Estimation of methane generation based on anaerobic digestion and mass balance at Kiteezi Landfill, Kampala, Uganda

Kiteezi landfill site is the main solid waste dumping site in Kampala City (Uganda). In this study, the generation of methane from waste at Kiteezi landfill was measured using laboratory-scale anaerobic digestion experiment and estimated using the Mass balance model. The samples were collected in the wet and dry seasons, with five replicates for each season which were processed for further experiments focused on moisture content analysis and anaerobic digestion. The moisture content analysis results showed a significant change (P < 0.05) between wet season and dry season. Also, the anaerobic digestion revealed that moisture content was a determining factor in gas generation. The average monthly methane production estimate from the mass balance model was 1.63 Gg methane/month and was comparable (within 14%) to the amount estimated by laboratory-scale anaerobic digestion experiment (1.43 Gg methane/month). It is a worthwhile undertaking to further investigate the potential of commercially producing methane from Kiteezi landfill as an alternative source of green and clean energy for urban masses.Keywords: Solid waste management, methane generation, anaerobic digestion and mass balance model

Vitamin B12 bioaccumulation in Chlorella vulgaris grown on food waste-derived anaerobic digestate

Anaerobic digestion plays a pivotal role in the modern circular economy, as it offers a sustainable solution for converting organic waste into biogas (methane). It also results in a nutrient-rich liquid stream, referred to as digestate. This digestate is extensively applied to agricultural land as fertilizer due to its high macronutrient (N, P) content, but the bioactive micronutrients it contains and their significance for downstream applications remain largely unknown. Here, we investigate whether digestate generated from a vitamin B12-deficient substrate (fruit and vegetable waste) can be enriched in this vitamin through anaerobic digestion, and explore the capability of the microalga Chlorella vulgaris to grow in this medium and bioaccumulate B12. Our findings uncover, for the first time, that substantial amounts of B12 are synthesized during anaerobic digestion, and that C. vulgaris can effectively be enriched with this vitamin when grown in the digestate (10.6 μg Β12·g−1 dry weight). Additionally, we identified that pH-induced ammonia toxicity was the main inhibitor when growing microalgae in the digestate, which allowed us to significantly enhance productivity at lab- and pilot-scale through pH control. The case of B12 synthesis in digestate and accumulation in microalgae highlights the potential for enhancing the value of these waste streams through the identification and utilization of bioactive compounds