523 research outputs found
High quality ultrafast transmission electron microscopy using resonant microwave cavities
Ultrashort, low-emittance electron pulses can be created at a high repetition
rate by using a TM deflection cavity to sweep a continuous beam across
an aperture. These pulses can be used for time-resolved electron microscopy
with atomic spatial and temporal resolution at relatively large average
currents. In order to demonstrate this, a cavity has been inserted in a
transmission electron microscope, and picosecond pulses have been created. No
significant increase of either emittance or energy spread has been measured for
these pulses.
At a peak current of pA, the root-mean-square transverse normalized
emittance of the electron pulses is m rad in the direction parallel to the streak of the cavity, and
m rad in the perpendicular
direction for pulses with a pulse length of 1.1-1.3 ps. Under the same
conditions, the emittance of the continuous beam is
m rad.
Furthermore, for both the pulsed and the continuous beam a full width at half
maximum energy spread of eV has been measured
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Farmers' management of rice varietal diversity in the mid-hills of Nepal: implications for on-farm conservation and crop improvement
Season-long monitoring of on-farm rice (Oryza sativa, L.) plots in Nepal explored farmers' decision-making process on the deployment of varieties to agroecosystems, application of production inputs to varieties, agronomic practices and relationship between economic return and area planted per variety. Farmers deploy varieties [landraces (LRs) and modern varieties (MVs)] to agroecosystems based on their understanding of characteristics of varieties and agroecosystems, and the interaction between them. In marginal growing conditions, LRs can compete with MVs. Within an agroecosystem, economic return and area planted to varieties have positive relationship, but this is not so between agroecosystems. LRs are very diverse on agronomic and economic traits; therefore, they cannot be rejected a priori as inferior materials without proper evaluation. LRs have to be evaluated for useful traits and utilized in breeding programmes to generate farmer-preferred materials for marginal environments and for their conservation on-farm
Theory and particle tracking simulations of a resonant radiofrequency deflection cavity in TM mode for ultrafast electron microscopy
We present a theoretical description of resonant radiofrequency (RF)
deflecting cavities in TM mode as dynamic optical elements for
ultrafast electron microscopy. We first derive the optical transfer matrix of
an ideal pillbox cavity and use a Courant-Snyder formalism to calculate the 6D
phase space propagation of a Gaussian electron distribution through the cavity.
We derive closed, analytic expressions for the increase in transverse emittance
and energy spread of the electron distribution. We demonstrate that for the
special case of a beam focused in the center of the cavity, the low emittance
and low energy spread of a high quality beam can be maintained, which allows
high-repetition rate, ultrafast electron microscopy with 100 fs temporal
resolution combined with the atomic resolution of a high-end TEM. This is
confirmed by charged particle tracking simulations using a realistic cavity
geometry, including fringe fields at the cavity entrance and exit apertures
Design and characterization of dielectric filled TM microwave cavities for ultrafast electron microscopy
Microwave cavities oscillating in the TM mode can be used as dynamic
electron-optical elements inside an electron microscope. By filling the cavity
with a dielectric material it becomes more compact and power efficient,
facilitating the implementation in an electron microscope. However, the
incorporation of the dielectric material makes the manufacturing process more
difficult. Presented here are the steps taken to characterize the dielectric
material, and to reproducibly fabricate dielectric filled cavities. Also
presented are two versions with improved capabilities. The first, called a
dual-mode cavity, is designed to support two modes simultaneously. The second
has been optimized for low power consumption. With this optimized cavity a
magnetic field strength of 2.84 0.07 mT was generated at an input power
of 14.2 0.2 W. Due to the low input powers and small dimensions, these
dielectric cavities are ideal as electron-optical elements for electron
microscopy setups
Dual mode microwave deflection cavities for ultrafast electron microscopy
This paper presents the experimental realization of an ultrafast electron
microscope operating at a repetition rate of 75 MHz based on a single compact
resonant microwave cavity operating in dual mode. This elliptical cavity
supports two orthogonal TM modes with different resonance frequencies
that are driven independently. The microwave signals used to drive the two
cavity modes are generated from higher harmonics of the same Ti:Sapphire laser
oscillator. Therefore the modes are accurately phase-locked, resulting in
periodic transverse deflection of electrons described by a Lissajous pattern.
By sending the periodically deflected beam through an aperture, ultrashort
electron pulses are created at a repetition rate of 75 MHz. Electron pulses
with fs pulse duration are created with only W
of microwave input power; with normalized rms emittances of
pm rad and pm rad for
a peak current of nA. This corresponds to an rms normalized
peak brightness of A/m sr V, equal
to previous measurements for the continuous beam. In addition, the FWHM energy
spread of eV is also unaffected by the dual mode
cavity. This allows for ultrafast pump-probe experiments at the same spatial
resolution of the original TEM in which a 75 MHz Ti:Sapphire oscillator can be
used for exciting the sample. Moreover, the dual mode cavity can be used as a
streak camera or time-of-flight EELS detector with a dynamic range
From Concept to Field Tests: Accelerated Development of Multi-AUV Missions Using a High-Fidelity Faster-than-Real-Time Simulator
We designed and validated a novel simulator for efficient development of
multi-robot marine missions. To accelerate development of cooperative
behaviors, the simulator models the robots' operating conditions with
moderately high fidelity and runs significantly faster than real time,
including acoustic communications, dynamic environmental data, and
high-resolution bathymetry in large worlds. The simulator's ability to exceed a
real-time factor (RTF) of 100 has been stress-tested with a robust continuous
integration suite and was used to develop a multi-robot field experiment
Determination of the coefficients of Langevin models for inhomogeneous turbulent flows by three-dimensional particle tracking velocimetry and direct numerical simulation
A promising and, in terms of computer power, low-cost way of describing flow properties such as turbulent diffusion is by Langevin models. The development of such models requires knowledge of Lagrangian statistics of turbulent flows. Our aim is to det. Lagrangian statistics of inhomogeneous flows, as most turbulent flows found in practical applications are inhomogeneous. The present paper describes how a Lagrangian measurement technique, three-dimensional particle tracking velocimetry, has been developed and applied to the most common example of inhomogeneous flows: turbulent pipe flow. A new direct numerical simulation (DNS) code has been developed and exptl. results have been compared with results of this DNS code. The results concern Eulerian and Lagrangian velocity statistics at two Reynolds nos. Based on these, coeffs. of the Langevin model have been detd. and phys. consequences for Langevin modeling and turbulent dispersion have been explained. [on SciFinder (R)
Prokaryotic responses to a warm temperature anomaly in northeast subarctic Pacific waters
Recent studies on marine heat waves describe water temperature anomalies causing changes in food web structure, bloom dynamics, biodiversity loss, and increased plant and animal mortality. However, little information is available on how water temperature anomalies impact prokaryotes (bacteria and archaea) inhabiting ocean waters. This is a nontrivial omission given their integral roles in driving major biogeochemical fluxes that influence ocean productivity and the climate system. Here we present a time-resolved study on the impact of a large-scale warm water surface anomaly in the northeast subarctic Pacific Ocean, colloquially known as the Blob, on prokaryotic community compositions. Multivariate statistical analyses identified significant depth- and season-dependent trends that were accentuated during the Blob. Moreover, network and indicator analyses identified shifts in specific prokaryotic assemblages from typically particle-associated before the Blob to taxa considered free-living and chemoautotrophic during the Blob, with potential implications for primary production and organic carbon conversion and export. Traving et al. use small subunit ribosomal RNA gene sequencing to examine spatial and temporal trends in bacterial and archaeal community structure during a large marine warm water surface anomaly, the Blob. Their findings suggest that community structure shifted during the Blob, with taxa considered free-living and chemoautotrophic prevailing under these unusual conditions
The TgsGP gene is essential for resistance to human serum in Trypanosoma brucei gambiense
Trypanosoma brucei gambiense causes 97% of all cases of African sleeping sickness, a fatal disease of sub-Saharan Africa. Most species of trypanosome, such as T. b. brucei, are unable to infect humans due to the trypanolytic serum protein apolipoprotein-L1 (APOL1) delivered via two trypanosome lytic factors (TLF-1 and TLF-2). Understanding how T. b. gambiense overcomes these factors and infects humans is of major importance in the fight against this disease. Previous work indicated that a failure to take up TLF-1 in T. b. gambiense contributes to resistance to TLF-1, although another mechanism is required to overcome TLF-2. Here, we have examined a T. b. gambiense specific gene, TgsGP, which had previously been suggested, but not shown, to be involved in serum resistance. We show that TgsGP is essential for resistance to lysis as deletion of TgsGP in T. b. gambiense renders the parasites sensitive to human serum and recombinant APOL1. Deletion of TgsGP in T. b. gambiense modified to uptake TLF-1 showed sensitivity to TLF-1, APOL1 and human serum. Reintroducing TgsGP into knockout parasite lines restored resistance. We conclude that TgsGP is essential for human serum resistance in T. b. gambiense
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