60 research outputs found
Student Recital (May 2, 2014)
Etude No. 62 / Mitchell Peters Nicole Desmarais, timpani
Malagueña from Spanish Dances, Op. 21 / Pablo de Sarasate Carla Mason, violin
Valse Venezolano No. 2 / Antonio Lauro Michael Cirino, guitar
Ballade / Albert Périlhou Nicole Mount, flute
Etude No. 8 / Heitor Villa-Lobos Dylan Mowry, guitar
Sicilienne and Rigaudon / Fritz Kreisler Tempo di Allegretto and Allegro Megan Polk, violin
Cavatine, Op. 144 / Camile Saint-Saëns Cody O’Toole, trombone
Concerto No. 1 in A minor / Jean Baptiste Accolaÿ Gail Colombo, violin
Das Wohltemperierte Klavier II, BWV 886 / Johann Sebastian Bach Prelude and Fugue in Ab Major Jiaying Zhu, piano
Cello Suite No. 1, BWV 1007 / J. S. Bach Prelude Killian Kerrigan, guitar
El Colibri / Julio S. Sagreras Brian Picher, guitar
Maria Wiegenlied / Max Reger Se Tu M’ami / Giovanni Battista Pergolesi Brooke Speigel, soprano
Nocturnal After John Dowland / Benjamin Britten Passacaglia and Theme Jim Davidson, guitar
Spring / Ivor Gurney Lied Der Mignon, D. 877, No. 3 / Franz Schubert Mackenzie Leahy, soprano
Ma Mère L’oye (Mother Goose Suite) / Maurice Ravel Pavane de la Belle au bois dormant (Lent) Petit Poucet. (Très modéré) Laideronnette, Impératrice des pagodes ( Mouvement de marche) Jiaying Zhu, piano Alexander Heinrich, piano
Waltz in Db Major, Op. 64, No. 1 / Frederic Chopin Brenner Campos, piano
Three Songs Without Words / Paul Ben-Haim Arioso Ballad Shepardic Melody Colby DeWitt, alto saxophone
For You There is No Song / Leslie Adams Deborah / Gene Bone Justine Smigel, sopranohttps://vc.bridgew.edu/student_concerts/1065/thumbnail.jp
The Colocalization Potential of HIV-Specific CD8+ and CD4+ T-Cells is Mediated by Integrin β7 but Not CCR6 and Regulated by Retinoic Acid
CD4+ T-cells from gut-associated lymphoid tissues (GALT) are major targets for HIV-1 infection. Recruitment of excess effector CD8+ T-cells in the proximity of target cells is critical for the control of viral replication. Here, we investigated the colocalization potential of HIV-specific CD8+ and CD4+ T-cells into the GALT and explored the role of retinoic acid (RA) in regulating this process in a cohort of HIV-infected subjects with slow disease progression. The expression of the gut-homing molecules integrin β7, CCR6, and CXCR3 was identified as a “signature” for HIV-specific but not CMV-specific CD4+ T-cells thus providing a new explanation for their enhanced permissiveness to infection in vivo. HIV-specific CD8+ T-cells also expressed high levels of integrin β7 and CXCR3; however CCR6 was detected at superior levels on HIV-specific CD4+
versus CD8+ T-cells. All trans RA (ATRA) upregulated the expression of integrin β7 but not CCR6 on HIV-specific T-cells. Together, these results suggest that HIV-specific CD8+ T-cells may colocalize in excess with CD4+ T-cells into the GALT via integrin β7 and CXCR3, but not via CCR6. Considering our previous findings that CCR6+CD4+ T-cells are major cellular targets for HIV-DNA integration in vivo, a limited ability of CD8+ T-cells to migrate in the vicinity of CCR6+CD4+ T-cells may facilitate HIV replication and dissemination at mucosal sites
Diving into the vertical dimension of elasmobranch movement ecology
Knowledge of the three-dimensional movement patterns of elasmobranchs is vital to understand their ecological roles and exposure to anthropogenic pressures. To date, comparative studies among species at global scales have mostly focused on horizontal movements. Our study addresses the knowledge gap of vertical movements by compiling the first global synthesis of vertical habitat use by elasmobranchs from data obtained by deployment of 989 biotelemetry tags on 38 elasmobranch species. Elasmobranchs displayed high intra- and interspecific variability in vertical movement patterns. Substantial vertical overlap was observed for many epipelagic elasmobranchs, indicating an increased likelihood to display spatial overlap, biologically interact, and share similar risk to anthropogenic threats that vary on a vertical gradient. We highlight the critical next steps toward incorporating vertical movement into global management and monitoring strategies for elasmobranchs, emphasizing the need to address geographic and taxonomic biases in deployments and to concurrently consider both horizontal and vertical movements
Genomic investigations of unexplained acute hepatitis in children
Since its first identification in Scotland, over 1,000 cases of unexplained paediatric hepatitis in children have been reported worldwide, including 278 cases in the UK1. Here we report an investigation of 38 cases, 66 age-matched immunocompetent controls and 21 immunocompromised comparator participants, using a combination of genomic, transcriptomic, proteomic and immunohistochemical methods. We detected high levels of adeno-associated virus 2 (AAV2) DNA in the liver, blood, plasma or stool from 27 of 28 cases. We found low levels of adenovirus (HAdV) and human herpesvirus 6B (HHV-6B) in 23 of 31 and 16 of 23, respectively, of the cases tested. By contrast, AAV2 was infrequently detected and at low titre in the blood or the liver from control children with HAdV, even when profoundly immunosuppressed. AAV2, HAdV and HHV-6 phylogeny excluded the emergence of novel strains in cases. Histological analyses of explanted livers showed enrichment for T cells and B lineage cells. Proteomic comparison of liver tissue from cases and healthy controls identified increased expression of HLA class 2, immunoglobulin variable regions and complement proteins. HAdV and AAV2 proteins were not detected in the livers. Instead, we identified AAV2 DNA complexes reflecting both HAdV-mediated and HHV-6B-mediated replication. We hypothesize that high levels of abnormal AAV2 replication products aided by HAdV and, in severe cases, HHV-6B may have triggered immune-mediated hepatic disease in genetically and immunologically predisposed children
The Transgressive Girl
This paper imagines the Internet as a potentially utopian girl-space by looking at how girls, and pop-cultural depictions of girls, use the language, signs, and symbols of the Internet, an inherently patriarchal system, in transgressive ways. I propose the 1990s media representations as the touchstone moment when the conditions of possibility for imagining the hacker as a weaponized girl emerged visually in popular culture. The girl, exemplified by various figures within popular television and film culture, is a precursor to and postulates an entry point into the ways Internet today is used in transgressive nature
Asymmetrical Flow Field-Flow Fractionation Methods for Quantitative Determination and Size Characterization of Thiols and for Mercury Size Speciation Analysis in Organic Matter-Rich Natural Waters
Asymmetrical flow field-flow fractionation (AF4) efficiently separates various macromolecules and nano-components of natural waters according to their hydrodynamic sizes. The online coupling of AF4 with fluorescence (Fluo) and UV absorbance (UV) detectors (FluoD and UVD, respectively) and inductively coupled plasma–mass spectrometry (ICP-MS) provides multidimensional information. This makes it a powerful tool to characterize and quantify the size distributions of organic and inorganic nano-sized components and their interaction with trace metals. In this study, we developed a method combining thiol labeling by monobromo(trimethylammonio)bimane bromide (qBBr) with AF4–FluoD to determine the size distribution and the quantities of thiols in the macromolecular dissolved organic matter (DOM) present in highly colored DOM-rich water sampled from Shuya River and Lake Onego, Russia. We found that the qBBr-labeled components of DOM (qB-DOM) were of humic type, characterized by a low hydrodynamic size ( d h &lt; 2 nm), and have concentrations &lt;0.3 μM. After enrichment with mercury, the complexes formed between the nano-sized components and Hg were analyzed using AF4–ICP-MS. The elution profile of Hg followed the distribution of the UV-absorbing components of DOM, characterized by slightly higher sizes than qB-DOM. Only a small proportion of Hg was associated with the larger-sized components containing Fe and Mn, probably inorganic oxides that were identified in most of the samples from river to lake. The size distribution of the Hg–DOM complexes was enlarged when the concentration of added Hg increased (from 10 to 100 nM). This was explained by the presence of small iron oxides, overlapping the size distribution of Hg–DOM, on which Hg bound to a small proportion. In addition, to provide information on the dispersion of macromolecular thiols in colored DOM-rich natural water, our study also illustrated the potential of AF4–FluoD–UVD–ICP-MS to trace or quantify dynamic changes while Hg binds to the natural nano-colloidal components of surface water. </p
Evaluation of Bioprinted Autologous Cartilage Grafts in an Immunocompetent Rabbit Model
The gold standard of auricular reconstruction involves manual graft assembly from autologous costal cartilage. The intervention may require multiple surgical procedures and lead to donor-site morbidity, while the outcome is highly dependent on individual surgical skills. A tissue engineering approach provides the means to produce cartilage grafts of a defined shape from autologous chondrocytes. The use of autologous cells minimizes the risk of host immune response; however, factors such as biomaterial compatibility and in vitro maturation of the tissue-engineered (TE) cartilage may influence the engraftment and shape-stability of TE implants. Here, this work tests the biocompatibility of bioprinted autologous cartilage constructs in a rabbit model. The TE cartilage is produced by embedding autologous auricular chondrocytes into hyaluronan transglutaminase (HATG) based bioink, previously shown to support chondrogenesis in human auricular chondrocytes in vitro and in immunocompromised xenotransplantation models in vivo. A drastic softening and loss of cartilage markers, such as sulfated glycosaminoglycans (GAGs) and collagen type II are observed. Furthermore, fibrous encapsulation and partial degradation of the transplanted constructs are indicative of a strong host immune response to the autologous TE cartilage. The current study thus illustrates the crucial importance of immunocompetent autologous animal models for the evaluation of TE cartilage function and compatibility.ISSN:2366-398
Characterizing lapine meniscal tissue: A regional comparison between normal medial and lateral menisci
The meniscus is comprised of two semilunar disks resting between the articular surface of the tibial plateaus and femoral condyles within the knee joint of each leg [1–3]. Both the medial and lateral menisci play a vital role in maintaining the joint’s integrity by distributing loads, stabilizing and lubricating the joint, and proprioceptive functions [2,3]. While the meniscus is found in many animals, morphological variations are present between species, indicating differences in the biomechanics of the joint [1,2]. These anatomical variations have not been quantified and, thus, remain unlinked to further structural changes that occur with injury. The goals of this study were aimed towards characterizing the normal lapine meniscal tissue using regional comparisons for tissue area and cell density measurements. The preliminary data from this research will be used as a comparison against future animal injury models. It was hypothesized that a difference would be observed between anterior, central, and posterior divisions in the normal lapine meniscus.</jats:p
Regional glycosaminoglycan coverage of healthy rabbit menisci
The meniscus is an important load-bearing structure in the knee, as it provides load distribution and cushioning properties during weight-bearing activities. The compressive modulus and permeability of the meniscus is attributed to the tissue’s glycosaminoglycan (GAG) content, as charged proteoglycan side chains allow for tissue swelling and resistance to compression [1]. The distribution of sulphated GAGs throughout the meniscus has not been thoroughly documented. Although load differs across the knee joint, few researchers have investigated medial/lateral and coronal differences in meniscal architecture and GAG distribution [2, 3]. It is hypothesized that the distribution of positive histological staining for sulfated GAGs will differ across spatial regions of rabbit menisci. Primarily, it is hypothesized that regions of the menisci that likely see higher loading will demonstrate an increase in sulfated GAG-positive staining area.</jats:p
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