Linguocultural model of description of demonological vocabulary

The article deals with the complex linguocultural model of description of demonological vocabulary, which includes structural semantic, onomasiological, psycholinguistical and ethnolinguistical aspects. Different nominations of the goblin are analyzed in the article.Рассматривается комплексная лингвокультурологическая модель описания демонологической лексики, включающая в себя структурно-семантический, омонасиологический, психолингвистический и этнолингвистический аспекты. В качестве примера анализируются номинации домового

Structure of lexical meaning of dialect demonologeme

Different variants of describing semantic structure of a word connected with singling out different set of components are summarized. The algorithm of describing semantic structure of demonologeme, which is characterized by a virtual denotate, disposability to create connotative (pejorative) meanings, importance of syntagmatic components of semantics; usage of algorithm is demonstrated on the basis of several demonologemes.Обобщаются различные варианты описания семантической структуры слова, связанные с выделением разного набора компонентов. Приводится алгоритм описания семантической структуры демонологемы, характеризующейся виртуальным денотатом, склонностью к образованию коннотативных (главным образом пейоративных) значений, важностью синтагматических компонентов семантики; использование алгоритма демонстрируется на примере нескольких демонологем

PHYSICAL DEVELOPMENT INDEX METHOD ASSESSMENT OF IRKUTSK PRESCHOOL AGE CHILDREN

189 preschool age children (3-6 y.o.) of Irkutsk have been surveyed. By means of a centile method all the surveyed children have been divided into three groups according to their physical development (PD): the 1st group - children with harmonious PD, the 2nd group - children with disharmonious PD due to body weight deficiency, the 3rd group - children with disharmonious Pd due to body weight surplus. Results of research revealed PD deterioration, which was expressed in body weight increase in children of 3-6 years of age. Index method PD assessment showed that calculations received with this method do not correspond with groups of harmony of preschool children physical development

Methodology of teaching propositionson classes on the Russian language as a foreign language

The article deals with the problem of teaching methods of Russian prepositions in the process of teaching Russian as a foreign language to students of Chinese universities. Review of the literature of both Russian and Chinese linguists dealing with issues related to prepositions is given. Based on the theory of prototypes and the theory of the metaphor of space, the authors show the importance of studying the many-valued semantics of prepositions. Due to the high frequency of the use of prepositions in the Russian language, foreign students have difficulties in distinguishing their meanings, therefore the proposed three-step method of studying Russian prepositions contributes to better memorization and proper use of them in speech.В статье рассматривается проблема методики преподавания русских предлогов в процессе обучения русскому языку как иностранному студентов китайских вузов

Quantitative analysis of the benzanthrone aminoderivative binding to amyloid fibrils of lysozyme

The accumulation of amyloid fibrils in different tissues is associated with a number of neurodegenerative diseases. Despite a huge variety of amyloid-specific probes, all of them suffer from many drawbacks, highlighting the necessity of searching for more preferable dyes. In the present work, the potential of new fluorescent probe AM3 for selective detection of fibrillar protein aggregates, formed from lysozyme, has been evaluated. To quantify the affinity of this dye for amyloid fibrils, the isotherms of dye binding to the fibrillar lysozyme have been derived from fluorimetric titration. Parameters of the dye-protein complexation: association constant, molar fluorescence and binding stoichiometry, calculated from the Langmuir adsorption model, revealed that AM3 interacts strongly with protein insoluble aggregates. High values of the binding parameters make AM3 an alternative to a widely-used amyloid-specific probe Thioflavin T. We also investigated the effects of polarity and viscosity on AM3 fluorescence properties. The binding of AM3 to the protein hydrophobic cavities has been followed by red shift of the dye emission spectra, which can be explained by H-bonding between proton-donating groups of the protein and carbonyl moiety of the probe. Long-wavelength shift of emission maximum was observed also upon increasing the excitation wavelength. This finding suggests that reorientation time of solvent molecules is higher, than the dye fluorescence lifetime. Fluorescence anisotropy studies revealed slow rotation diffusion of the probe, bound to amyloid fibrils being indicative of high viscosity of AM3 microenvironment. The observed photophysical properties of the new aminobenzanthrone derivative make AM3 a perspective probe for basic research and medical diagnostics

Floral complexes with the involvement of Adonis Vernalis L. (fam. Ranunculaceae juss.) and environmental assessment of the conditions of their formation in the Southwest of Central-Russian Upland

The article presents the results of the study of structures of floral complexes with the involvement of Adonis vernalis L., the number of genera, families and species is indicated. Average points of severity of environmental factors were calculated, allowing assessing the environmental conditions in these ecotopes. Ecobiomorphs of species of the floral complexes in natural and quasi-natural ecotops are determine

Mutants of Taq DNA polymerase resistant to PCR inhibitors allow DNA amplification from whole blood and crude soil samples

Potent PCR inhibitors in blood and soil samples can cause false negative results from PCR-based clinical and forensic tests. We show that the effect of these inhibitors is primarily upon Taq DNA polymerase, since mutational alteration of the polymerase can overcome the inhibition to the extent that no DNA purification is now required. An N-terminal deletion (Klentaq1) is some 10–100-fold inhibition resistant to whole blood compared to full-length, wild-type (w.t.) Taq, which is strongly inhibited by 0.1–1% blood. Further mutations at codon 708, both in Klentaq 1 and Taq, confer enhanced resistance to various inhibitors of PCR reactions, including whole blood, plasma, hemoglobin, lactoferrin, serum IgG, soil extracts and humic acid, as well as high concentrations of intercalating dyes. Blood PCR inhibitors can predominantly reduce the DNA extension speed of the w.t. Taq polymerase as compared to the mutant enzymes. Single-copy human genomic targets are readily amplified from whole blood or crude soil extract, without pretreatment to purify the template DNA, and the allowed increase in dye concentration overcomes fluorescence background and quenching in real-time PCR of blood

Use of settlement indexes for an assessment of physical development of preschool children of the city of Irkutsk

189 children of preschool age of 3-6 years of the city of Irkutsk are examined. The assessment of FR of children by means of a method of indexes, showed discrepancy of calculations by this method with groups of a harmony of FR of the preschool children examined by method the tsentilnykh of tablesОбследовано 189 детей дошкольного возраста 3-6 лет города Иркутска. Результаты исследования выявили увеличение массы тела дошкольников. Оценка ФР детей с помощью метода индексов, показала несоответствие расчетов данным методом с группами гармоничности ФР дошкольников, обследованных методом центильных табли