Additional file 2: Figure S2. of Targeting of tubulin polymerization and induction of mitotic blockage by Methyl 2-(5-fluoro-2-hydroxyphenyl)-1H-benzo[d]imidazole-5-carboxylate (MBIC) in human cervical cancer HeLa cell

Molecular docking of MBIC with tubulin complex. Hydrogen bonds between amino acids of tubulin complex and MBIC. Molecular structure was visualized by UCSF Chimera 1.8.1 program. Figure (A) is showing ribbon representation of tubulin-MBIC complex. Figure (B) is showing hydrogen bonds (green line) of MBIC with surrounding amino acids residues which are included polar lysine 352 and hydrophobic valine 181 amino acids. (PDF 3179 kb

Additional file 1: Figure S1. of Targeting of tubulin polymerization and induction of mitotic blockage by Methyl 2-(5-fluoro-2-hydroxyphenyl)-1H-benzo[d]imidazole-5-carboxylate (MBIC) in human cervical cancer HeLa cell

MBIC displayed synergistic effect with selected conventional drugs. A. shows isobolograms that link two concentrations of combined drug in their equal activity points (inhibition). In this isobolograms, we can see equal effect (50 %, 75 %, and 90 % cytotoxicity) points of MBIC in combination with indicated conventional drugs. B. is fractional affected-combination index (Fa-CI) plots that show 0 to 100 % inhibitions of MBIC combined with indicated conventional drugs in quantitatively definition of effect (CI) value. The CI value is lower than 1 represents the synergistic effect of MBIC in combination with a conventional drug, wherein their overall effect is higher than the effect of each individual drug. CI vale equals 1, represents the additive or indifferent effect of MBIC in combination with conventional drugs. CI value above 1 represents the antagonistic effect of MBIC in combination with conventional drugs wherein their overall effect is less than the effect of each individual drug. (PDF 207 kb