Response of Chickpea (Cicier Arietinium L.) to Rhizobium Inoculation and Blended fertilizer Rates in Laelay Maichew, Central Zone of Tigray, Northern Ethiopia

Low soil fertility is one of the limiting factor for low productivity of chickpea in Central zone of Tigray, Northern Ethiopia. Field experiment was therefore, conducted for two consecutive years (2016-2017) in Laelay Maichew (Hatsebo) to evaluate the effects of NPSB fertilizer and rhizobium inoculation on yield and yield components of chickpea. The experiment was laid out in a split plot design with three replications. Rhizobium inoculation was assigned to the main plots with two levels (with and without rhizobium inoculation) and NPSB fertilizer rates in sub plot with seven levels (0, 25, 50, 75, 100, 125 and 150 kg ha-1 NPSB). Data collected were subjected to the analysis of variance (ANOVA) using SAS software. A combined analysis of variance showed a significant interaction effects of NPSB and rhizobium inoculation on chickpea yield and yield components (P<0.05) across the two years. The highest number of pods per plant (76.8) was recorded from 125 kg ha‑1 NPSB along with rhizobium inoculation and the highest grain yields were obtained from 150 kg ha‑1 (3609 kg ha‑1) and 125 kg ha‑1 NPSB (3514 kg ha‑1) along with rhizobium inoculation. Maximum marginal rate of return (4106.68%) was gained when chickpea was inoculated with rhizobium and 125 kg ha-1 NPSB application. From the present results it could be concluded that 125 kg ha-1 NPSB along with rhizobium inoculation seeds would be the optimum treatment combination for enhancing chickpea yield and better profitability in soils with low level of available plant nutrients (NPSB)

Population Genomics of Mycobacterium tuberculosis in Ethiopia Contradicts the Virgin Soil Hypothesis for Human Tuberculosis in Sub-Saharan Africa

Colonial medical reports claimed that tuberculosis (TB) was largely unknown in Africa prior to European contact, providing a "virgin soil" for spread of TB in highly susceptible populations previously unexposed to the disease [1, 2]. This is in direct contrast to recent phylogenetic models which support an African origin for TB [3-6]. To address this apparent contradiction, we performed a broad genomic sampling of Mycobacterium tuberculosis in Ethiopia. All members of the M. tuberculosis complex (MTBC) arose from clonal expansion of a single common ancestor [7] with a proposed origin in East Africa [3, 4, 8]. Consistent with this proposal, MTBC lineage 7 is almost exclusively found in that region [9-11]. Although a detailed medical history of Ethiopia supports the view that TB was rare until the 20(th) century [12], over the last century Ethiopia has become a high-burden TB country [13]. Our results provide further support for an African origin for TB, with some genotypes already present on the continent well before European contact. Phylogenetic analyses reveal a pattern of serial introductions of multiple genotypes into Ethiopia in association with human migration and trade. In place of a "virgin soil" fostering the spread of TB in a previously naive population, we propose that increased TB mortality in Africa was driven by the introduction of European strains of M. tuberculosis alongside expansion of selected indigenous strains having biological characteristics that carry a fitness benefit in the urbanized settings of post-colonial Africa

Investigation of the high rates of extrapulmonary tuberculosis in Ethiopia reveals no single driving factor and minimal evidence for zoonotic transmission of Mycobacterium bovis infection

Ethiopia, a high tuberculosis (TB) burden country, reports one of the highest incidence rates of extra-pulmonary TB dominated by cervical lymphadenitis (TBLN). Infection with Mycobacterium bovis has previously been excluded as the main reason for the high rate of extrapulmonary TB in Ethiopia.; Here we examined demographic and clinical characteristics of 953 pulmonary (PTB) and 1198 TBLN patients visiting 11 health facilities in distinct geographic areas of Ethiopia. Clinical characteristics were also correlated with genotypes of the causative agent, Mycobacterium tuberculosis.; No major patient or bacterial strain factor could be identified as being responsible for the high rate of TBLN, and there was no association with HIV infection. However, analysis of the demographic data of involved patients showed that having regular and direct contact with live animals was more associated with TBLN than with PTB, although no M. bovis was isolated from patients with TBLN. Among PTB patients, those infected with Lineage 4 reported "contact with other TB patient" more often than patients infected with Lineage 3 did (OR = 1.6, CI 95% 1.0-2.7; p = 0.064). High fever, in contrast to low and moderate fever, was significantly associated with Lineage 4 (OR = 2.3; p = 0.024). On the other hand, TBLN cases infected with Lineage 4 tended to get milder symptoms overall for the constitutional symptoms than those infected with Lineage 3.; The study suggests a complex role for multiple interacting factors in the epidemiology of extrapulmonary TB in Ethiopia, including factors that can only be derived from population-based studies, which may prove to be significant for TB control in Ethiopia

Barriers in the implementation of isoniazid preventive therapy for people living with HIV in Northern Ethiopia: a mixed quantitative and qualitative study

Abstract Background Isoniazid preventive therapy is a key public health intervention for the prevention of tuberculosis disease among people living with HIV. Despite the confirmed efficacy of isoniazid preventive therapy and global recommendations existing for decades, its implementation remains limited. In resource constrained settings, few have investigated why isoniazid preventive therapy is not implemented on full scale. This study was designed to investigate the level of isoniazid preventive therapy implementation and reasons for suboptimal implementation in Tigray region of Ethiopia. Methods A review of patient records combined with a qualitative study using in-depth interviews and focus group discussions was conducted in 11 hospitals providing isoniazid preventive therapy in the Tigray Region. The study participants were health providers working in the HIV clinics of the 11 hospitals in the province. Health providers were interviewed about their experience of providing isoniazid preventive therapy and challenges faced during its implementation. All conversations were audio-recorded. Record review of 16,443 HIV patients registered for care in these hospitals between September 2011 and April 2014 was done to determine isoniazid preventive therapy utilization. Data were collected from April to August 2014. Results Fifty health providers participated in the study. Overall isoniazid preventive therapy coverage of the region was estimated to be 20 %. Isoniazid stock out, fear of creating isoniazid resistance, problems in patient acceptance, and lack of commitment of health managers to scale up the program were indicated by health providers as the main barriers hindering implementation of isoniazid preventive therapy. Conclusion Implementation of isoniazid preventive therapy in Tigray region of Ethiopia had low coverage. Frequent interruption of isoniazid supplies raises the concern of interrupted therapy resulting in creation of isoniazid resistance. Health managers, drug suppliers and partners working in HIV and tuberculosis programs should be committed to ensure an uninterrupted supply of isoniazid and full scale implementation of isoniazid preventive therapy to eligible people living with HIV

Nutritive and Chemical Composition and In Vitro Digestibility of Cladodes of the <i>Opuntia</i> Species

Sixteen cultivars (three resistant species and thirteen susceptible cultivars to cochineal insects) were included in the experiment to evaluate their nutritional and chemical compositions and to determine the in vitro digestibility of cladodes of the Opuntia species. Cultivars showed highly significant (p 0.001) differences in their chemical composition. The cultivars’ content of ash, crude protein (CP), dry matter (DM), organic matter (OM), in vitro dry matter digestibility (IVDMD), in vitro organic matter digestibility (IVOMD), acid detergent fiber (ADF), neutral detergent fiber (NDF) and Cell (cellulose) varied, with ranges of 8.18–22.75%, 3.19–10.40%, 86.40–91.88%, 77.45–91.82%, 68.25–87.3%, 55.81–78.95%, 14.88–26.15%, 29.66–67.05% and 10.99–19.92%, respectively. The ash content showed a significant highly negative correlation with OM (r = −1.00; p p p O. robusta var. X11 cultivars showed the highest similarities, as they are found close to the first branch of the dendrogram. In total, 90% of the variation among the nutritional traits was attributed to the first four PCs, with 55.97% to the first two principal components (PCs). The nutritional composition of the resistant species was greater than or equal to the susceptible ones

High Prevalence of Bovine Tuberculosis in Dairy Cattle in Central Ethiopia:Implications for the Dairy Industry and Public Health

BACKGROUND: Ethiopia has the largest cattle population in Africa. The vast majority of the national herd is of indigenous zebu cattle maintained in rural areas under extensive husbandry systems. However, in response to the increasing demand for milk products and the Ethiopian government's efforts to improve productivity in the livestock sector, recent years have seen increased intensive husbandry settings holding exotic and cross breeds. This drive for increased productivity is however threatened by animal diseases that thrive under intensive settings, such as bovine tuberculosis (BTB), a disease that is already endemic in Ethiopia. METHODOLOGY/PRINCIPAL FINDINGS: AN EXTENSIVE STUDY WAS CONDUCTED TO: estimate the prevalence of BTB in intensive dairy farms in central Ethiopia; identify associated risk factors; and characterize circulating strains of the causative agent, Mycobacterium bovis. The comparative intradermal tuberculin test (CIDT), questionnaire survey, post-mortem examination, bacteriology, and molecular typing were used to get a better understanding of the BTB prevalence among dairy farms in the study area. Based on the CIDT, our findings showed that around 30% of 2956 tested dairy cattle from 88 herds were positive for BTB while the herd prevalence was over 50%. Post-mortem examination revealed gross tuberculous lesions in 34/36 CIDT positive cattle and acid-fast bacilli were recovered from 31 animals. Molecular typing identified all isolates as M. bovis and further characterization by spoligotyping and MIRU-VNTR typing indicated low strain diversity within the study area. CONCLUSIONS/SIGNIFICANCE: This study showed an overall BTB herd prevalence of 50% in intensive dairy farms in Addis Ababa and surroundings, signalling an urgent need for intervention to control the disease and prevent zoonotic transmission of M. bovis to human populations consuming dairy products coming from these farms. It is suggested that government and policy makers should work together with stakeholders to design methods for the control of BTB in intensive farms in Ethiopi

Investigation of the high rates of extrapulmonary tuberculosis in Ethiopia reveals no single driving factor and minimal evidence for zoonotic transmission of Mycobacterium bovis infection

Abstract Background: Ethiopia, a high tuberculosis (TB) burden country, reports one of the highest incidence rates of extra-pulmonary TB dominated by cervical lymphadenitis (TBLN). Infection with Mycobacterium bovis has previously been excluded as the main reason for the high rate of extrapulmonary TB in Ethiopia

Mycobacterial lineages causing pulmonary and extrapulmonary Tuberculosis, Ethiopia

Molecular typing of 964 specimens from patients in Ethiopia with lymph node or pulmonary tuberculosis showed a similar distribution of Mycobacterium tuberculosis strains between the 2 disease manifestations and a minimal role for M. bovis. We report a novel phylogenetic lineage of M. tuberculosis strongly associated with the Horn of Africa