Functional consequences of the oligomeric assembly of proteorhodopsin.

The plasma membrane is the crucial interface between the cell and its exterior, packed with embedded proteins experiencing simultaneous protein-protein and protein-membrane interactions. A prominent example of cell membrane complexity is the assembly of transmembrane proteins into oligomeric structures, with potential functional consequences that are not well understood. From the study of proteorhodopsin (PR), a prototypical seven-transmembrane light-driven bacterial proton pump, we find evidence that the inter-protein interaction modulated by self-association yields functional changes observable from the protein interior. We also demonstrate that the oligomer is likely a physiologically relevant form of PR, as crosslinking of recombinantly expressed PR reveals an oligomeric population within the Escherichia coli membrane (putatively hexameric). Upon chromatographic isolation of oligomeric and monomeric PR in surfactant micelles, the oligomer exhibits distinctly different optical absorption properties from monomeric PR, as reflected in a prominent decrease in the pKa of the primary proton acceptor residue (D97) and slowing of the light-driven conformational change. These functional effects are predominantly determined by specific PR-PR contacts over nonspecific surfactant interactions. Interestingly, varying the surfactant type alters the population of oligomeric states and the proximity of proteins within an oligomer, as determined by sparse electron paramagnetic resonance distance measurements. Nevertheless, the dynamic surfactant environment retains the key function-tuning property exerted by oligomeric contacts. A potentially general design principle for transmembrane protein function emerges from this work, one that hinges on specific oligomeric contacts that can be modulated by protein expression or membrane composition

Multinational Teams: The Relationship Between Work Values and Productivity and the Moderating Effect of Degree of Operational Integration

The present study gathered data from 147 employees in two U.S. headquartered multinational manufacturing companies. Participants responded to questions about their work-related values, perceptions of the productivity of their work teams, and the degree of operational integration between firms‟ foreign affiliate offices and their U.S. based headquarters. Additionally, analyses were conducted to assess the relationship between team productivity and psychic distance, which is a measure of country-level cultural differences. Work values were assessed using The Values Scale (Neville & Super, 1989), perceptions of team productivity were assessed used an instrument by Kirkman and Rosen (1999), degree of operational integration was measured using a set of items developed by Slangen (2006), and psychic distance was assessed using a scale created by Hakanson and Ambos (2010). As expected, work values were found to be different between a firm‟s headquarters and a foreign office and work values predicted perceptions of team productivity when controlling for the age of the team. Psychic distance was found to predict team productivity when team age was controlled, and a more highly integrated degree of operational integration was found to relate to perceived productivity. Contrary to expectation, a significant interaction was not found to exist between work values, degree of integration, and psychic distance. However, a significant interaction was found between psychic distance and work values. Implications of the findings, limitation of the data, and directions for future research are also presented

The Unvoiced Barriers of African American Females Who Did Not Persist to Graduation from a Predominantly White Technical College: A Phenomenological Study

The purpose of this qualitative transcendental phenomenological study was to describe the lived experiences of African American female past students, regarding unvoiced barriers, at Seven Hills Technical College. The theory guiding this study was Tinto’s theory on student integration. Tinto believed that a student’s academic and social interactions are indicators of whether a student will be successful. The interpretive framework utilized in this study was critical race theory. The central research question guiding this study was: What are the lived experiences of African American female past students who did not persist to graduation from a predominantly White technical college? The sample included African American female past students who did not successfully persist to graduation. Data was collected using semi-structured interviews, document analysis, and a focus group. The analysis strategy process involved Moustakas’ seven-step thematic analysis method. After an in-depth analytical review, three themes were revealed. These themes include self-improvement, unpreparedness, and identity. Overall, utilizing the qualitative transcendental phenomenological approach allowed the researcher, participants, and the audience a space to understand the lived experiences of African American female past students

Playing Back Spirituality: Using Applied Theatre Practice for Spiritual Exploration an Meaningful Community Building in College

Higher Education in the U.S. today is experiencing a theoretical and practical shift toward educating the whole person and to that end, is investigating ways to include spirituality in all facets of the academy. This requires focusing on the concept that in addition to material knowledge, spiritual, emotional, and ethical knowledge is imparted to students (Khan 2009). Many colleges and universities are searching for avenues to answer this call and better prepare students as business, political and social leaders in a new millennia that defines religion very differently than it did twenty years ago. Today, students are exposed to a much wider array of organized religions from all over the globe. Additionally, the very definitions of religion and spirituality have undergone a seismic shift making it difficult for colleges to incorporate a religious or spiritual focus into curriculum. More and more people are cobbling together their own unique combinations of religious ideas, practices, experiences and core values from a variety of religious and non-religious sources. The term \u27spirituality\u27 is sometimes used to describe this new do-it-yourself faith...To be \u27spiritual\u27 understood in this sense, is to have deeply held convictions, and anyone can have those kinds of heartfelt allegiances. This new ambiguity about what counts as religion or spirituality makes it virtually impossible to keep religion out of higher education. (Jacobsen and Jacobsen 2012) Research on the subject of religion and spirituality indicates that this is a point of major concern for many young American college students who are searching for personal and social significance. (Arnett 2000a; Astin, et al. 2011; Jacobsen and Jacobsen 2012) This study asserts that college students enter a unique stage of development known as emerging adulthood (Arnett 2000a) that calls for increased focus on meaning making and identity formation. In an effort to meet the individual and institutional need for spiritual exploration, this study will offer specific applied theatre practices that connect theories in theatre, psychology, student development and leadership designed to serve the emerging adult population as part of a holistic educational vision. This study confirms the feasibility of such a program by a detailed examination of specific theatre techniques and, in particular, the adaptation of Playback Theatre as the most viable form for inner life exploration and campus community building. A formal investigation into the efficacy of theatrical methods is called for as validation of theories and practices offered here. It is my hope that this research will encourage campus-wide awareness of theatre\u27s utility and application to a wider range of students. By recognizing the need to educate the whole person , institutions of Higher Education can give students the best possible preparation for a full and meaningful adult life through theatre practices uniquely designed for the purpose of inner life exploration and awareness. Key Implications: new areas of application for Applied Theatre Studies; collaborative opportunities for college theatre departments and student services, expansion of campus wide-visibility and understanding of theatre arts, feasibility for attending to student inner life needs and student community building through theatre

Approaches to interdisciplinary mixed methods research in land change science and environmental management

Combining qualitative and quantitative methods and data is crucial to understanding the complex dynamics and often interdisciplinary nature of conservation. Many conservation scientists use mixed methods, but there are a variety of mixed methods approaches, a lack of shared vocabulary, and few methodological frameworks. We reviewed articles from 2 conservation-related fields that often incorporate qualitative and quantitative methods: land-change science (n= 16) and environmental management (n= 16). We examined how authors of these studies approached mixed-methods research by coding key methodological characteristics, including relationships between method objectives, extent of integration, iterative interactions between methods, and justification for use of mixed methods. Using these characteristics, we created a typology with the goal of improving understanding of how researchers studying land-change science and environmental management approach interdisciplinary mixed methods research. We found 5 types of mixed methods approaches, which we termed simple nested, informed nested, simple parallel, unidirectional synthesis, and bidirectional synthesis. Methods and data sources were often used to address different research questions within a project, and only around half of the reviewed papers methodologically integrated different forms of data. Most authors used one method to inform the other rather than both informing one another. Very few articles used methodological iteration. Each methodological type has certain epistemological implications, such as the disciplinary reach of the research and the capacity for knowledge creation through the exchange of information between distinct methodologies. To exemplify a research design that can lead to multi-dimensional knowledge production, we provide a methodological framework that bidirectionally integrates and iterates qualitative and quantitative methods

Differing short-term impacts of agricultural tarping on soil-dwelling and surface-active arthropods

Agricultural tarping, the practice of placing impermeable plastic tarps over crop beds before planting to suppress weeds, is rising in popularity. However, the use of tarps has uncertain effects on soil arthropod communities. We studied the impact of silage (black plastic) tarps and clear plastic tarps on surface-active and soil-dwelling arthropods by tracking immediate impacts and arthropod recovery for 5 weeks after tarps were removed. We also assessed how well environmental and experimental variables explained arthropod diversity and composition. During tarp application, we found that both silage and clear plastic tarps had significant negative impacts on surface-active arthropod diversity, while only clear plastic tarps impacted soil-dwelling arthropods. Surface-active arthropod diversity recovered by 1-3 weeks after tarping, but at 5 weeks after tarping soil-dwelling arthropod diversity was significantly lower in silage tarp and clear plastic plots than control plots. Tarps also led to compositional changes in the arthropod communities, though these changes were only significant during tarp cover. The variables that best explained arthropod diversity and community composition were treatment (i.e., silage tarp, clear plastic tarp, or control) during tarping and farm site after tarps were removed. Other variables, such as soil moisture and weed coverage, were not strong model predictors. These results imply that tarps may have temporary impacts on surface-active arthropods but potentially longer-lasting impacts on soil-dwelling arthropods. Continuing to monitor impacts on tarps on soil arthropods will better inform the sustainability of this practice

IODNE: An integrated optimization method for identifying the deregulated subnetwork for precision medicine in cancer

Subnetwork analysis can explore complex patterns of entire molecular pathways for the purpose of drug target identification. In this article, the gene expression profiles of a cohort of patients with breast cancer are integrated with protein-protein interaction (PPI) networks using, simultaneously, both edge scoring and node scoring. A novel optimization algorithm, integrated optimization method to identify deregulated subnetwork (IODNE), is developed to search for the optimal dysregulated subnetwork of the merged gene and protein network. IODNE is applied to select subnetworks for Luminal-A breast cancer from The Cancer Genome Atlas (TCGA) data. A large fraction of cancer-related genes and the well-known clinical targets, ER1/PR and HER2, are found by IODNE. This validates the utility of IODNE. When applying IODNE to the triple-negative breast cancer (TNBC) subtype data, we identified subnetworks that contain genes such as ERBB2, HRAS, PGR, CAD, POLE, and SLC2A1

Identification of Alternatively-Activated Pathways between Primary Breast Cancer and Liver Metastatic Cancer Using Microarray Data

Alternatively-activated pathways have been observed in biological experiments in cancer studies, but the concept had not been fully explored in computational cancer system biology. Therefore, an alternatively-activated pathway identification method was proposed and applied to primary breast cancer and breast cancer liver metastasis research using microarray data. Interestingly, the results show that cytokine-cytokine receptor interaction and calcium signaling were significantly enriched under both conditions. TGF beta signaling was found to be the hub in network topology analysis. In total, three types of alternatively-activated pathways were recognized. In the cytokine-cytokine receptor interaction pathway, four active alteration patterns in gene pairs were noticed. Thirteen cytokine-cytokine receptor pairs with inverse activity changes of both genes were verified by the literature. The second type was that some sub-pathways were active under only one condition. For the third type, nodes were significantly active in both conditions, but with different active genes. In the calcium signaling and TGF beta signaling pathways, node E2F5 and E2F4 were significantly active in primary breast cancer and metastasis, respectively. Overall, our study demonstrated the first time using microarray data to identify alternatively-activated pathways in breast cancer liver metastasis. The results showed that the proposed method was valid and effective, which could be helpful for future research for understanding the mechanism of breast cancer metastasis