Assessment of Oral Health Care Delivery System in Greater Noida Using Five A’s Model

BACKGROUND: Access to dental health services refers not only to utilization but also to the extent by which the utilization is judged as per the professional norms using five independent dimensions of accessibility, availability, accommodation, affordability and acceptability. AIM: The aim of the study is to assess the dental services utilization among population of Greater Noida using Five A’s model. MATERIALS AND METHOD: The study was conducted in Dental College in Greater Noida. This cross-sectional study was carried out on the 200 subjects using convenient sampling on the patients visiting dental OPD.A self-administered structured questionnaire in English and Hindi language was used. Data was entered in the Microsoft excel sheet and analysed using SPSS (version 20.0).RESULTS: Mean level of access to dental services in the study population was 60.3.Corresponding figures for affordability, availability, accessibility, accommodation and acceptability were 55.2 ± 12.1,57.1± 12.8,60.75 ± 14.7,61.75 ± 8.7,58.65± 11.4 respectively.CONCLUSION: According to the results of our study , the level of access to dental care services is not very good with family income, location and level of education being the determinants of this access

Self-reported oral health and denture satisfaction in partially and completely edentulous patients

Context: Edentulism is a handicap that affects the quality of life and nutrition. Tooth loss leads to functional impairment at the level of the orofacial system, significantly interfering with chewing, swallowing and speech, as well as to temporomandibular joint dysfunction, disorders of intermaxillary relation, and facial physiognomy. Aim: The aim of the study was to explore variables that influence self-reported oral health and denture satisfaction in partially and completely edentulous patients using oral health impact profile for edentulous (OHIP-EDENT). Materials and Methods: A hospital-based study was carried out on the patient's age ranged from 30 to 70 years in the dental college who had been treated with complete dentures and removable partial denture using convenience sampling method. Prevalidated questionnaire consisting of OHIP-EDENT was self-administered to all the study participants. The information about the appetite, perceived general and oral health, and satisfaction from the dentures was recorded using a 4-point Likert scale. Data were collected, entered, and analyzed using SPSS version 20.0. One-way ANOVA was performed to test for comparison between different denture groups. P < 0.05 shows statistically significant difference. Results: When the mean of denture satisfaction level was calculated between different denture group and different domains of OHIP-EDENT, there was no difference found in level of denture satisfaction between different denture groups, i.e., overall denture satisfaction mean in Group 1 was 2.18 ± 0.32, Group 2 has 2.23 ± 0.36, Group 3 has the mean of 2.18 ± 0.42, Group 4 has 2.23 ± 0.67, and P value was 0.810 which shows nonsignificant difference. Conclusion: The results of the present study suggest that the poor self-perceived satisfaction among denture wearers affect their quality of life

Genitourinary tuberculosis: clinical profile, diagnostic approach and treatment outcome in a tertiary care center of North India

Introduction:Genitourinary tuberculosis (GUTB) is the second commonest form of extrapulmonary tuberculosis with more than 90% of cases occurring in developing countries and kidney being the most common site of involvement. We present clinico-epidemiological profile of patients and management of genitourinary tuberculosis in IGMC Shimla.Material and methods: We conducted cross sectional record-based study of patients diagnosed and treated for genitourinary tuberculosis in the Department of Urology, IGMC Shimla from January 2017 to November 2020.Results: Eighty-six patients were treated for GUTB and mean age of patients was 42.2 years (18-78 years). In clinical presentation, irritative voiding symptoms i.e., frequency and dysuria (80.23% and 46.51% respectively) were the most common, followed by flank pain and weight loss (40.69% each), further followed by low grade fever (34.88%) and hematuria (33.72%). All patients were started on ATT from DOTS center and then were transferred to local DOTS units of their respective districts. Three patients required modification of ATT due to significant side effects. One patient died as a result of complications related to ATT and comorbid illness.Ultrasound guided percutaneous nephrostomy (PCN), Double J stenting and pigtail drainage for psoas abscess was done in 24 (27.91%) patients, 22 (25.58%) patients and in 8 (9.3%) patients respectively. Reconstructive surgery was done in 25 patients i.e., ureteroureterostomy in 3 (3.48%) patients, ureteric reimplant in 14 patients (16.27%) patients ,augmentation ileocystoplasty in 4 (4.65%) patients, Boari flap in 4 (4.65%) patients. Nephrectomy was done in 3 (3.48%) patients and cystectomy with ileal conduit was done in 3 (3.48%) patients. Conclusion: GUTB is a prevalent disease in our country although data regarding GUTB is quite limited. GUTB complaints are mostly trivial and nonspecific but consequences are grave. Proper early diagnosis and timely management can prevent morbidity and potential mortality in these patients. Timely intervention and reconstructive surgery is required in a significant number of patients to preserve kidney, ureter, and urinary bladder function

Effect of Storage of Fruit on Quality of Citrus Peel Essential Oil

Physical and chemical quality of essential oil extracted from peel of four stored citrus species (Jatti khatti, Soh sarkar, grapefruit and pummelo) were determined. Refractive indices of peel oil from Jatti khatti, Soh sarkar, grapefruit and pummelo were 1.467, 1.469, 1.471 and 1.471, respectively. Essential oils obtained from all the species were soluble in 95% alcohol at different levels (3-4 ml). While acid value decreased, ester and saponification values of essential oil increased with increasing physiological loss in mass when extracted from fruits stored for increasing duration at both ambient as well as refrigerated conditions. The essential oil from grapefruit had least acid value, while its ester and saponification values were highest indicating its better quality. Quantitatively, the most representative monoterpene was observed to be limonene with concentration of 95.13, 96.23, 93.57, 96.41% in oils of Jatti khatti, Soh sarkar, grapefruit, and pummelo, respectively

Generation and characterization of induced pluripotent stem cell line IITGi001-A derived from adult human primary dermal fibroblasts

Adult human primary dermal fibroblasts [ATCC (PCS-201-012)] were reprogrammed by transfection of oriP/EBNA-1 based episomal plasmids expressing OCT3/4, SOX2, KLF4, L-MYC, LIN28 and a p53 shRNA (Okita et al., 2011) to give rise to induced pluripotent stem cells (iPSCs). These iPSCs expressed core pluripotency markers, maintained normal karyotype, and showed tri-lineage differentiation potential. Further, the absence of episomal plasmid integration in this iPSC line was confirmed by genomic PCR. In addition, DNA fingerprinting of fibroblast and iPSC DNA by microsatellite analysis confirmed the genetic identity of this cell line. This iPSC line was shown to be free from mycoplasma contamination

Biochemistry of Microbial Degradation of Hexachlorocyclohexane and Prospects for Bioremediation

Summary: Lindane, the γ-isomer of hexachlorocyclohexane (HCH), is a potent insecticide. Purified lindane or unpurified mixtures of this and α-, β-, and δ-isomers of HCH were widely used as commercial insecticides in the last half of the 20th century. Large dumps of unused HCH isomers now constitute a major hazard because of their long residence times in soil and high nontarget toxicities. The major pathway for the aerobic degradation of HCH isomers in soil is the Lin pathway, and variants of this pathway will degrade all four of the HCH isomers although only slowly. Sequence differences in the primary LinA and LinB enzymes in the pathway play a key role in determining their ability to degrade the different isomers. LinA is a dehydrochlorinase, but little is known of its biochemistry. LinB is a hydrolytic dechlorinase that has been heterologously expressed and crystallized, and there is some understanding of the sequence-structure-function relationships underlying its substrate specificity and kinetics, although there are also some significant anomalies. The kinetics of some LinB variants are reported to be slow even for their preferred isomers. It is important to develop a better understanding of the biochemistries of the LinA and LinB variants and to use that knowledge to build better variants, because field trials of some bioremediation strategies based on the Lin pathway have yielded promising results but would not yet achieve economic levels of remediation