Construction and Application of a Korean Reference Panel for Imputing Classical Alleles and Amino Acids of Human Leukocyte Antigen Genes

Genetic variations of human leukocyte antigen (HLA) genes within the major histocompatibility complex (MHC) locus are strongly associated with disease susceptibility and prognosis for many diseases, including many autoimmune diseases. In this study, we developed a Korean HLA reference panel for imputing classical alleles and amino acid residues of several HLA genes. An HLA reference panel has potential for use in identifying and fine-mapping disease associations with the MHC locus in East Asian populations, including Koreans. A total of 413 unrelated Korean subjects were analyzed for single nucleotide polymorphisms (SNPs) at the MHC locus and six HLA genes, including HLA-A, -B, -C, -DRB1, -DPB1, and -DQB1. The HLA reference panel was constructed by phasing the 5,858 MHC SNPs, 233 classical HLA alleles, and 1,387 amino acid residue markers from 1,025 amino acid positions as binary variables. The imputation accuracy of the HLA reference panel was assessed by measuring concordance rates between imputed and genotyped alleles of the HLA genes from a subset of the study subjects and East Asian HapMap individuals. Average concordance rates were 95.6% and 91.1% at 2-digit and 4-digit allele resolutions, respectively. The imputation accuracy was minimally affected by SNP density of a test dataset for imputation. In conclusion, the Korean HLA reference panel we developed was highly suitable for imputing HLA alleles and amino acids from MHC SNPs in East Asians, including Koreans

Rudimentary meningocele: remnant of a neural tube defect?

Background: Rudimentary meningocele, a malformation in which meningothelial elements are present in the skin and subcutaneous tissue, has been described in the past, under a variety of different terms and has also been referred to as cutaneous meningioma. There has been debate as to whether rudimentary meningocele is an atretic form of meningocele or results from growth of meningeal cells displaced along cutaneous nerve

A highly hydrophilic water-insoluble nanofiber composite as an efficient and easily-handleable adsorbent for the rapid adsorption of cesium from radioactive wastewater

Herein, we report a new Prussian blue nanoparticle (PBNPs) incorporated polyvinyl alcohol (PVA) composite nanofiber (c-PBNPs/PVA) for the rapid adsorption of cesium (Cs) from radioactive wastewater. Initially, various electrospinning parameters such as solvent, PVA wt%, PBNPs wt% and glutaraldehyde (GA) wt% were extensively optimized to obtain a better physicochemical property of the c-PBNPs/PVA. In order to improve the water insoluble nature of the PVA, post cross-linking was carried out for the c-PBNPs/PVA using glutaraldehyde (GA) and HCl vapor as the cross-linker and catalyst, respectively. SEM images revealed the smooth and continuous morphology of the c-PBNPs/PVA composite nanofibers with diameters of 200–300 nm and lengths up to several millimeters. TEM images confirmed homogeneous dispersion and good incorporation of PBNPs into the PVA matrix. The amorphous nature of the c-PBNPs/PVA was confirmed by the XRD analysis. FT-IR spectra showed successful cross-linking of PVA with GA. It was found that the prepared composite nanofiber is highly hydrophilic and water-insoluble. The c-PBNPs/PVA showed an excellent and faster Cs adsorption rate of 96% after only 100 min. These results are comparable to those previously reported. After the Cs adsorption test, the c-PBNPs/PVA composite nanofiber can be easily separated from the wastewater.ArticleRSC ADVANCES. 4(103):59571-59578 (2014)journal articl

Chylothorax in Gorham's disease.

A 25-yr-old woman presented with a right pleural effusion. Destruction of 9th through 12th ribs, adjacent vertebral bodies, and transverse processes was noted on plain radiograph and a large low-attenuated, irregular shaped mass lesion with peripheral rim enhancement, destroying vertebral body and transverse process, was revealed on the computed tomographic scan. Magnetic resonance imaging showed high signal on T1- weighted image and iso- and low signal on T2-weighted image for the mass lesion replacing the vertebral bony cortex and marrow space. An open rib biopsy revealed the histopathological changes of Gorham's disease (essential osteolysis), even though only bloody fluid filling the empty space and rib and vertebral transverse process destruction were grossly observed on operation. Even though there was no definite response to radiotherapy and pleurodesis, the patient showed stable condition up to 20 months after diagnosis

Effects of Korean Red Ginseng (Panax ginseng), urushiol (Rhus vernicifera Stokes), and probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus acidophilus R0052) on the gut–liver axis of alcoholic liver disease

AbstractBackgroundRoles of immune reaction and toll-like receptor-4 (TLR-4) have widely been established in the pathogenesis of alcoholic liver disease (ALD).MethodsWe evaluated the biologic efficacy of Korean Red Ginseng (KRG), urushiol, and probiotics (Lactobacillus rhamnosus R0011 and Lactobacillus acidophilus R0052) in mouse models of ALD. Sixty C57BL/6 mice were equally divided into six feeding groups for 10 weeks: normal diet, alcohol, control, alcohol + KRG, alcohol + urushiol, and alcohol + probiotics. Alcohol was administered via a Lieber–DeCarli liquid diet containing 10% alcohol. TLR-4 expression, proinflammatory cytokines, and histology, as well as the results of liver function tests were evaluated and compared.ResultsNo between-group differences were observed with regard to liver function. TLR-4 levels were significantly lower in the KRG, urushiol, and probiotics groups than in the alcohol group (0.37 ± 0.06 ng/mL, 0.39 ± 0.12 ng/mL, and 0.33 ± 0.07 ng/mL, respectively, vs. 0.88 ± 0.31 ng/mL; p < 0.05). Interleukin-1β levels in liver tissues were decreased among the probiotics and KRG groups. The tumor necrosis factor-α level of liver tissue was decreased in the KRG group.ConclusionThe pathological findings showed that alcohol-induced steatosis was significantly reduced by KRG and urushiol. As these agents improve immunologic capacity, they may be considered in potential anti-ALD treatments

Inhibitory Effect of Inflexinol on Nitric Oxide Generation and iNOS Expression via Inhibition of NF-κB Activation

Inflexinol, an ent-kaurane diterpenoid, was isolated from the leaves of Isodon excisus. Many diterpenoids isolated from the genus Isodon (Labiatae) have antitumor and antiinflammatory activities. We investigated the antiinflammatory effect of inflexinol in RAW 264.7 cells and astrocytes. As a result, we found that inflexinol (1, 5, 10 μM) suppressed the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) as well as the production of nitric oxide (NO) in LPS-stimulated RAW 264.7 cells and astrocytes. Consistent with the inhibitory effect on iNOS and COX-2 expression, inflexinol also inhibited transcriptional and DNA binding activity of NF-κB via inhibition of IκB degradation as well as p50 and p65 translocation into nucleus. These results suggest that inflexinol inhibits iNOS and COX-2 expression through inhibition of NF-κB activation, thereby inhibits generation of inflammatory mediators in RAW 264.7 cells and astrocytes, and may be useful for treatment of inflammatory diseases

Data Assimilation of AOD and Estimation of Surface Particulate Matters over the Arctic

In this study, more accurate information on the levels of aerosol optical depth (AOD) was calculated from the assimilation of the modeled AOD based on the optimal interpolation method. Additionally, more realistic levels of surface particulate matters over the Arctic were estimated using the assimilated AOD based on the linear relationship between the particulate matters and AODs. In comparison to the MODIS observation, the assimilated AOD was much improved compared with the modeled AOD (e.g., increase in correlation coefficients from −0.15–0.26 to 0.17–0.76 over the Arctic). The newly inferred monthly averages of PM10 and PM2.5 for April–September 2008 were 2.18–3.70 μg m−3 and 0.85–1.68 μg m−3 over the Arctic, respectively. These corresponded to an increase of 140–180%, compared with the modeled PMs. In comparison to in-situ observation, the inferred PMs showed better performances than those from the simulations, particularly at Hyytiala station. Therefore, combining the model simulation and data assimilation provided more accurate concentrations of AOD, PM10, and PM2.5 than those only calculated from the model simulations

A New Dual Circularly Polarized Feed Employing a Dielectric Cylinder-Loaded Circular Waveguide Open End Fed by Crossed Dipoles

This paper presents a new dual circularly polarized feed that provides good axial ratio over wide angles and low cross-polarized radiation in backward direction. A circular waveguide open end is fed with two orthogonally polarized waves in phase quadrature by a pair of printed crossed dipoles and a compact connectorized quadrature hybrid coupler. The waveguide aperture is loaded with a dielectric cylinder to reduce the cross-polarization beyond 90 degrees off the boresight. The fabricated feed has, at 5.5 GHz, 6.33-dBic copolarized gain, 3-dB beamwidth of 106°, 10-dB beamwidth of 195°, 3-dB axial ratio beamwidth of 215°, maximum cross-polarized gain of −21.4 dBic, and 27-dB port isolation. The reflection coefficient of the feed is less than −10 dB at 4.99–6.09 GHz

Data Assimilation of AOD and Estimation of Surface Particulate Matters over the Arctic

In this study, more accurate information on the levels of aerosol optical depth (AOD) was calculated from the assimilation of the modeled AOD based on the optimal interpolation method. Additionally, more realistic levels of surface particulate matters over the Arctic were estimated using the assimilated AOD based on the linear relationship between the particulate matters and AODs. In comparison to the MODIS observation, the assimilated AOD was much improved compared with the modeled AOD (e.g., increase in correlation coefficients from −0.15–0.26 to 0.17–0.76 over the Arctic). The newly inferred monthly averages of PM10 and PM2.5 for April–September 2008 were 2.18–3.70 μg m−3 and 0.85–1.68 μg m−3 over the Arctic, respectively. These corresponded to an increase of 140–180%, compared with the modeled PMs. In comparison to in-situ observation, the inferred PMs showed better performances than those from the simulations, particularly at Hyytiala station. Therefore, combining the model simulation and data assimilation provided more accurate concentrations of AOD, PM10, and PM2.5 than those only calculated from the model simulations