Study Of Lactic Acid Thermal Behavior Using Thermoanalytical Techniques

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Actually, there is a growing interest in the biotechnological production of lactic acid by fermentation aiming to substitute fossil fuel routes. The development of an efficient method for its separation and purification from fermentation broth is very important to assure the economic viability of production. Due to its high reactivity and tendency to decompose at high temperatures, the study of lactic acid thermal behavior is essential for its separation processes and potential application. In the present study, differential scanning calorimetry (DSC) analyses showed endothermic peaks related to the process of evaporation. Data of thermogravimetry (TG/DTG) were correlated to Arrhenius and Kissinger equations to provide the evaporation kinetic parameters and used to determine the vaporization enthalpy. Activation energies were 51.08 and 48.37 kJ.mol(-1) and frequency values were 859.97 and 968.81s(-1) obtained by Arrhenius and Kissinger equations, respectively. Thermogravimetry, coupled with mass spectroscopy (TG-MS), provided useful information about decomposition products when lactic acid was heated at 573 K for approximately 30 min.Sao Paulo Research Foundation (FAPESP) [2012/17501-0, 2015/12783-5]Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Separation and purification strategies for lactic acid produced by fermentation

Orientadores: Maria Regina Wolf Maciel, Maciel, Rubens Maciel FilhoTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia QuímicaResumo: O desenvolvimento de processos biotecnológicos industriais que utilizem recursos naturais renováveis é uma necessidade que se faz presente nos dias atuais, dada a preocupação com o abastecimento de petróleo e o desenvolvimento sustentável. Um grande interesse pelos processos fermentativos para produção de ácidos carboxílicos a partir de recursos renováveis tem sido despertado, sendo a produção de ácido láctico uma das mais importantes entre os ácidos orgânicos. A grande variedade de aplicações e o desenvolvimento de novos usos e produtos, como na produção de polímeros biodegradáveis (poli- ácido láctico), solventes verdes e químicos oxigenados, fizeram com que a produção de ácido láctico crescesse consideravelmente nas últimas décadas e estimulasse a pesquisa por tecnologias que tornem o processo mais viável economicamente. No caso do ácido láctico, o desenvolvimento de um método eficaz de separação e purificação do ácido a partir do caldo de cana-de-açúcar fermentado, sem a geração de efluentes, é de suma importância, pois o processo de separação e purificação corresponde a, aproximadamente, 50% do custo de produção. Assim, este trabalho teve como objetivo estudar três estratégias para separação e concentração do ácido láctico produzido por via fermentativa, utilizando, a saber: um sistema híbrido de destilação molecular, um sistema de destilação reativa e acoplando as duas tecnologias sequencialmente. Foram aplicados planejamentos experimentais para a determinação das melhores condições operacionais de cada processo e, por fim, foram definidas as melhores condições e sequências operacionais para a concentração, separação e purificação do ácido láctico. Os resultados mostraram que utilizar tanto o sistema híbrido de destilação molecular quanto o sistema de destilação reativa são efetivos para a separação e concentração do ácido láctico. Após a otimização da etapa de destilação molecular híbrida, foi possível a obtenção de ácido láctico com 89,71% de pureza. Em relação à destilação reativa, foi possível obter 100% de rendimento de lactato de etila na etapa de esterificação e concentrar 2,4 vezes o ácido láctico, com 26,32% de pureza, após a etapa de hidrólise. Utilizando a destilação molecular híbrida e destilação reativa em sequência, obteve-se ácido láctico 4,7 vezes mais concentrado, com 21,97% de pureza. A destilação molecular híbrida apresentou o maior índice de desempenho de purificação, sendo a tecnologia de separação e purificação mais promissora para o ácido láctico. Para finalizar o trabalho, foi desenvolvida a simulação de uma planta virtual para realizar a purificação do ácido láctico proveniente da fermentação utilizando a destilação reativaAbstract: The development of industrial biotechnology processes that use renewable resources is a necessity nowadays due to concerns about oil supply and sustainable development. Fermentation processes for the production of carboxylic acids from renewable resources have drawing a lot of interest, and the production of lactic acid is one of the most important among organic acids. The wide variety of applications and the development of new uses and products, such as the production of biodegradable polymers, green solvents and oxygenated chemicals, have made the production of lactic acid grow considerably in recent decades, stimulating research for technologies that make the process more economically viable. In the case of lactic acid produced from sugarcane molasses, the development of an effective method of separation and purification without wastewater generation is extremely important. The process of separation and purification corresponds to approximately 50% of the production cost. Therefore, this work is devoted to study three strategies of separation to concentrate lactic acid produced from fermentation: hybrid short path evaporation, reactive distillation, and both technologies attached. Experimental factorial designs were applied to determine the best operating conditions. Thus, the best conditions and operational sequences for lactic acid concentration, separation, and purification were defined. The results showed that hybrid short path evaporation and reactive distillation system are effective for the lactic acid separation and concentration. After optimization of the hybrid short path distillation, lactic acid purity around 89,71% was obtained. Regarding reactive distillation, it was possible to obtain 100% ethyl lactate yield in the esterification reaction and concentrate lactic acid by 2,4 times after hydrolysis with lactic acid purity around 26,32%. Using both technologies attached, lactic acid was concentrated by 4,7 times with lactic acid purity around 21,97%. Hybrid short path distillation showed high purification performance index, so it is the separation and purification technology most promising for lactic acid. Finally, a virtual plant for lactic acid purification from fermentation broth was developed using reactive distillationDoutoradoDesenvolvimento de Processos QuímicosDoutora em Engenharia Quimic

Separation and purification technologies for lactic acid – a brief review

Lactic acid is an important platform chemical with a wide range of applications. Production of lactic acid by fermentation is advantageous because renewable and low cost raw materials can be used as substrates. After fermentation, the broth needs to be purified to obtain pure lactic acid for further uses. Thus, efficient downstream processes are very important because they account for 50% of the production costs. This review discusses different processes that are currently employed for lactic acid recovery, focusing on precipitation, solvent extraction, and separation with membranes. Advances in such recovery processes and drawbacks that limit the application of these technologies at the industrial level are also presented12368856901FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP2015/12783-

Hydrothermal pretreatment for enhancing enzymatic hydrolysis of seeds of açaí (Euterpe oleracea) and sugar recovery

The açaí seeds are an abundant byproduct of the chain of açaí and represent a significant source of lignocellulosic material for bioethanol production in the northern region of Brazil. In the present paper seeds of açaí were hydrothermally treated to overcome biomass recalcitrance for enzymatic hydrolysis. The effect of the hydrothermal pretreatment carried out with high solid loadings in the rates of sugars released was evaluated by subsequent enzymatic hydrolysis. The pretreatment used acid concentrations ranging from (0.5-1.5 %), solids loadings from 5 to 15 % (w/v) and time from 30 to 90 min at 120 °C under a pressure of 1.7 MPa. The enzymatic hydrolysis was carried out at a solid loading of 10 % DM. Hydrolysis was performed at 52 °C and pH 5.0 with an enzyme loading of 30 FPU of celullase and 50 CBU of ß- glycosidase for 72 h. The results showed that hydrothermal treatment can remove significantly hemicelluloses from seeds of açaí (80 %) and improve of yield monosaccharides released by hydrolysis enzymatic. Pre-treatment with 1.5 % sulphuric acid and 5 % solid content for 90 min at 120 °C was found to be the best condition for pre-treatment and conversion37787792International Conference on BioMass (iconBM 2014)sem informaçã

Lactic Acid Purification By Reactive Distillation System Using Design Of Experiments

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)In this study, lactic acid purification using reactive distillation system was evaluated. Factorial experimental design is used to evaluate the influence of parameters and their interactions, such as ethanol/lactic acid molar ratio, reboiler temperature and catalyst concentration on yield of lactate. The results showed that the process for lactic acid purification proposed in this work, provides large potential to achieve high yield of ethyl lactate (-100%) and lactic acid with 3 times higher concentration than the raw material. (C) 2015 Elsevier B.V. All rights reserved.952630Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP [2012/17501-0

Bondeskoler og øvrighedsskoler

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)In this work, the evaluation of lactic acid concentration from a synthetic mixture of water and lactic acid (3 wt% of lactic acid) was carried out using short path evaporation (SPE). The evaporator temperature of a SPE is one of the important technological parameters that determine an evaporator's operation. In this study, the effect of the evaporator temperature on the lactic acid purity and recovery was observed from 303 to 443 K. The results showed that, in the studied range, the evaporator temperature of 353 K can be regarded as the best, since an increase of 2.15 times in the solution concentration was obtained.501015481553Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Challenges and opportunities in lactic acid bioprocess design—From economic to production aspects

Lactic acid is an already consolidated bioproduct in the world market. It has many applications, such as: the production of biodegradable polymers, substitution of plastics from oil, and new uses in medicine. Besides this, new applications are being discovered every year, especially in chemical industries as a building-block molecule. The lactic acid market is in constant growth and the fact that the final products are able to comply with environmental laws as green, renewable and biodegradable products contributes to the tendency of continuous growth in the next few years. With all of this in mind, this paper will explore the main aspects of the sector, as well as market tendencies, production chain, and innovation133219239FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP2013/26290-

Evaluation of alkaline delignification (Naoh) of açaí seeds (Eutherpe Oleracea) treated with H2so4 dilute and effect on enzymatic hydrolysis

Ethanol production from lignocellulosic residues from agriculture, such as the acaí seeds, have a fundamental step, delignification. This step becomes is more effective when performed in combination with others pre- treatments, preferably with those that are able to remove the hemicellulose fraction, such as treatment with dilute sulfuric acid. The aim this work was perform the delignification of açaí seeds pretreated with dilute sulfuric acid and evaluate the enzymatic hydrolysis rate. The açaí sees were pretreated with 1 % sulfuric acid, 10% solids for 60 minutes at 121 °C. An experimental set 22 for evaluate delignification was performed and the experimental conditions were 1 % NaOH, temperature from 39.64 to 110.36 °C (+a; - a) and solids loading from 3.79 to 46.21 % (-a; +a). After delignification the liquid fraction was characterized for carbohydrate content, lignin and fermentation inhibitors (acetic acid, furfural and HMF). And solid fraction was also characterized for the carbohydrate, lignin and acetic acid. Both fractions were characterized via HPLC. After delignification, the resulting material was enzymatically hydrolyzed with an enzyme loading of 15 FPU/g of seed and 25CBU/g of seeds and 3% of solids and glucose concentrations were measured via HPLC. The results showed that the yield varied from 24 to 49% and delignification was effective in removing up to 89 % of this pre-treated material with dilute sulfuric acid lignin. The condition of 75°C and 3.79 % of solids was the condition that promoted higher enzymatic conversion with a value of 94.76%. It can be concluded that the conditions for delignification studied were effective in removing lignin and improve glucose release during enzymatic hydrolysis4349950412th International Conference on Chemical and Process Engineering (ICheaP)sem informaçã

Challenges and opportunities in lactic acid bioprocess design-From economic to production aspects

Lactic acid is an already consolidated bioproduct in the world market. It has many applications, such as: the production of biodegradable polymers, substitution of plastics from oil, and new uses in medicine. Besides this, new applications are being discovered every year, especially in chemical industries as a building-block molecule. The lactic acid market is in constant growth and the fact that the final products are able to comply with environmental laws as green, renewable and biodegradable products contributes to the tendency of continuous growth in the next few years. With all of this in mind, this paper will explore the main aspects of the sector, as well as market tendencies, production chain, and innovation. (C) 2018 Elsevier B.V. All rights reserved.Foundation for Research of the State of Sao Paulo-FAPESPUniv Campinas Unicamp, Sch Chem Engn, Lab Optimizat Design & Adv Proc Control, 500 Albert Einstein Ave, BR-13083852 Campinas, SP, BrazilFed Univ Sao Paulo UNIFESP, Dept Marine Sci, 144 Carvalho Mendonca St, BR-11070100 Santos, BrazilUniv Campinas Unicamp, Interdisciplinary Ctr Energy Planning NIPE, 330 Cora Coralina St, BR-13083896 Campinas, SP, BrazilFed Univ Sao Paulo UNIFESP, Dept Marine Sci, 144 Carvalho Mendonca St, BR-11070100 Santos, Brazil|FAPESP: 2013/26290-5Web of Scienc