Reflective Perspectives of Host and Guest Participants of an International Professional Development Experience

Global competence is a skill set that has become a necessity in every discipline in the agricultural sciences. With an increasingly diverse student population in our school systems coupled with an increasingly complex global agrisystem, now more than ever we need globally competent educators and learners to develop a globally competent workforce and society. A common intervention in cultivating global competency is the study abroad immersive experience for the traveling participants, but little is known about the global competency gains in hosting individuals. The immersive study abroad experience provided the opportunity for school-based agricultural educators (both candidates and practicing educators) to engage in a four-week experience with their Malaysian counterparts exploring the interconnected nature of culture, agriculture, and education. Throughout the experience the participants engaged in daily structured reflection sessions using the TIPS method. Journal entries were coded and analyzed for both Malaysia and United States participants. Participants from both countries respectively became more globally competent educators and learners

Recommendations for the Generation, Quantification, Storage, and Handling of Peptides Used for Mass Spectrometry-Based Assays

BACKGROUND: For many years, basic and clinical researchers have taken advantage of the analytical sensitivity and specificity afforded by mass spectrometry in the measurement of proteins. Clinical laboratories are now beginning to deploy these work flows as well. For assays that use proteolysis to generate peptides for protein quantification and characterization, synthetic stable isotope-labeled internal standard peptides are of central importance. No general recommendations are currently available surrounding the use of peptides in protein mass spectrometric assays. CONTENT: The Clinical Proteomic Tumor Analysis Consortium of the National Cancer Institute has collaborated with clinical laboratorians, peptide manufacturers, metrologists, representatives of the pharmaceutical industry, and other professionals to develop a consensus set of recommendations for peptide procurement, characterization, storage, and handling, as well as approaches to the interpretation of the data generated by mass spectrometric protein assays. Additionally, the importance of carefully characterized reference materials-in particular, peptide standards for the improved concordance of amino acid analysis methods across the industry-is highlighted. The alignment of practices around the use of peptides and the transparency of sample preparation protocols should allow for the harmonization of peptide and protein quantification in research and clinical care