista

Soy protein–gum karaya conjugate: emulsifying activity and rheological behavior in aqueous system and oil in water emulsion

The main objective of this study is to investigate the effects of mixing and conjugation of soy protein isolate (SPI) with gum karaya on the characteristics of the hybrid polymer (protein–gum) in both aqueous systems and oil-in-water (O/W) emulsions. It was hypothesized that the covalent linkage of gum karaya with SPI would improve the emulsifying activity and rheological properties of both polymers. Conjugation occurred under controlled conditions (i.e., 60 °C and 75 % relative humidity, 3 days). The conjugated hybrid polymer produced smaller droplet with better uniformity, higher viscosity and stronger emulsifying activity than native gum karaya, suggesting the conjugated polymer provided a bulkier secondary layer with more efficient coverage around oil droplets, thereby inducing stronger resistance against droplet aggregation and flocculation. Emulsions containing the native gum karaya produced the largest droplet size among all prepared emulsions (D 3,2 = 8.6 μm; D 4,3 = 22.4 μm); while the emulsion containing protein–gum conjugate (1:1 g/g) had the smallest droplet size (D 3,2 = 0.2 μm; D 4,3 = 0.7 μm) with lower polydispersity. The protein–gum conjugate (1:1 g/g) also showed the highest elastic and viscous modulus, the lowest polydispersity (span) and the highest emulsifying activity among all native, mixed and conjugated polymers. Therefore, the percentage of gum karaya used for production of O/W emulsion can be decreased by partially replacing it with the conjugated gum

Apiaceae seeds as functional food

The aim of this review was to point to a great importance of plants from Apiaceae family as a functional food. Caraway (Carum carvi L.), anise (Pimpinella anisum L.), coriander (Coriandrum sativum L.), dill (Anethum graveolens L.), fennel (Foeniculum vulgare Mill.) and cumin (Cuminum cyminum L.) are plants from the above-mentioned family whose seeds are widely used in folk medicine, pharmaceutical industry, as spices, flavoring agents and as dietary supplements. These plants are rich in essential oil, which is a mixture of volatile compounds that give it a characteristic aroma. Their antioxidant and antimicrobial activities have been proven and because of these activities they have great potential to be used as natural food conservatives. These plants also have hypoglycemic and hypolipidemic activities as well as anticancer properties. They are used as food supplements in everyday nutrition and as natural health products for the prevention and treatment of many disorders such as inflammations, hyperglycemia, hyperlipidemia and others. Apart from this, these plants have real application in foods such as pastries, meat and dairy products, pickles and salads as well as spice blends like curry powder, garam masala and others

EFFECT OF Artemisia absinthium ESSENTIAL OIL ON ANTIOXIDATIVE SYSTEMS OF BROILER&apos;S LIVER

ABSTRACT: The effect of Artemisia absinthium essential oil (AAEO) on enzymatic activity of superoxide-dismutase (SOD), glutathione-peroxidase (GSHPx), glutathione-reductase (GR), peroxidase (POD), xantine-oxidase (XOD) and non-enzymatic (content of lipid peroxides (LPx) and gluthathione (GSH)) antioxidative status of broilers infected with mixture of oocysts of Eimeria tenella, Eimeria mitis and Eimeria necatrix in comparison to coccidiostat salinomycin was investigated. The in vivo investigation were carried out on 120 Arbor acres broilers of both sexes. Broilers were randomly distributed into four groups. Group A was uninfected and untreated; group B was infected and was kept untreated; group C preventively received coccidiostatic salinomycin in quantity of 60 mg/kg of feed and was inoculated with coccidia species at 21 st day-of-age and group D received in feed AAEO in quantity of 3 g/kg and was infected with Eimeria oocysts at 21 st day-of-age. Livers were collected for the subsequent evaluation of antioxidative status. It was concluded that AAEO added in feed for broilers prevented the development of coccidia oocysts and therefore it can be used as prophylactic feed additive