白血球細胞膜抗原に対するモノクローナル抗体を用いた腫瘍シンチグラフィに関する研究

金沢大学医学部研究課題/領域番号:62770777, 研究期間(年度):1987出典：研究課題「白血球細胞膜抗原に対するモノクローナル抗体を用いた腫瘍シンチグラフィに関する研究」課題番号62770777（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） （https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-62770777/）を加工して作

Evaluation of patients who underwent percutaneous transhepatic portal vein embolisation by Tc-99m GSA scintigraphy

Purpose: To analyse the correlation between the fold change in residual liver volume (RLV) and residual liver uptake at 15 (RLU15) before and after percutaneous transhepatic portal vein embolisation (PTPE). Material and methods: Between August 2010 and December 2016, 20 patients who underwent PTPE were retrospectively selected. Before and three weeks after PTPE, contrast-enhanced computed tomography (CECT) and Tc-99m GSA scintigraphy were performed to analyse the fold changes in RLV and RLU15, respectively, as well as their correlation. Results: After PTPE, a significant increase was observed in the RLV (before: 464 ± 99 ml; after: 573 ± 118 ml, p = 0.004) and the RLU15 (before: 11.0 ± 2.9%; after: 17.7 ± 3.8%, p = 5 × 10-7). The fold increase of RLV and RLU15 in all patients was 1.25 ± 0.15 and 1.66 ± 0.33, respectively. No significant correlation was observed in the fold increase in both RLV and RLU15 (r = 0.14, p = 0.66). In patients no. 3 and 9, who were outliers, the increase in RLV was minimal and RLU15 increased greatly, and these 2 patients underwent radical hepatectomy after PTPE. Conclusions: No correlation was observed between the fold increase in RLV and RLU15 before and after PTPE. In order to accurately evaluate the residual liver function, it should be considered necessary to evaluate not only by morphological CECT volumetry, but also by functional outcome of Tc-99m GSA scintigraphy. Residual liver volume may not necessarily reflect RLF. It may be possible to improve the radical resection rate by detecting the potential increase of RLF with RLU15 of Tc-99m GSA scintigraphy

99mTc-DTPAおよび131I-Hippuranによる分腎機能の評価に関する実験的検討-第1報閉塞性尿路障害について-

金沢大学大学院医学系研究

Inhibition of Hsp90 Leads to Cell Cycle Arrest and Apoptosis in Human Malignant Pleural Mesothelioma

IntroductionHeat shock protein 90 (Hsp90) is an abundant molecular chaperone that mediates the maturation and stability of a variety of proteins associated with the promotion of cell growth and survival. Inhibition of Hsp90 function leads to proteasomal degradation of its mis-folded client proteins. Recently, Hsp90 has emerged as being of prime importance to the growth and survival of cancer cells and its inhibitors have already been used in phase I and II clinical trials.MethodsWe investigated how 17-allylamino-17-demethoxygeldanamycin (17-AAG), a small molecule inhibitor of Hsp90, is implicated in human malignant pleural mesothelioma (MM).ResultsWe found that 17-AAG led to significant G1 or G2/M cell cycle arrest, inhibition of cell proliferation, and decrease of AKT, AKT1, and survivin expression in all human malignant pleural mesothelioma cell lines examined. We also observed significant apoptosis induction in all MM cell lines treated with 17-AAG. Furthermore, 17-AAG induced apoptosis in freshly cultured primary MM cells and caused signaling changes identical to those in 17-AAG treated MM cell lines.ConclusionThese results suggest that Hsp90 is strongly associated with the growth and survival of MM and that inhibition of Hsp90 may have therapeutic potential in the treatment of MM

腎シンチグラフィ斜位像の有効性に関する検討-特に限局性病巣検出能に関して-

金沢大学大学院医学系研究

Efficacy of Wnt-1 monoclonal antibody in sarcoma cells

BACKGROUND: Sarcomas are one of the most refractory diseases among malignant tumors. More effective therapies based on an increased understanding of the molecular biology of sarcomas are needed as current forms of therapy remain inadequate. Recently, it has been reported that Wnt-1/β-catenin signaling inhibits apoptosis in several cancers. In this study, we investigated the efficacy of a monoclonal anti-Wnt-1 antibody in sarcoma cells. METHODS: We treated cell lines A-204, SJSA-1, and fresh primary cultures of lung metastasis of sarcoma with a monoclonal anti-Wnt-1 antibody. Wnt-1 siRNA treatment was carried out in A-204. We assessed cell death using Crystal Violet staining. Apoptosis induction was estimated by flow cytometry analysis (Annexin V and PI staining). Cell signaling changes were determined by western blotting analysis. RESULTS: We detected Wnt-1 expression in all tissue samples and cell lines. Significant apoptosis induction was found in monoclonal anti-Wnt-1 antibody treated cells compared to control monoclonal antibody treated cells (p < 0.02). Similarly, we observed increased apoptosis in Wnt-1 siRNA treated cells. Blockade of Wnt-1 signaling in both experiments was confirmed by analyzing intracellular levels of Dishevelled-3 and of cytosolic β-catenin. Furthermore, the monoclonal anti-Wnt-1 antibody also induced cell death in fresh primary cultures of metastatic sarcoma in which Wnt-1 signaling was active. CONCLUSION: Our results indicate that Wnt-1 blockade by either monoclonal antibody or siRNA induces cell death in sarcoma cells. These data suggest that Wnt-1 may be a novel therapeutic target for the treatment of a subset of sarcoma cells in which Wnt-1/β-catenin signaling is active

ダイガクセイ ノ スポーツ カツドウ ニ カンスル カチイシキ ノ ケントウ : ナツヤスミ ノ スポーツ カツドウ トノ カンレン カラ

本研究は、第一に大学生がスポーツ活動に関してどの程度の価値意識を有しているものなのかを明らかにすることである。そして第二に大学生が価値を有しているであろうと思われるスポーツ活動について生活行動が最も多様になる時期、言い換えれば自由な時間が最も取れる夏休みにどの程度スポーツ活動を行っているのかその実態を明らかにすることを試みた。また、大学体育におけるスポーツの生活化指導のための基礎的な資料を得ることも目的の一つである。結果、大学生はスポーツの持っている価値は認識していたが、夏休み中にスポーツ活動を行った大学生は、半数以下であった。夏休みは多くの自由時間があるものの、運動やスポーツをするといった体を動かすことをするよりも、大学生は、「何もしない」とか「テレビやビデオ視聴」で過ごすということに時間を使っていた。このような大学生の時間の使い方についてもう少し詳細に調べてみなければはっきりとしたことは言えないが、深夜までテレビやビデオ(DVD)の視聴をしていて、日中は休んでいるという生活リズムになってしまっているのではと推察する。大学生は、自由な時間がある、なしにかかわらず、スポーツは価値のある行為だとはわかっていてもスポーツをすること自体に意識が向いていないから以上のような結果になると考えられる。It is necessary to acquire a manner, the ability that can manage the time when a university student is free by oneself premeditatedly. I think that the ability becomes the base of sports throughout the life.This study examined value awareness about the sports activity of the university studentand how to spend free time of the summer vacation of the university student and the connection of the exercise custom.1. The university student recognizes the value that sports have.2. The university students who worked on sports during summer vacation were lessthan half. 3. Spend it by "TV and the video seeing and hearing" that the university student "does not do as for what than much free time moves exercise and a body to play sports in the summer vacation either"; do it.4. The university student who did not work on sports tended not to work part-time.5. When sports are valuable acts, it is understood, but it is thought that the consciousness of the university student is not suitable for playing sports.6. The teacher must provide a lecture letting you acquire a manner, the ability that canmanage the sports activity in a class by oneself premeditatedly using the time when auniversity student is free