Influence of Cooling Flow and Galactic Motion on the Iron Distribution in Clusters of Galaxies

Iron abundance distribution is now known for 12 clusters of galaxies. For some clusters (e.g. Centaurus) the observed abundance increases toward the cluster center, while for the others (e.g. Coma and Hydra-A) no significant inhomogeneity was observed. In order to understand this difference, we investigate the influence of cooling flow and turbulence produced by galactic motion on the iron abundance distribution by simple spherical models. We show that the cooling flow has a significant effect to flatten the iron abundance distribution if the flow velocity is sufficiently large. Further, by applying our analysis to the above clusters we show that we can give a systematic account for the observed variety of the iron abundance distribution qualitatively.Comment: 30 pages, uuencoded compressed postscript with figures, YITP/U-94-2

Effects of Ram-Pressure from Intracluster Medium on the Star Formation Rate of Disk Galaxies in Clusters of Galaxies

Using a simple model of molecular cloud evolution, we have quantitatively estimated the change of star formation rate (SFR) of a disk galaxy falling radially into the potential well of a cluster of galaxies. The SFR is affected by the ram-pressure from the intracluster medium (ICM). As the galaxy approaches the cluster center, the SFR increases to twice the initial value, at most, in a cluster with high gas density and deep potential well, or with a central pressure of $\sim 10^{-2} cm^{-3} keV$ because the ram-pressure compresses the molecular gas of the galaxy. However, this increase does not affect the color of the galaxy significantly. Further into the central region of the cluster ($\lesssim 1$ Mpc from the center), the SFR of the disk component drops rapidly due to the effect of ram-pressure stripping. This makes the color of the galaxy redder and makes the disk dark. These effects may explain the observed color, morphology distribution and evolution of galaxies in high-redshift clusters. By contrast, in a cluster with low gas density and shallow potential well, or the central pressure of $\sim 10^{-3} cm^{-3} keV$, the SFR of a radially infalling galaxy changes less significantly, because neither ram-pressure compression nor stripping is effective. Therefore, the color of galaxies in poor clusters is as blue as that of field galaxies, if other environmental effects such as galaxy-galaxy interaction are not effective. The predictions of the model are compared with observations.Comment: 19 pages, 9 figures, to appear in Ap

Analysis of Flexural Behavior and Lateral Buckling of Inelastic Steel Beams under Cyclic Loads

Inelastic steel beams are analyzed with emphasis on their transient flexural behavior and lateral buckling under cyclic loads. The constraint and load conditions are chosen so that they simulate inelastic beams, of a frame structure subjected to a horizontal seismic motion. An analytical model of inelastic beams is proposed that accounts for basic transient behaviors of mild steel. On this basis, a detailed discussion is made on the mechanism of transient behaviors including those of the plasic hinge, loaddeflection relation, lateral buckling load, etc. A physical interpretation is given as regards the transient flexural behavior and the deformation capacity for the lateral buckling of steel beams under monotonic and cyclic loadings

Lipid Membrane Interaction of Peptide/DNA Complexes Designed for Gene Delivery

Among gene delivery systems, peptide-based gene carriers have received significant attention because of their selectivity, biocompatibility, and biodegradability. Since cellular membranes function as a barrier toward exogenous molecules, cell-penetrating peptides (CPPs), which are usually cationic and/or amphiphilic, can serve as efficient carriers to deliver cargo into the cytosol. Here, we examined the interactions of carrier peptides and their DNA complexes with lipid membranes using a quartz crystal microbalance (QCM) and high-speed atomic force microscopy (HS-AFM). The carrier peptides are a 12-residue partial presequence of yeast cytochrome c oxidase subunit IV (Cytcox) and BP100, which are a mitochondria-targeting signal peptide and a CPP, respectively. QCM data showed that BP100 has a higher binding affinity than Cytcox to both plasma membrane- and mitochondrial membrane-mimicking lipid bilayers. The DNA complexes with either Cytcox or BP100 exhibited the same tendency. Furthermore, HS-AFM data demonstrated that the DNA complexes of either peptide can disrupt the lipid membranes, forming larger pores in the case of Cytcox. Our results suggest that the binding affinity of the peptide/DNA complex to the plasma membrane is more critical than its membrane disruption ability in enhancing the cellular uptake of DNA

Improved G-AgarTrap: A highly efficient transformation method for intact gemmalings of the liverwort Marchantia polymorpha

Liverworts are key species for studies of plant evolution, occupying a basal position among the land plants. Marchantia polymorpha has emerged as a highly studied model liverwort, and many relevant techniques, including genetic transformation, have been established for this species. Agrobacterium-mediated transformation is widely used in many plant species because of its low cost. Recently, we developed a simplified Agrobacterium-mediated method for transforming M. polymorpha, known as AgarTrap (agar-utilized transformation with pouring solutions). The AgarTrap procedure, which involves culturing the liverwort tissue in various solutions on a single solid medium, yields up to a hundred independent transformants. AgarTrap is a simple procedure, requiring minimal expertise, cost, and time. Here, we investigated four factors that influence AgarTrap transformation efficiency: (1) humidity, (2) surfactant in the transformation buffer, (3) Agrobacterium strain, and (4) light/dark condition. We adapted the AgarTrap protocol for transforming intact gemmalings, achieving an exceptionally high transformation efficiency of 97%. The improved AgarTrap method will enhance the molecular biological study of M. polymorpha. Furthermore, this method provides new possibilities for improving transformation techniques for a variety of plant species

Purely excitonic lasing in ZnO microcrystals: Temperature-induced transition between exciton-exciton and exciton-electron scattering

Since the seminal observation of room-temperature laser emission from ZnO thin films and nanowires, numerous attempts have been carried out for detailed understanding of the lasing mechanism in ZnO. In spite of the extensive efforts performed over the last decades, the origin of optical gain at room temperature is still a matter of considerable discussion. In this work, we show that a ZnO film consisting of well-packed micrometer-sized ZnO crystals exhibits purely excitonic lasing at room temperature without showing any symptoms of electron-hole plasma emission, even under optical excitation more than 25 times above the excitonic lasing threshold. The lasing mechanism is shifted from the exciton-exciton scattering to the exciton-electron scattering with increasing temperature from 3 to 150 K. The exciton-electron scattering process continues to exist with further increasing temperature from 150 to 300 K. Thus, we present distinct experimental evidence that the room-temperature excitonic lasing is achieved not by exciton-exciton scattering, as has been generally believed, but by exciton-electron scattering. We also argue that the long carrier diffusion length and the low optical loss nature of the micrometer-sized ZnO crystals, as compared to those of ZnO nanostructures, plays a key role in showing room-temperature excitonic lasing

Bilirubin is produced nonenzymatically in plants to maintain chloroplast redox status

血液の分解産物ビリルビンが植物で作られることを発見 --植物の効率的な光合成に寄与している可能性--. 京都大学プレスリリース. 2023-06-08.Bilirubin, a potent antioxidant, is a product of heme catabolism in heterotrophs. Heterotrophs mitigate oxidative stress resulting from free heme by catabolism into bilirubin via biliverdin. Although plants also convert heme to biliverdin, they are generally thought to be incapable of producing bilirubin because they lack biliverdin reductase, the enzyme responsible for bilirubin biosynthesis in heterotrophs. Here, we demonstrate that bilirubin is produced in plant chloroplasts. Live-cell imaging using the bilirubin-dependent fluorescent protein UnaG revealed that bilirubin accumulated in chloroplasts. In vitro, bilirubin was produced nonenzymatically through a reaction between biliverdin and reduced form of nicotinamide adenine dinucleotide phosphate at concentrations comparable to those in chloroplasts. In addition, increased bilirubin production led to lower reactive oxygen species levels in chloroplasts. Our data refute the generally accepted pathway of heme degradation in plants and suggest that bilirubin contributes to the maintenance of redox status in chloroplasts

Organellar Glue: A Molecular Tool to Artificially Control Chloroplast–Chloroplast Interactions

細胞小器官を接着する新技術「オルガネラグルー」を開発 --オルガネラ間コミュニケーションの操作に期待--. 京都大学プレスリリース. 2022-09-30.Organelles can physically interact to facilitate various cellular processes such as metabolite exchange. Artificially regulating these interactions represents a promising approach for synthetic biology. Here, we artificially controlled chloroplast–chloroplast interactions in living plant cells with our organelle glue (ORGL) technique, which is based on reconstitution of a split fluorescent protein. We simultaneously targeted N-terminal and C-terminal fragments of a fluorescent protein to the chloroplast outer envelope membrane or cytosol, respectively, which induced chloroplast–chloroplast interactions. The cytosolic C-terminal fragment likely functions as a bridge between two N-terminal fragments, thereby bringing the chloroplasts in close proximity to interact. We modulated the frequency of chloroplast–chloroplast interactions by altering the ratio of N- and C-terminal fragments. We conclude that the ORGL technique can successfully control chloroplast–chloroplast interactions in plants, providing a proof of concept for the artificial regulation of organelle interactions in living cells

Deuterium- and Alkyne-Based Bioorthogonal Raman Probes for In Situ Quantitative Metabolic Imaging of Lipids within Plants

Plants can rapidly respond to different stresses by activating multiple signaling and defense pathways. The ability to directly visualize and quantify these pathways in real time using bioorthogonal probes would have practical applications, including characterizing plant responses to both abiotic and biotic stress. Fluorescence-based labels are widely used for tagging of small biomolecules but are relatively bulky and with potential effects on their endogenous localization and metabolism. This work describes the use of deuterium- and alkyne-derived fatty acid Raman probes to visualize and track the real-time response of plants to abiotic stress within the roots. Relative quantification of the respective signals could be used to track their localization and overall real-time responses in their fatty acid pools due to drought and heat stress without labor-intensive isolation procedures. Their overall usability and low toxicity suggest that Raman probes have great untapped potential in the field of plant bioengineering