Zonation of Central U. S. Earthquake Sources

A variety of analyses are utilized in developing potential earthquake ground shaking at a specific location. Geological procedures for estimating causes of earthquakes are fundamental to the prediction of ground motions. Evaluations of geologic factors compliment mathematical assessments of seismological data. Earthquake potential in the Central United States is predicted using the concept of seismic source zones due to the difficulty of determining active faults. Resolution of these geographic source zones is dependent upon knowledge of historic seismicity, pertinent geologic features, and causative tectonics. Regardless of the method used to delineate source zones, these zones must be geologically and seismologically unique. Statistical testing of historic earthquake catalogues is required for reduction of the data base. The resolution of zones is an iterative process of bounding the zones and determining recurrence rates. Earthquake potential and risk assessment should be understood by the owner and the designer of a facility

Beef cows and calves, 1979: a summary of research

Response of fall-born calves to monensin on orchardgrass / alfalfa or tall fescue / alfalfa pastures / F. M. Byers, C. F. Parker, and R. W. Van Keuren -- Effects of forage system and breed type on the performance of fall calving cows / C. F. Parker and R. W. Van Keuren -- Forage management for beef production / R. W. Van Keuren, C. F. Parker, and E. W. Klosterman -- Breeding and management systems to optimize beef breeding herd productivity / E. W. Klosterman, R. W. Van Keuren, C. F. Parker, and F. M. Byers -- Voluntary feed intake of mature cows as related to breed type, condition, and forage quality / E. W. Klosterman, F. M. Byers, and C. F. Parker -- Weight and condition changes of pregnant beef cows wintered on corn stover stacks / G. R. Wilson, J. G. Gordon, J. H. Cline, K. M. Irvin, and E. W. Klosterman -- Estrus synchronization of beef cows and heifers with prostaglandin F2a under field conditions / G. R. Wilson, T. L. Benecke, K. M. Irvin, T. M. Ludwick, C. E. Marshall, and R. A. Wallac

Deriving the number of jobs in proximity services from the number of inhabitants in French rural municipalities

We use a minimum requirement approach to derive the number of jobs in proximity services per inhabitant in French rural municipalities. We first classify the municipalities according to their time distance to the municipality where the inhabitants go the most frequently to get services (called MFM). For each set corresponding to a range of time distance to MFM, we perform a quantile regression estimating the minimum number of service jobs per inhabitant, that we interpret as an estimation of the number of proximity jobs per inhabitant. We observe that the minimum number of service jobs per inhabitant is smaller in small municipalities. Moreover, for municipalities of similar sizes, when the distance to the MFM increases, we find that the number of jobs of proximity services per inhabitant increases.Comment: 6 pages, 5 figure

A single-nucleotide mutation in GLUTAMATE RECEPTOR-LIKE protein gene confers resistance to Fusarium wilt in Gossypium hirsutum

Fusarium wilt (FW) disease of cotton, caused by the fungus Fusarium oxysporum f. sp. vasinfectum (Fov), causes severe losses in cotton production worldwide. Though significant advancements have been made in development of FW‐resistant Upland cotton (Gossypium hirsutum) in resistance screening programs, the precise resistance genes and the corresponding molecular mechanisms for resistance to Fov remain unclear. Herein it is reported that Fov7, a gene unlike canonical plant disease‐resistance (R) genes, putatively encoding a GLUTAMATE RECEPTOR‐LIKE (GLR) protein, confers resistance to Fov race 7 in Upland cotton. A single nucleotide polymorphism (SNP) (C/A) in GhGLR4.8, resulting in an amino acid change (L/I), is associated with Fov resistance. A PCR‐based DNA marker (GhGLR4.8SNP(A/C)) is developed and shown to cosegregate with the Fov resistance. CRISPR/Cas9‐mediated knockout of Fov7 results in cotton lines extremely susceptible to Fov race 7 with a loss of the ability to induce calcium influx in response to total secreted proteins (SEPs) of Fov. Furthermore, coinfiltration of SEPs with GhGLR4.8A results in a hypersensitive response. This first report of a GLR‐encoding gene that functions as an R gene provides a new insight into plant–pathogen interactions and a new handle to develop cotton cultivars with resistance to Fov race 7

Identification of Pathogenicity-Related Genes in the Vascular Wilt Fungus Verticillium dahliae by Agrobacterium tumefaciens-Mediated T-DNA Insertional Mutagenesis

Verticillium dahliae is the causal agent of vascular wilt in many economically important crops worldwide. Identification of genes that control pathogenicity or virulence may suggest targets for alternative control methods for this fungus. In this study, Agrobacteriumtumefaciens-mediated transformation (ATMT) was applied for insertional mutagenesis of V. dahliae conidia. Southern blot analysis indicated that T-DNAs were inserted randomly into the V. dahliae genome and that 69% of the transformants were the result of single copy T-DNA insertion. DNA sequences flanking T-DNA insertion were isolated through inverse PCR (iPCR), and these sequences were aligned to the genome sequence to identify the genomic position of insertion. V. dahliae mutants of particular interest selected based on culture phenotypes included those that had lost the ability to form microsclerotia and subsequently used for virulence assay. Based on the virulence assay of 181 transformants, we identified several mutant strains of V. dahliae that did not cause symptoms on lettuce plants. Among these mutants, T-DNA was inserted in genes encoding an endoglucanase 1 (VdEg-1), a hydroxyl-methyl glutaryl-CoA synthase (VdHMGS), a major facilitator superfamily 1 (VdMFS1), and a glycosylphosphatidylinositol (GPI) mannosyltransferase 3 (VdGPIM3). These results suggest that ATMT can effectively be used to identify genes associated with pathogenicity and other functions in V. dahliae