76 research outputs found
Evaluation of Short-term Myelotoxicity Study in Dietary Reduced Rats
This study attempted to prove our hypothesis that a short-term toxicity study,
using a 4-day dosing regimen as an example, is suitable for evaluating
myelotoxicity in rats. We compared the hematological, bone marrow cytological
and histopathological results of 5-fluorouracil (5-FU) treated and pair-feeding
groups after a 4-day administration period. Several experimental groups were
defined for this 4-day study as well as for our previously reported 14-day study
(Miyata et al., 2009); these included 5-FU treated groups
receiving 12, 15 and 18 mg/kg/day (FU12, FU15 and FU18), pair-feeding groups
(R12, R15 and R18 receiving the same amount of food as the FU12, FU15 and FU18
groups, respectively) and a nontreated control group. Although severe reductions
in body weight gain and food consumption were reported in the 14-day study, only
slight reductions were observed in the 4-day study. In the 4-day study, a
decrease in blood reticulocytes and a decreasing trend of marrow erythroid cells
were only observed in the FU18 group, and no effects were observed in the
pair-feeding groups. The erythroblastic changes observed in this 4-day study
were thought to reflect the direct influence of 5-FU administration. Since
concerns regarding the influence of secondary changes related to undernutrition
were minimized in the 4-day study, it was thought to clarify the direct
influence of 5-FU administration on erythroblastic cells. Thus, a 4-day study
protocol might be helpful for distinguishing secondary changes related to
undernutrition
Identification of myogenic-endothelial progenitor cells in the interstitial spaces of skeletal muscle
Putative myogenic and endothelial (myo-endothelial) cell progenitors were identified in the interstitial spaces of murine skeletal muscle by immunohistochemistry and immunoelectron microscopy using CD34 antigen. Enzymatically isolated cells were characterized by fluorescence-activated cell sorting on the basis of cell surface antigen expression, and were sorted as a CD34+ and CD45− fraction. Cells in this fraction were ∼94% positive for Sca-1, and mostly negative (<3% positive) for CD14, 31, 49, 144, c-kit, and FLK-1. The CD34+/45− cells formed colonies in clonal cell cultures and colony-forming units displayed the potential to differentiate into adipocytes, endothelial, and myogenic cells. The CD34+/45− cells fully differentiated into vascular endothelial cells and skeletal muscle fibers in vivo after transplantation. Immediately after sorting, CD34+/45− cells expressed only c-met mRNA, and did not express any other myogenic cell-related markers such as MyoD, myf-5, myf-6, myogenin, M-cadherin, Pax-3, and Pax-7. However, after 3 d of culture, these cells expressed mRNA for all myogenic markers. CD34+/45− cells were distinct from satellite cells, as they expressed Bcrp1/ABCG2 gene mRNA (Zhou et al., 2001). These findings suggest that myo-endothelial progenitors reside in the interstitial spaces of mammalian skeletal muscles, and that they can potentially contribute to postnatal skeletal muscle growth
Belediye Müzesi
Taha Toros Arşivi, Dosya No: 114-Müzelerİstanbul Kalkınma Ajansı (TR10/14/YEN/0033) İstanbul Development Agency (TR10/14/YEN/0033
Blood Flow Simulation System with Interaction between Blood Flow and Blood Vessel Wall using Image Based Cartesian Grid
For the simulation of the fluid-structure interaction (FSI) between the blood flow and blood vessel walls, we have examined the voxel-based FSI method. This method uses a Cartesian grid, called voxel, made from medical images. Further, we have tested the accuracy and reliability of this simple method and have observed its features. In this document, we discuss the background, kinetic models of the blood vessel, a numerical method, and the result of an experiment conducted using an artificial identical shape and an actual realistic shape
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