Arts in Health in the Netherlands:A national agenda

Arts in health is the field that advances the use of artistic practices for health and well-being, to stimulate a positive approach to health. While arts in health fields have been successfully established in other countries, the Dutch field is still fragmented, and its potential has not been fully developed.This white paper is an intersectoral effort exploring the current state of arts in health in the Netherlands. The authors strongly believe that arts in health can help navigate challenges faced by our healthcare system in the coming decades, and we have formulated an agenda to establish a sustainable Dutch arts in health field.Arts in health is the field that advances the use of artistic practices for health and well-being, to stimulate a positive approach to health. While arts in health fields have been successfully established in other countries, the Dutch field is still fragmented, and its potential has not been fully developed.This white paper is an intersectoral effort exploring the current state of arts in health in the Netherlands. The authors strongly believe that arts in health can help navigate challenges faced by our healthcare system in the coming decades, and we have formulated an agenda to establish a sustainable Dutch arts in health field

Galectin 3 regulates HCC cell invasion by RhoA and MLCK activation.

Hepatocellular carcinoma (HCC) carries a poor prognosis with no effective treatment available other than liver transplantation for selected patients. Vascular invasion of HCC is one of the most important negative predictor of survival. As the regulation of invasion of HCC cells is not well understood, our aim was to study the mechanisms by which galectin 3, a β-galactosidase-binding lectin mediates HCC cell migration. HCC was induced by N-diethylnitrosamine in wild-type and galectin 3(-/-) mice, and tumor formation, histology, and tumor cell invasion were assessed. The galectin 3(-/-) mice developed significantly smaller tumor burden with a less invasive phenotype than the wild-type animals. Galectin 3 was upregulated in the wild-type HCC tumor tissue, but not in the surrounding parenchyma. Galectin 3 expression in HCC was induced by NF-κB transactivation as determined by chromatin immunoprecipitation assays. In vitro studies assessed the pro-migratory effects of galectin 3. The migration of hepatoma cells was significantly decreased after transfection by the galectin 3 siRNA and also after using the Rho kinase inhibitor Y-27632. The reorganization of the actin cytoskeleton, RhoA GTPase activity and the phosphorylation of MLC2 (myosin light chain 2) were decreased in the galectin 3 siRNA-transfected cells. In addition, in vitro and in vivo evidence showed that galectin 3 deficiency reduced hepatoma cell proliferation and increased their apoptosis rate. In conclusion, galectin 3 is an important lectin that is induced in HCC cells, and promotes hepatoma cell motility and invasion by an autocrine pathway. Targeting galectin 3 therefore could be an important novel treatment strategy to halt disease progression

Arts in Health in the Netherlands:A national agenda

Arts in health verwijst naar het veld dat artistieke en creatieve praktijken toepast om gezondheid en welzijn te bevorderen en een positieve benadering van gezondheid te stimuleren. Terwijl arts in health internationaal al decennialang een gevestigd veld is in andere landen, is het Nederlandse veld nog steeds gefragmenteerd en blijft het groeiende potentieel ervan onderbenut. Deze whitepaper is een intersectorale verkenning van de huidige stand van zaken op het gebied van arts in health in Nederland. De auteurs zijn ervan overtuigd dat arts in health kan helpen om de grote uitdagingen waar onze gezondheidszorg de komende decennia voor staat, het hoofd te bieden en hebben een agenda geformuleerd voor het verduurzamen van het veld voor arts in health in Nederland.Arts in health is the field that advances the use of artistic practices for health and well-being, to stimulate a positive approach to health. While arts in health fields have been successfully established in other countries, the Dutch field is still fragmented, and its potential has not been fully developed.This white paper is an intersectoral effort exploring the current state of arts in health in the Netherlands. The authors strongly believe that arts in health can help navigate challenges faced by our healthcare system in the coming decades, and we have formulated an agenda to establish a sustainable Dutch arts in health field

Arts in Health in the Netherlands:A national agenda

Arts in health verwijst naar het veld dat artistieke en creatieve praktijken toepast om gezondheid en welzijn te bevorderen en een positieve benadering van gezondheid te stimuleren. Terwijl arts in health internationaal al decennialang een gevestigd veld is in andere landen, is het Nederlandse veld nog steeds gefragmenteerd en blijft het groeiende potentieel ervan onderbenut. Deze whitepaper is een intersectorale verkenning van de huidige stand van zaken op het gebied van arts in health in Nederland. De auteurs zijn ervan overtuigd dat arts in health kan helpen om de grote uitdagingen waar onze gezondheidszorg de komende decennia voor staat, het hoofd te bieden en hebben een agenda geformuleerd voor het verduurzamen van het veld voor arts in health in Nederland.Arts in health is the field that advances the use of artistic practices for health and well-being, to stimulate a positive approach to health. While arts in health fields have been successfully established in other countries, the Dutch field is still fragmented, and its potential has not been fully developed.This white paper is an intersectoral effort exploring the current state of arts in health in the Netherlands. The authors strongly believe that arts in health can help navigate challenges faced by our healthcare system in the coming decades, and we have formulated an agenda to establish a sustainable Dutch arts in health field

Galectin 3 regulates HCC cell invasion by RhoA and MLCK activation

Hepatocellular carcinoma (HCC) carries a poor prognosis with no effective treatment available other than liver transplantation for selected patients. Vascular invasion of HCC is one of the most important negative predictor of survival. As the regulation of invasion of HCC cells is not well understood, our aim was to study the mechanisms by which galectin 3, a β-galactosidase binding lectin mediates HCC cell migration. HCC was induced by N-diethylnitrosamine (DEN) in wild type and galectin 3(−/−) mice, and tumor formation, histology, and tumor cell invasion were assessed. The galectin 3(−/−) mice developed significantly smaller tumor burden with a less invasive phenotype than the wild type animals. Galectin 3 was upregulated in the wild type HCC tumor tissue, but not in the surrounding parenchyma. Galectin 3 expression in HCC was induced by NF-κB transactivation as determined by chromatin immunoprecipitation assays. In vitro studies assessed the pro-migratory effects of galectin 3. The migration of hepatoma cells was significantly decreased after transfection by the galectin 3 siRNA and also after using the Rho kinase (ROCK) inhibitor Y-27632. The reorganization of the actin cytoskeleton, RhoA GTP ase activity and the phosphorylation of MLC2 were decreased in the galectin 3 siRNA-transfected cells. In addition, in vitro and in vivo evidence showed that galectin 3 deficiency reduced hepatoma cell proliferation and increased their apoptosis rate. In conclusion, galectin 3 is an important lectin that is induced in HCC cells, and promotes hepatoma cell motility and invasion by an autocrine pathway. Targeting galectin 3 therefore could be an important novel treatment strategy to halt disease progression