Control of Aspergillus niger with garlic, onion and leek extracts

Antifungal activity of “Allium” vegetables that is garlic (Allium sativum L.), onion (Allium cepa L.) and leek (Allium porrum L.) were investigated against Aspergillus niger. Minimal inhibitory concentrations (MIC) and minimal fungicidal concentrations (MFC) of aqueous, ethyl alcohol and acetone extracts were determined by disc diffusion and broth dilution methods in the test tubes. Onion extract with ethyl alcohol (275 mg/mL MFC), aqueous garlic extract (325 mg/mL MFC) and aqueous leek extract (900 mg/mL MFC) found the most inhibitory against A. niger

A comparative study about antioxidant activity and phenolic composition of cumin (Cuminum cyminum L.) and coriander (Coriandrum sativum L.)

383-393Apiaceae (Umbelliferae) is a large family which involves approximately 455 genus and 3600 species. Coriander (C. sativum L.) and cumin (C. cyminum L.) are two members of Apiaceae family which commonly used for medicinal purpose due to their antioxidant activity. Since the antioxidants are compounds that prevent the oxidation by neutralizing free radicals, researches about their potential utilizations are of great interest for food science and technology. Due to the consumer preference and the worries about synthetic antioxidant compounds, the food industry shows tendency to use natural antioxidant compounds obtained from plant materials. FDA has recognized more than 150 plants as GRAS, without no limitations intake. Coriander, cumin, anise, fennel, thyme and oregano are some plants found in this list. The focus of this research is to contrast the composition of phenolic profile and antioxidant activity of ethanolic and methanolic extracts of these two medicinal herbs belonging to the Apiaceae family. For this purpose, coriander and cumin were analyzed for phenolic compounds and antioxidant assay. Antioxidant assay analyses were performed by applying cupric reducing antioxidant capacity (CUPRAC), 2,2'-azino-bis3-ethylbenzothiazoline-6-sulphonic acid (ABTS), ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl methods (DPPH)

An unusual and difficult diagnosis of intestinal obstruction: The abdominal cocoon. Case report and review of the literature

Since publication of our article, "An unusual and difficult diagnosis of intestinal obstruction: The abdominal cocoon. Case report and review of the literature." World J Emerg Surg. 2006, 1: 8 we believe that the case mentioned should have been described as a 'peritoneal encapsulation' rather than 'abdominal cocoon' as concluded in the original publication

An unusual and difficult diagnosis of intestinal obstruction: The abdominal cocoon. Case report and review of the literature

Abdominal cocoon is a rare cause of intestinal obstruction. The abdominal cocoon is probably a developmental abnormality, largely asymptomatic, and is found incidentally at laparotomy or autopsy. Pre-operative diagnosis cannot be often made correctly. This rare entity of intestinal obstruction has been described in the whole literature as a thick fibrotic sac covering the small bowel partially or completely. The etiology of abdominal cocoon is unknown and most often it is found in adolescent girls from tropical or subtropical countries. Complete recovery is generally expected after the removal of the membrane surgically. This paper reports a male patient who has had intestinal obstruction symptoms and has per-operatively been diagnosed as abdominal cocoon

The impact of different commercial probiotic cultures with starters on technological, physicochemical and sensorial properties of a traditional yogurt-based appetizer “Cacık”

Izbor odgovarajuće probiotičke kulture neophodan je za održavanje adekvatnog broja živih stanica do potrošnje, budući da postoji potencijalno nepovoljna interakcija između odabranog soja i starter kulture. Ova studija ispitala je prikladnost Cacıka kao potencijalnog nositelja probiotika, uključujući Lactobacillus acidophilus 74-2, Lactobacillus rhamnosus Howaru HN001TM i Bifidobacterium longum BB536 u različitim kombinacijama. Prisutnost tri probiotika uzrokovala je višu postacidifikaciju i kraće vrijeme fermentacije povezano s većim brojem stanica soja L. bulgaricus. Osim određenih kombinacija koje sadrže B. longum BB536, broj pojedinačnih probiotičkih bakterija bio je veći od 6 log g-1 za sve uzorke Cacık s dodatkom probiotika. Rezultati su pokazali da je bakterijska interakcija bila presudna za preživljavanje tijekom 21 dana skladištenja. U uzorcima koji su sadržavali soj B. longum BB536 sam ili u kombinaciji sa sojem L. acidophilus 74-2 zabilježen je pad broja živih stanica za 2,45 log i 1,95 log po g, zbog čega su se ove kombinacije pokazale neuspješnima u smislu kreiranja probiotičkog proizvoda. U tri od ukupno četiri uzorka koji su sadržavali soj L. rhamnosus Howaru HN001TM sam ili u kombinaciji s drugim probiotičkim sojevima, broj živih stanica L. rhamnosus Howaru HN001TM održao se na početnoj inokuliranoj razini, dok se značajno povećao u ko-kulturi s L. acidophilus 74-2. Uzimajući u obzir ukupna senzorska svojstva i preživljavanje probiotika, Cacık s dodatkom pojedinačnih sojeva L. rhamnosus Howaru HN001TM ili B. longum BB536 i L. rhamnosus Howaru HN001TM ili L. acidophilus 74-2 i L. rhamnosus Howaru HN001TM ili sva tri probiotika može se smatrati pogodnim za daljnju proizvodnju.The selection of proper probiotic culture is essential for maintaining adequate numbers of viable cells until consumption since potential adverse interaction between selected strain and starter culture. This study examined the suitability of Cacık as a potential probiotic carrier including Lactobacillus acidophilus 74-2, Lactobacillus rhamnosus Howaru HN001TM and Bifidobacterium longum BB536 in different combinations. The presence of three probiotic strains caused higher post acidification and shorter fermentation time associated to higher counts of L. bulgaricus. Except for certain combinations containing B. longum BB536, the counts of individual probiotic bacteria were higher than 6 log g-1 for all probiotic supplemented Cacık samples. Results showed that bacterial interaction was decisive for survival over 21 days of storage. Probiotic products containing only B. longum BB536 or B. longum BB536 with L. acidophilus 74-2 did not accomplish in terms of the technological point, since the viable cell counts decerased for 2.45 log and 1.95 log per g, respecitevly, throughout the storage period. In three of totally four combinations included L. rhamnosus Howaru HN001TM alone or combined with other probiotic bacteria, the viable cell counts of L. rhamnosus Howaru HN001TM remained at the inoculated level, while the counts significantly increased in co-culture with L. acidophilus 74-2. Considering the overall sensorial attributes and survival of probiotics, Cacık supplemented with L. rhamnosus Howaru HN001TM alone, combinations of B. longum BB536 and L. rhamnosus Howaru HN001TM or L. acidophilus 74-2 and L. rhamnosus Howaru HN001TM or by all of the three tested probiotics, were suggested as suitable for further production

The effects of Stanozolol and high protein diet on Colon Anastomosis

Background: Anastomotic leakage after colonic resection and anastomosis are serious complications with high morbidity and mortality. Collagen synthesis is an essential feature of anastomotic healing. Objective: Our study aimed to investigate individual and combined effects of high protein diet and Stanozolol on the intestinal anastomosis in rats. Methods: Total of 48 Wistar-albino rats were divided into four groups. Group C: Control group; Group P: high protein diet (4mg/day) administered by gavage; Group A: group to which stanozolol was administered intramuscularly (i.m) (2mg/kg/day); Group A+P: group to which both stanozolol (2mg/kg/day) i.m and high protein diet (4mg/day by gavage) was administered. In each group, half of the animals underwent surgery on the 3rd postoperative (PO) day and the other half on the 7th PO day for analysis procedures. Bursting pressures of each anastomotic segment, tissue hydroxyproline levels, and histologic tissue repair process were evaluated. Results: On 7th PO day, group A+P had statistically and significantly higher bursting pressures than the control group. Hydroxyproline levels at the anastomotic segment on both 3rd and 7th PO days were also significantly higher in group A+P. Conclusion: A high protein diet and stanozolol do not significantly impact the anastomotic healing when used separately. We concluded that high protein diet and Stanozolol can be used after colorectal surgery in humans but requires further research

A five-year perspective on the situation of haemorrhagic fever with renal syndrome and status of the hantavirus reservoirs in Europe, 2005-2010

Hantavirus infections are reported from many countries in Europe and with highly variable annual case numbers. In 2010, more than 2,000 human cases were reported in Germany, and numbers above the baseline have also been registered in other European countries. Depending on the virus type human infections are characterised by mild to severe forms of haemorrhagic fever with renal syndrome. The member laboratories of the European Network for diagnostics of Imported Viral Diseases present here an overview of the progression of human cases in the period from 2005 to 2010. Further we provide an update on the available diagnostic methods and endemic regions in their countries, with an emphasis on occurring virus types and reservoirs

European survey on laboratory preparedness, response and diagnostic capacity for crimean-congo haemorrhagic fever, 2012

Crimean-Congo haemorrhagic fever (CCHF) is an infectious viral disease that has (re-)emerged in the last decade in south-eastern Europe, and there is a risk for further geographical expansion to western Europe. Here we report the results of a survey covering 28 countries, conducted in 2012 among the member laboratories of the European Network for Diagnostics of 'Imported' Viral Diseases (ENIVD) to assess laboratory preparedness and response capacities for CCHF. The answers of 31 laboratories of the European region regarding CCHF case definition, training necessity, biosafety, quality assurance and diagnostic tests are presented. In addition, we identifi

Specialist laboratory networks as preparedness and response tool - The emerging viral diseases-expert laboratory network and the chikungunya outbreak, Thailand, 2019

We illustrate the potential for specialist laboratory networks to be used as preparedness and response tool through rapid collection and sharing of data. Here, the Emerging Viral Diseases-Expert Laboratory Network (EVD-LabNet) and a laboratory assessment of chikungunya virus (CHIKV) in returning European travellers related to an ongoing outbreak in Thailand was used for this purpose. EVD-LabNet rapidly collected data on laboratory requests, diagnosed CHIKV imported cases and sequences generated, and shared among its members and with the European Centre for Disease Prevention and Control. Data across the network showed an increase in CHIKV imported cases during 1 October 2018-30 April 2019 vs the same period in 2018 (172 vs 50), particularly an increase in cases known to be related to travel to Thailand (72 vs 1). Moreover, EVD-LabNet showed that strains were imported from Thailand that cluster with strains of the ECSA-IOL E1 A226 variant emerging in Pakistan in 2016 and involved in the 2017 outbreaks in Italy. CHIKV diagnostic requests increased by 23.6% between the two periods. The impact of using EVD-LabNet or similar networks as preparedness and response tool could be improved by standardisation of the collection, quality and mining of data in routine laboratory management systems