Uso do microscópio de luz polarizada para análise de tendinítes de equinos tratadas com polisulfato de glicosaminoglicano

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Comparative study using 685-nm and 830-nm lasers in the tissue repair of tenotomized tendons in the mouse

Objective: The objective of this study was to evaluate the effects of 685- and 830-nm laser irradiations, at different fluences on the healing process of Achilles tendon (Tendon calcaneo) of mice after tenotomy. Background Data: Some authors have shown that low-level laser therapy (LLLT) is able to accelerate the healing process of tendinuos tissue after an injury, increasing fibroblast cell proliferation and collagen synthesis. However, the mechanism by which LLLT acts on healing process is not fully understood. Methods: Forty-eight male mice were divided into six experimental groups: group A, tenomized animals, treated with 685 nm laser, at the dosage of 3 J/cm(2); group B, tenomized animals, treated with 685-nm laser, at the dosage of 10 J/cm(2); group C, tenomized animals, treated with 830-nm laser, at dosage of 3 J/cm(2); group D, tenomized animals, treated with 830-nm laser, at the dosage of 10 J/cm(2); group E, injured control (placebo treatment); and group F, non-injured standard control. Animals were killed on day 13 post-tenotomy, and their tendons were surgically removed for a quantitative analysis using polarization microscopy, with the purpose of measuring collagen fibers organization trough the birefringence (optical retardation [OR]). Results: All treated groups showed higher values of OR when compared to injured control group. The best organization and aggregation of the collagen bundles were shown by the animals of group A (685 run, 3 J/cm(2)), followed by the animals of group C and B, and finally, the animals of group D. Conclusion: All wavelengths and fluences used in this study were efficient at accelerating the healing process of Achilles tendon post-tenotomy, particularly after the 685-nm laser irradiation, at 3 J/cm(2). It suggests the existence of wavelength tissue specificity and dose dependency. Further studies are required to investigate the physiological mechanisms responsible for the effects of laser on tendinuos repair.o TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE AGOSTO DE 2015.24675475

Comparação do ultrassom pulsado e contínuo no reparo tendíneo de ratos Comparison of pulsed and continuous ultrasound on tendon repair in rats

No tratamento de lesões tendíneas, o uso do ultrassom surge como possibilidade terapêutica, apesar de lacunas sobre seus efeitos clínicos. O objetivo foi avaliar dois protocolos de ultrassom terapêutico sobre dor e edema após trauma tendíneo. Vinte e um ratos Wistar foram submetidos a trauma no tendão calcâneo e divididos em três grupos: sham (GS); ultrassom contínuo (GUC); e ultrassom pulsado (GUP). O trauma ocorreu sobre a face lateral do tendão calcâneo direito, com energia de 0,40 J. A dor foi avaliada pelo teste de incapacidade funcional e o edema, pelo diâmetro laterolateral. Foram realizadas avaliações previamente à lesão; após 1 hora da indução da lesão; após o 1º tratamento; 2, 8 e 24 horas após lesão; e após o 5º dia. O tratamento ocorreu em 5 dias, com transdutor de 1 MHz, durante 3 minutos, sobre o local do trauma, com dose de 0,4 W/cm² SATA. Os resultados da incapacidade funcional para GS mostraram aumento da nocicepção. Para GUC houve aumento ao comparar a avaliação 1 (AV1) com as avaliações 2 (AV2), 3 (AV3) e 4 (AV4); ao comparar AV2 com as avaliações 5 (AV5) e 6 (AV6) houve diminuição de valores. Para GUP houve aumento ao comparar AV1 com AV2 e AV3, mas ao comparar AV2 com as seguintes, houve diminuição significativa a partir de AV4. Para o edema, os grupos tratados produziram aumento inicial, com redução nas últimas avaliações. O ultrassom terapêutico produziu diminuição de dor e edema, mais precocemente para a forma pulsada.<br>In tendon injuries treatment, the use of ultrasound appears as a therapeutic option, despite the lack of their clinical effects. The aim was to evaluate two therapeutic ultrasound protocols, continuous or pulsed, on the pain and swelling after tendinous trauma. Twenty-one Wistar rats were subjected to traumatic injury in the tendon and divided in three groups: sham (GS), continuous ultrasound (GUC), and pulsed ultrasound (GUP). The injury occurred on right Achilles tendon lateral aspect, with energy of 0.40 J. The pain was assessed by the functional disability test and edema by latero-lateral diameter. The evaluations were performed before the injury; after 1 hour of the lesion induction; after the 1st treatment; 2, 8 and 24 hours after injury; and after the 5th day. The treatment was made for 5 consecutive days, with transductor of 1 MHz, for 3 minutes on the trauma site, with a dose of 0.4 W/cm² SATA. The results of functional disability for GS showed increased nociception. For GUC, there was increased when comparing evaluation 1 (EV1) with evaluations 2 (EV2), 3 (EV3) and 4 (EV4); comparing EV2 to evaluations 5 (AV5) and 6 (AV6), there was decreased values. To GUP there was increased when comparing EV1 with EV2 and EV3, but when comparing EV2 with the following, there was a significant decrease starting from EV4. Edema, treatment groups produced an initial increase with a reduction in recent evaluations. The therapeutic ultrasound produced pain and edema reduction in rats with tendon trauma, and early in the pulsed