New Method for Obtaining a Culture of Peritoneal Mast Cells

Актуальным является разработка методики получения культуры животных клеток, которую впоследствии можно будет использовать в качестве экспериментальной модели для проведения как фундаментальных исследований, так и доклинических испытаний лекарственных средств. Таковой является и разработка методики выделения линии перитонеальных тучных клеток из перитонеальной жидкости и ее культивирования в специализированных условиях. Полученная культура является подходящей экспериментальной моделью для проведения фундаментальных исследований тучных клеток – изучения их свойств и их взаимодействия с различными биологически активными веществами и другими клетками в со-культуре in vitro, и для проведения доклинических исследований специализированных лекарственных препаратов на ней.Currently, the development of a method for obtaining a culture of animal cells, which can later be used as an experimental model for conducting both basic research and preclinical trials of drugs, is urgent. The development of a technique for isolating a line of peritoneal mast cells from the peritoneal fluid and its cultivation in specialized conditions is also urgent. The resulting mast cell culture is a suitable experimental model for fundamental research - studying the properties of mast cells and their interaction with various biologically active substances and other cells in сo-culture in vitro, and for conducting preclinical studies of specialized drugs on it

Regulation of Tcf7l1 DNA Binding and Protein Stability as Principal Mechanisms of Wnt/β-Catenin Signaling

SummaryWnt/β-catenin signal transduction requires direct binding of β-catenin to Tcf/Lef proteins, an event that is classically associated with stimulating transcription by recruiting coactivators. This molecular cascade plays critical roles throughout embryonic development and normal postnatal life by affecting stem cell characteristics and tumor formation. Here, we show that this pathway utilizes a fundamentally different mechanism to regulate Tcf7l1 (formerly named Tcf3) activity. β-catenin inactivates Tcf7l1 without a switch to a coactivator complex by removing it from DNA, which leads to Tcf7l1 protein degradation. Mouse genetic experiments demonstrate that Tcf7l1 inactivation is the only required effect of the Tcf7l1-β-catenin interaction. Given the expression of Tcf7l1 in pluripotent embryonic and adult stem cells, as well as in poorly differentiated breast cancer, these findings provide mechanistic insights into the regulation of pluripotency and the role of Wnt/β-catenin in breast cancer

A MODIFIED MAST CELL CULTURE METHOD TO CREATE AN EXPERIMENTAL MODEL

Methods of obtaining a mast cell line from the mouse peritoneal fluid and cultivation it in specialized conditions for creating an experimental model are presented

Comparison of UNet, ENet, and BoxENet for Segmentation of Mast Cells in Scans of Histological Slices

Deep neural networks show high accuracy in the problem of semantic and instance segmentation of biomedical data. However, this approach is computationally expensive. The computational cost may be reduced with network simplification after training or choosing the proper architecture, which provides segmentation with less accuracy but does it much faster. In the present study, we analyzed the accuracy and performance of UNet and ENet architectures for the problem of semantic image segmentation. In addition, we investigated the ENet architecture by replacing of some convolution layers with box-convolution layers. The analysis performed on the original dataset consisted of histology slices with mast cells. These cells provide a region for segmentation with different types of borders, which vary from clearly visible to ragged. ENet was less accurate than UNet by only about 1-2%, but ENet performance was 8-15 times faster than UNet one. © 2019 IEEE

Recommendations for conducting practical training on the discipline "disaster medicine" in the format of a business game.

The article describes the general provisions and rules for conducting classes on the discipline "Disaster Medicine" in the form of a practical game.В статье рассмотрены общие положения и правила проведения занятия по дисциплине «Медицина катастроф» в виде практической игры

Assessment of physical and functional development in children of primary and senior school age engaged in sports school

The article presents the results of a study of physical and functional development in schoolchildren involved in the sports sections: taekwondo and skiing. The comparative characteristics of these groups of children.В статье приведены результаты исследования физического и функционального развития у школьников, занимающихся в спортивных секциях: тхэквондо и лыжи. Приведена сравнительная характеристика данных групп детей

Assessment of physical and functional development in children of primary and senior school age engaged in sports school

The article presents the results of a study of physical and functional development in schoolchildren involved in the sports sections: taekwondo and skiing. The comparative characteristics of these groups of children.В статье приведены результаты исследования физического и функционального развития у школьников, занимающихся в спортивных секциях: тхэквондо и лыжи. Приведена сравнительная характеристика данных групп детей

DEVELOPMENT OF A TECHNIQUE FOR ISOLATING PERITUBULAR MYOID CELLS FROM THE RAT TESTIS IN ORDER TO OBTAIN A PRIMARY CULTURE

A technique for obtaining a primary cell culture of the peritubular myoid cells has been developed and information has been obtained on the features of its cultivation under specialized conditions to create an experimental model

METHODOLOGICAL ASPECTS OF CONVENIENT MICROTOOLS MANUFACTURING FOR CELL CULTURES MANIPULATING

This paper presents a step-by-step plan for the manufacture of retention and injection microtools for conducting micropanipulations on various cell lines.Работа выполнена в рамках госзадания ИИФ УрО РАН (тема № АААА-А18-118020590108-7)

Spin chemistry investigation of peculiarities of photoinduced electron transfer in donor-acceptor linked system

Photoinduced intramolecular electron transfer in linked systems, (R,S)- and (S,S)-naproxen-N-methylpyrrolidine dyads, has been studied by means of spin chemistry methods [magnetic field effect and chemically induced dynamic nuclear polarization (CIDNP)]. The relative yield of the triplet state of the dyads in different magnetic field has been measured, and dependences of the high-field CIDNP of the N-methylpyrrolidine fragment on solvent polarity have been investigated. However, both (S,S)- and (R,S)-enantiomers demonstrate almost identical CIDNP effects for the entire range of polarity. It has been demonstrated that the main peculiarities of photoprocesses in this linked system are connected with the participation of singlet exciplex alongside with photoinduced intramolecular electron transfer in chromophore excited state quenching.This work was supported by the grants 08-03-00372 and 11-03-01104 of the Russian Foundation for Basic Research, and the grant of Priority Programs of the Russian Academy of Sciences, nr. 5.1.5.Magin, I.; Polyakov, N.; Khramtsova, E.; Kruppa, A.; Stepanov, A.; Purtov, P.; Leshina, T.... (2011). Spin chemistry investigation of peculiarities of photoinduced electron transfer in donor-acceptor linked system. Applied Magnetic Resonance. 41(2-4):205-220. https://doi.org/10.1007/s00723-011-0288-3S205220412-4J.S. Park, E. Karnas, K. Ohkubo, P. Chen, K.M. Kadish, S. Fukuzumi, C.W. Bielawski, T.W. Hudnall, V.M. Lynch, J.L. Sessler, Science 329, 1324–1327 (2010)S.Y. Reece, D.G. Nocera, Annu. Rev. Biochem. 78, 673–699 (2009)M.S. Afanasyeva, M.B. Taraban, P.A. Purtov, T.V. Leshina, C.B. Grissom, J. Am. Chem. Soc. 128, 8651–8658 (2006)M.A. Fox, M. Chanon, in Photoinduced Electron Transfer. C: Photoinduced Electron Transfer Reactions: Organic Substrates (Elsevier, New York, 1988), p. 754P.J. Hayball, R.L. Nation, F. Bochner, Chirality 4, 484–487 (1992)N. Suesa, M.F. Fernandez, M. Gutierrez, M.J. Rufat, E. Rotllan, L. Calvo, D. Mauleon, G. Carganico, Chirality 5, 589–595 (1993)A.M. Evans, J. Clin. Pharmacol. 36, 7–15 (1996)Y. Inoue, T. Wada, S. Asaoka, H. Sato, J.-P. Pete, Chem Commun. 4, 251–259 (2000)T. Yorozu, K. Hayashi, M. Irie, J. Am. Chem. Soc. 103, 5480–5548 (1981)N.J. Turro, in Modern Molecular Photochemistry (Benjamin/Cummings, San Francisco, 1978)K.M. Salikhov, Y.N. Molin, R.Z. Sagdeev, A.L. Buchachenko, in Spin Polarization and Magnetic Field Effects in Radical Reactions (Akademiai Kiado, Budapest, 1984), p. 419E.A. Weiss, M.A. Ratner, M.R. Wasielewski, J. Phys. Chem. A 107, 3639–3647 (2003)A.S. Lukas, P.J. Bushard, E.A. Weiss, M.R. Wasielewski, J. Am. Chem. Soc. 125, 3921–3930 (2003)R. Nakagaki, K. Mutai, M. Hiramatsu, H. Tukada, S. Nakakura, Can. J. Chem. 66, 1989–1996 (1988)M.C. Jim′enez, U. Pischel, M.A. Miranda, J. Photochem. Photobiol. C Photochem. Rev. 8, 128–142 (2007)S. Abad, U. Pischel, M.A. Miranda, Photochem. Photobiol. Sci. 4, 69–74 (2005)U. Pischel, S. Abad, L.R. Domingo, F. Bosca, M.A. Miranda, Angew. Chem. Int. Ed. 42, 2531–2534 (2003)G.L. Closs, R.J. Miller, J. Am. Chem. Soc. 101, 1639–1641 (1979)G.L. Closs, R.J. Miller, J. Am. Chem. Soc. 103, 3586–3588 (1981)M. Goez, Chem. Phys. Lett. 188, 451–456 (1992)I.F. Molokov, Y.P. Tsentalovich, A.V. Yurkovskaya, R.Z. Sagdeev, J. Photochem. Photobiol. A 110, 159–165 (1997)U. Pischel, S. Abad, M.A. Miranda, Chem. Commun. 9, 1088–1089 (2003)H. Hayashi, S. Nagakura, Bull. Chem. Soc. Jpn. 57, 322–328 (1984)Y. Sakaguchi, H. Hayashi, S. Nagakura, Bull. Chem. Soc. Jpn. 53, 39–42 (1980)H. Yonemura, H. Nakamura, T. Matsuo, Chem. Phys. Lett. 155, 157–161 (1989)N. Hata, M. Hokawa, Chem. Lett. 10, 507–510 (1981)M. Shiotani, L. Sjoeqvist, A. Lund, S. Lunell, L. Eriksson, M.B. Huang, J. Phys. Chem. 94, 8081–8090 (1990)E. Schaffner, H. Fischer, J. Phys. Chem. 100, 1657–1665 (1996)Y. Mori, Y. Sakaguchi, H. Hayashi, Chem. Phys. Lett. 286, 446–451 (1998)I.M. Magin, A.I. Kruppa, P.A. Purtov, Chem. Phys. 365, 80–84 (2009)K.K. Barnes, Electrochemical Reactions in Nonaqueous Systems (M. Dekker, New York, 1970), p. 560J. Bargon, J. Am. Chem. Soc. 99, 8350–8351 (1977)M. Goez, I. Frisch, J. Phys. Chem. A 106, 8079–8084 (2002)A.K. Chibisov, Russ. Chem. Rev. 50, 615–629 (1981)J. Goodman, K. Peters, J. Am. Chem. Soc. 107, 1441–1442 (1985)H. Cao, Y. Fujiwara, T. Haino, Y. Fukazawa, C.-H. Tung, Y. Tanimoto, Bull. Chem. Soc. Jpn. 69, 2801–2813 (1996)P.A. Purtov, A.B. Doktorov, Chem. Phys. 178, 47–65 (1993)A.I. Kruppa, O.I. Mikhailovskaya, T.V. Leshina, Chem. Phys. Lett. 147, 65–71 (1988)M.E. Michel-Beyerle, R. Haberkorn, W. Bube, E. Steffens, H. Schröder, H.J. Neusser, E.W. Schlag, H. Seidlitz, Chem. Phys. 17, 139–145 (1976)K. Schulten, H. Staerk, A. Weller, H.-J. Werner, B. Nickel, Z. Phys. Chem. 101, 371–390 (1976)K. Gnadig, K.B. Eisenthal, Chem. Phys. Lett. 46, 339–342 (1977)T. Nishimura, N. Nakashima, N. Mataga, Chem. Phys. Lett. 46, 334–338 (1977)M.G. Kuzmin, I.V. Soboleva, E.V. Dolotova, D.N. Dogadkin, High Eng. Chem. 39, 86–96 (2005