Possible use of Punica granatum (Pomegranate) in cancer therapy

The intake of fruits has proven to reduce the risk and incidence of cancer worldwide and plays a crucial role in cancer prevention. Pomegranate (Punica granatum), which belongs to the Punicaceae family, is one such plant that contains beneficial nutrients as well as many bioactive components and important phytochemicals that can be attributed to cancer-related therapeutic purposes. Pomegranate possesses antioxidant, anti-inflammatory, anti-proliferative, anti-angiogenic, anti-invasive, and anti-metastatic properties, and induces apoptosis. It also down-regulates various signaling pathways such as NF-?B, PI3K/AKT/mTOR, and Wnt, and down-regulates the expression of genes that are responsible in cancer development, such as anti-apoptotic genes, MMPs, VEGF, c-met, cyclins, Cdks, and pro-inflammatory cytokines. Therefore, inclusion of the fruit in one's diet would assist in a healthy life protected from cancer and also act as an effective chemotherapeutic with no toxic side effects

NGAL is downregulated in oral squamous cell carcinoma and leads to increased survival, proliferation, migration and chemoresistance

Oral cancer is a major public health burden worldwide. The lack of biomarkers for early diagnosis has increased the difficulty in managing this disease. Recent studies have reported that neutrophil gelatinase-associated lipocalin (NGAL), a secreted glycoprotein, is upregulated in various tumors. In our study, we found that NGAL was significantly downregulated in primary malignant and metastatic tissues of oral cancer in comparison to normal tissues. The downregulation of NGAL was strongly correlated with both degree of differentiation and stage (I–IV); it can also serve as a prognostic biomarker for oral cancer. Additionally, tobacco carcinogens were found to be involved in the downregulation of NGAL. Mechanistic studies revealed that knockdown of NGAL increased oral cancer cell proliferation, survival, and migration; it also induced resistance against cisplatin. Silencing of NGAL activated mammalian target of rapamycin (mTOR)signaling and reduced autophagy by the liver kinase B1 (LKB1)-activated protein kinase (AMPK)-p53-Redd1 signaling axis. Moreover, cyclin-D1, Bcl-2, and matrix metalloproteinase-9 (MMP-9) were upregulated, and caspase-9 was downregulated, suggesting that silencing of NGAL increases oral cancer cell proliferation, survival, and migration. Thus, from our study, it is evident that downregulation of NGAL activates the mTOR pathway and helps in the progression of oral cancer

Low energy radioactive ion beams at SPES for nuclear physics and medical applications

Over the past decades many accelerator facilities have been built in order to produce radioactive nuclei. Among the falcility under construction, SPES (Selective Production of Exotic Species) is the Italian ISOL (Isotope Separation On Line) facility in the installation phase in these years in the Laboratori Nazionali di Legnaro. The innovative aspect of this facility is that the radioactive beam produced by fission induced by the proton beam, produced by a high power cyclotron, interact with a multi-disks uranium carbide target. The formed RIB will be sent directly to the low energy experimental area and, afterwards, to the post-acceleration complex. Currently the installation program concerning the SPES RIB source provides the set-up of the apparatus around the production bunker. The main objective of SPES project is to provide, in the next years, the first low-energy radioactive beams for beta decay experiments using the b-DS (beta Decay Station) set-up and for radiopharmaceutical applications by means of the IRIS (ISOLPHARM Radioactive Implantation Station) apparatus. In this work, all the specific issues related to the SPES RIB and the Low Energy beam lines will be reported. The main RIB systems, such as ion source systems, target-handling devices and the installation of low energy transport line, will be presented in detail

Laser photo-ionization study of nat Ag using opto-galvanic signal at SPES offline laser lab

Resonant Ionization Laser Ion Source (RILIS) is one of themost advancing techniques for the production of radioactive ion beams (RIBs) in ISOL facilities. SPES project at INFN-LNL is a second generation ISOL facility which aims to produce several isotopes in a couple of years. Within the framework of this project, resonant photo-ionization schemes of several elements are studied in the offline laser lab, to be later implemented in the SPES Laser Ion Source. Silver is one of the elements being studied for the stated purpose. In this article, we report a resonant photo-ionization scheme of silver testedwith a hollowcathode lamp (HCL). Evidence of high lying Rydberg states around 60945.32 cm−1 has also been observed by studying the fast opto-galvanic signal detected

Aerosols behaviour in sensitive areas of the northwestern Himalaya—A case of Kullu-Manali tourist complex, India

332-340Total suspended particulate (TSP) matter on fortnightly basis throughout the year and mass size distribution of aerosols as well as ultrafine aerosols on weekly basis in the months of May and June were monitored during 1996-2003 at different altitudinal locations of the Kullu-Manali tourist complex in the northwestern Himalaya. Concentration of TSP ranged from 35.8 (August 1996) to 207.3 μg m-3 (June 2003) at Mohal [1150 m from average sea level (ASL)] and from 31.7 (July 2003) to 239 μg m-3 (April 2001) at Manali (2050 m ASL). The mass size distribution of aerosols showed a bimodal distribution having one peak in the coarse size range (3.3-9 μm dia) and the other in the fine size range (0.08-2.1μm dia] at both the locations. Ultrafine aerosol (UA) (0.001-0.1 μ m radius) concentrations were found to be highest at the lowest experimental altitude site (Mohal) and vice versa. The diurnal variation of UA concentration for three years showed that the concentration ranges from 2640 (at 0500 hrs LT) to 5160 Number (N) cm-3 (at 1300 hrs LT) at Mohal and from 400 (at 0400 hrs LT) to 2190N cm-3 (at 1300 hrs LT) at Kothi. On an average, TSP crossed its permissible limit set by National Ambient Air Quality Standard (NAAQS) level in the sensitive areas such as Kullu-Manali hill spots. Bimodal nature mass size distribution indicates two important sources contributing in total aerosols-the fine mode, primarily due to anthropogenic activities and the coarse mode aerosols, mainly due to natural sources. Large number of concentration of ultrafine particles indicates the presence of air pollutants more low altitudes as compared to high altitudes

Perception of Role of Oral and Maxillofacial Surgery Among Healthcare Professionals

Introduction: Oral and maxillofacial surgery (OMFS) is one of the oldest dental specialties. When compared to other surgical specialties, it is less popular among the healthcare professionals. Methods and materials: A questionnaire survey was conducted among 480 healthcare Professionals in Manipur, under four categories – dental students and practitioners, medical students and practitioners. Each group contains 120 participants and they were asked to select appropriate answers for 16 questions. 38 participants don't respond to the questionnaire which gives a total number of 442 participants. Data analysis is done using SPSS software. Results:Analysis of the data shows that 74.4% of participants were aware of various specialties of dentistry and 72.6% of them know about OMFS. For the nine clinical scenario based questions, almost all of them had opted OMFS for third molar removal, fractures of maxillofacial region and oral cancer. But most of them were unaware of the role of OMFS in cosmetic surgeries. Conclusion: Even though OMFS is a vast specialty, most of the healthcare professionals are unaware of its scope

New solid state laser system for SPES: Selective Production of Exotic Species project at Laboratori Nazionali di Legnaro

The Selective Production of Exotic Species project is under construction at Laboratori Nazionali di Legnaro-INFN. The aim of the collaboration is to produce highly pure Radioactive Ion Beams (RIBs) from fission fragments of a uranium carbide (UCx) target activated by a cyclotron proton beam. In order to select a specific atomic species, the main tool to be applied is the resonant laser ionization technique. We have just completed the installation of a dedicated all solid state laser system whose elements are tunable to transitions of all the elements/isotopes of interest for the project. The new laser system is based on three Titanium:sapphire laser sources, independently pumped by three Nd:YLF pump lasers, and it can be coupled to two high harmonic generation (second harmonic generation, third harmonic generation, and fourth harmonic generation) setups. The power, wavelength, and position of the laser beams are continuously monitored and stabilized by using automated active systems to improve the beam production stability of RIBs. This paper presents the main features of the laser system and examples of application of a laser ion source, including a first demonstration of photoionization of stable silver, one of the most requested elements for RIB application

A NEW PRODUCTION METHOD OF HIGH SPECIFIC ACTIVITY RADIONUCLIDES TOWARDS INNOVATIVE RADIOPHARMACEUTICALS: THE ISOLPHARM PROJECT

Radionuclides of interest in nuclear medicine are generally produced in cyclotrons or nuclear reactors, with associated issues such as highly enriched target costs and undesired contaminants. The ISOLPHARM project (ISOL technique for radioPHARMaceuticals) explores the feasibility of producing extremely high specific activity β-emitting radionuclides as radiopharmaceutical precursors. This technique is expected to produce radiopharmaceuticals very hardly obtained in standard production facilities. Radioactive isotopes will be obtained from nuclear reactions induced by accelerating 40 MeV protons in a cyclotron to collide on a UCx target. By means of: high working temperatures and high vacuum conditions, the migration of the radioactive elements towards an ion source, a potential difference up to 40 kV, and a mass separation device, an isobaric beam of desired radionuclides will be produced and implanted on a deposition target. The availability of innovative isotopes can potentially open a new generation of radiopharmaceuticals, based on nuclides never studied so far. Among these, a very promising isotope could be Ag-111, a β- emitter with a half-life (7.45 d), an average β- energy of 360 keV, a tissue penetration of around 1 mm, and a low percentage of γ-emission. The proof of principle studies on Ag-111 production and radiolabeling are currently under investigation in the ISOLPHARM_EIRA project, where both its production and possible application as a radiopharmaceutical precursor will be evaluated in its computational/physics, radiochemistry, and radiobiology tasks. Currently, innovative macromolecules meeting the specific requirements for the chelation and targeted delivery of Ag-111 are being developed, which will be further tested in vitro on 2D and 3D models, as well as in vivo for their pharmacokinetics and therapeutic potential onto xenograft models