Assessment of a New Elbow Joint Positioning Method Using Area Detector Computed Tomography

We propose a sitting position that achieves both high image quality and a reduced radiation dose in elbow joint imaging by area detector computed tomography (ADCT), and we compared it with the ‘superman’ and supine positions. The volumetric CT dose index (CTDIvol) for the sitting, superman, and supine positions were 2.7, 8.0, and 20.0 mGy and the dose length products (DLPs) were 43.4, 204.7, and 584.8 mGy • cm, respectively. In the task-based transfer function (TTF), the highest value was obtained for the sitting position in both bone and soft tissue images. The noise power spectrum (NPS) of bone images showed that the superman position had the lowest value up to approx. 1.1 cycles/mm or lower, whereas the sitting position had the lowest value when the NPS was greater than approx. 1.1 cycles/mm. The overall image quality in an observer study resulted in the following median Likert scores for Readers 1 and 2: 5.0 and 5.0 for the sitting position, 4.0 and 3.5 for the superman position, and 4.0 and 2.0 for the supine position. These results indicate that our proposed sitting position with ADCT of the elbow joint can provide superior image quality and allow lower radiation doses compared to the superman and supine positions

Working together in State-Building and Dvelopment

This volume illustrates the stories of a great number of Japanese who have worked to support reconstruction and development in Afghanistan, with a focus on the ten years since 2001, After the September 11 terrorist attacks in the United States in 2001, the international community has collectively supported a new state-building in Afghanistan. Japan, as part of the community, has been providing support, drawing on its unique expertise and experience. Many Japanese have endeavored to help local Afghans return to peaceful daily lives and to support the state’s reconstruction and development, sometimes with taking personal risks. The book, introducing their daily activities alongside the accomplishment of their painstaking work, attempts to recapture the overall picture of Japan’s support for Afghanistan. In preparing the volume, the JICA-RI established a project team to compile the experiences and information from a variety of people―representatives of the Japanese government and the private sector, NGO members, staff of UN organizations and JICA―who have involved in the Afghan reconstruction in various positions. The project team reproduced a concrete picture of their activities in Afghanistan. The book presents a wide range of efforts: the emergency humanitarian aid during the initial stage of reconstruction; the assistance for a new state institution building from scratch; the support to build foundation for people’s livelihoods; and the human resource development for the future of Afghanistan. The volume also introduces a number of episodes on Japanese assistance in the 1970s. After the twenty-year absence during the war (in the 1980s and 1990s) their impacts still remains in various places even today. These episodes remind us of the importance of assistance to develop human resources and infrastructure that will serve as the foundation for state-building over decades

Functional Characterization of the Receiver Domain for Phosphorelay Control in Hybrid Sensor Kinases.

Hybrid sensor kinase, which contains a histidine kinase (HK) domain, a receiver domain, and a histidine-containing phosphotransmitter (HPt) domain, conveys signals to its cognate response regulator by means of a His-Asp-His-Asp phosphorelay. We examined the multistep phosphorelay of a recombinant EvgAS system in Escherichia coli and performed in vitro quantitative analyses of phosphorylation by using Phos-tag SDS-PAGE. Replacement of Asp in the receiver domain of EvgS by Ala markedly promoted phosphorylation at His in the HK domain compared with that in wild-type EvgS. Similar Ala-substituted mutants of other hybrid sensor kinases BarA and ArcB showed similar characteristics. In the presence of sufficient ATP, autophosphorylation of the HK domain in the mutant progressed efficiently with nearly pseudo-first-order kinetics until the phosphorylation ratio reached a plateau value of more than 95% within 60 min, and the value was maintained until 180 min. However, both wild-type EvgS and the Ala-substituted mutant of His in the HPt domain showed a phosphorylation ratio of less than 25%, which gradually decreased after 10 min. These results showed that the phosphorylation level is regulated negatively by the receiver domain. Furthermore, our in vivo assays confirmed the existence of a similar hyperphosphorylation reaction in the HK domain of the EvgS mutant in which the Asp residue was replaced with Ala, confirming the validity of the control mechanism proposed from profiling of phosphorylation in vitro [corrected]

Establishment of Down’s syndrome periodontal ligament cells by transfection with SV40T-Ag and hTERT

Down’s syndrome is one of the most common human congenital genetic diseases and affected patients have increased risk of periodontal disease. To examine involvement of the disease with periodontal disease development, we established immortalized periodontal ligament cells obtained from a Down’s syndrome patient by use of SV40T-Ag and hTERT gene transfection. Expressions of SV40T-Ag and hTERT were observed in periodontal ligament cell-derived immortalized cells established from healthy (STPDL) and Down’s syndrome patient (STPDLDS) samples. Primary cultured periodontal ligament cells obtained from a healthy subject (pPDL) had a limited number of population doublings (< 40), while STPDL and STPDLDS cells continued to grow with more than 80 population doublings. Primary cultured periodontal ligament cells obtained from the patient showed a chromosome pattern characteristic of Down’s syndrome with trisomy 21, whereas STPDLDS samples showed a large number of abnormal chromosomes in those results. Gene expression analysis revealed that expression of DSCR-1 in STPDLDS is greater than that in STPDL. These results suggest that the newly established STPDLDS cell line may be a useful tool for study of periodontal disease in Down’s syndrome patients

The origin of seeping gas from the coastal area of Yagaji Island in Northern Okinawa Island

Chemical and isotopic compositions of seeping gas from the coastal area of Yagaji Island were measured. The gases composed mainly of methane. The δ13C and δD of methane suggest that the origin of the methane is thermal decomposition of organic matter. The δ13C of methane and CH4/(C2H6+C3H8) ratio in the gases suggest long distance migration from the sources. The 3He/4He ratios in the Yagaji gases suggest thermal decomposition of organic matter occurs in deeply-buried sediments. The Yagaji gases would be generated from marine organic matter in the Motobu group around 4 km below the ground

Autophosphorylation reactions of other hybrid sensor kinases, BarA and ArcB.

<p>(A) Phosphorylation sites in each domain of BarA and ArcB are shown. (B) Autophosphorylation reactions of wild-type BarA (WT) and the two mutants D718A and H861A were performed in the presence of 10 mM ATP, and the products were then analyzed by Phos-tag SDS-PAGE. The incubation times are shown above each lane. Each lane contained 2 μg of protein. Bands for each of the two site-specific phosphorylated forms were assigned and are shown by arrows on the right-hand side of the panel. (C) Autophosphorylation reactions of wild-type ArcB (WT) and the two mutants D576A and H717A were performed and analyzed in the same manner as BarA in B. (D) Values of the ratio of the phosphorylated forms to the total proteins in B and C were calculated by densitometry and are plotted versus the reaction times. Each plot was gained as average of three independent experiments using the same sample. Standard deviations were within almost 20%.</p